Nanobiocomposite based on natural polyelectrolytes for bone regeneration
Open accessAbstract
We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration. The 3D porous-structure of the biocomposite scaffolds were confirmed by Scanning Electron Microscopy and Microtomography analysis. Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold. The hydrogel properties of the matrices were studied by swelling and mechanical tests, indicating that the scaffold presented a good mechanical behavior. The degradation test demonstrated that the material is slowly degraded, while the addition of nHA slightly influences the degradation of the scaffolds. Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content. On the other hand, no cytotoxic effect was observed when RAW264.7 cells were seeded on the scaffolds. Altogether, our results allow us to conclude that these nanobiocomposites are promising candidates to induce bone tissue regeneration.
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Natural biopolymers have organized structures and ligands that guide cells to bind and grow.
“Also, natural biopolymers have organized structures and ligands which guide the cells to bind and grow [17].”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [17]
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Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration.
Kavitha Sri, A., Arthi, C., Neya, N. R., & Hikku, G. S. (2023) · Biomedical Materials · doi:10.1088/1748-605x/acc99e · Cited by 57 · see citing works
- Authors
- Belluzo, M. S., Medina, L. F., Molinuevo, M. S., Cortizo, M. S., & Cortizo, A. M.
- Affiliations
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Universidad Nacional de La Plata (AR)
- Corresponding author
- Belluzo, M. Soledad
- Venue
- Journal of Biomedical Materials Research Part A
- Publisher
- Wiley
- Published
- 2020-03-29
- Volume
- 108
- Issue
- 7
- Pages
- 1467-1478
- ISSN
- 1549-3296 (ISSN-L)
- 1552-4965
- OpenAlex
- W3011890117
- Cited by
- 8
- References
- 40
- Citations by year
- 2023: 3 · 2022: 2 · 2021: 3
- FWCI
- 0.63
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Journal of Biomedical Materials Research Part A (publishedVersion)
- PubMed (publishedVersion)
- Conicet (submittedVersion, cc-by-nc-sa)
- Funders
- Fondo para la Investigación Científica y Tecnológica (PICT 2016‐1560)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- bionanocomposites, chitosan, carboxymethyl cellulose, hydroxyapatite nanoparticles, bone regeneration, hydrogel scaffolds, sonication, porous scaffolds, scaffold degradation, biocompatibility, mesenchymal progenitor cells, alkaline phosphatase activity, cytotoxicity
- MeSH terms
- RAW 264.7 Cells, Polyelectrolytes, Animals, Biocompatible Materials, Bone Regeneration, Carboxymethylcellulose Sodium, Cell Line, Durapatite, Chitosan, Mice, Tissue Scaffolds, Mesenchymal Stem Cells
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