Preparation and characterization of collagen‐nanohydroxyapatite biocomposite scaffolds by cryogelation method for bone tissue engineering applications
Abstract
Recent efforts of bone repair focus on development of porous scaffolds for cell adhesion and proliferation. Collagen-nanohydroxyapatite (HA) scaffolds (70:30; 50:50; and 30:70 mass percentage) were produced by cryogelation technique using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide as crosslinking agents. A pure collagen scaffold was used as control. Morphology analysis revealed that all cryogels had highly porous structure with interconnective porosity and the nanoHA aggregates were randomly dispersed throughout the scaffold structure. Chemical analysis showed the presence of all major peaks related to collagen and HA in the biocomposites and indicated possible interaction between nanoHA aggregates and collagen molecules. Porosity analysis revealed an enhancement in the surface area as the nanoHA percentage increased in the collagen structure. The biocomposites showed improved mechanical properties as the nanoHA content increased in the scaffold. As expected, the swelling capacity decreased with the increase of nanoHA content. In vitro studies with osteoblasts cells showed that they were able to attach and spread in all cryogels surfaces. The presence of collagen-nanoHA biocomposites resulted in higher overall cellular proliferation compared to pure collagen scaffold. A statistically significant difference between collagen and collagen-nanoHA cryogels was observed after 21 day of cell culture. These innovative collagen-nanoHA cryogels could have potentially appealing application as scaffolds for bone regeneration.
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A nano-HA/collagen (30:70) scaffold showed increased osteoblast adhesion and mechanical property.
“Increased ostreoblast adhesion and mechanical property [112]”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [112]
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- Authors
- Rodrigues, S. C., Salgado, C. L., Sahu, A., Garcia, M. P., Fernandes, M. H., & Monteiro, F. J.
- Affiliations
- Universidade do Porto (PT)
- Corresponding author
- Rodrigues, Sandra C.
- ORCID
- Venue
- Journal of Biomedical Materials Research Part A
- Publisher
- Wiley
- Published
- 2012-09-24
- Volume
- 101A
- Issue
- 4
- Pages
- 1080-1094
- ISSN
- 1549-3296 (ISSN-L)
- 1552-4965
- OpenAlex
- W1985575261
- Cited by
- 127
- References
- 73
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 4 · 2023: 12 · 2022: 10 · 2021: 15 · 2020: 10 · 2019: 9 · 2018: 9 · 2017: 21 · 2016: 8 · 2015: 9 · 2014: 5 · 2013: 3
- FWCI
- 3.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biomedical Materials Research Part A (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- cryogelation, bone tissue engineering, bone regeneration, porous scaffolds, hydroxyapatite nanoparticles, collagen, osteoblast proliferation, mechanical properties, swelling power, surface area, osteoblast adhesion
- MeSH terms
- Animals, Bone and Bones, Bone Regeneration, Cattle, Cell Adhesion, Cell Line, Collagen, Materials Testing, Osteoblasts, Porosity, Durapatite, Tissue Engineering, Cell Proliferation, Mice, Nanocomposites, Tissue Scaffolds, Cryogels
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