Content-Dependent Osteogenic Response of Nanohydroxyapatite: An in Vitro and in Vivo Assessment within Collagen-Based Scaffolds

Cunniffe, Gráinne M.; Curtin, Caroline M.; Thompson, Emmet M.; Dickson, Glenn R.; O’Brien, Fergal J.

doi:10.1021/acsami.6b06596

Cited by 95Bone Tissue Engineering MaterialsCollagen: Extraction and CharacterizationTissue Engineering and Regenerative Medicine

Content-Dependent Osteogenic Response of Nanohydroxyapatite: An in Vitro and in Vivo Assessment within Collagen-Based Scaffolds

Cunniffe, Gráinne M., Curtin, Caroline M., Thompson, Emmet M., Dickson, Glenn R., O’Brien, Fergal J.·2016·ACS Applied Materials & Interfaces

DOI 10.1021/acsami.6b06596

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Abstract

The use of collagen-based scaffolds in orthopedic applications has been limited due to poor mechanical properties, but this may be overcome by the introduction of a stiffer supporting phase. Thus, we developed a synthesis technique to produce nonaggregating, stable nanohydroxyapatite (nHA) particles, permitting the fabrication of biomimetic-inspired scaffolds through the combination of nanosized HA with collagen, as found in native bone. This study evaluates the mechanical and biological impact of incorporating increasing concentrations of these nanoparticles into porous collagen scaffolds (1:1 and 5:1 weight ratios of nHA/collagen). Mechanical assessment demonstrated that increasing nHA incorporation correlated with increasing Young's moduli, which could be further amplified using cross-linking treatments. Typically, the porosity of a scaffold is sacrificed to produce a stiffer material; however, through the use of nanosized particles the inclusion of up to 5:1 nHA/collagen content still preserved the high 99% porosity of the composite scaffold, allowing for maximum cell infiltration. Moreover, increasing nHA presence induced significant bioactive responses, achieving superior cellular attachment and enhanced osteogenesis, promoting earlier expression of bone markers and cell-mediated mineralization versus nHA-free collagen controls. Interestingly, these content-dependent results observed in vitro did not directly translate in vivo. Instead, similar levels of bone formation were achieved within critical-sized rat calvarial defects, independent of nHA content, following acellular implantation. The addition of nHA, both 1:1 and 5:1, induced significantly higher levels of mineralization and de novo bone ingrowth versus collagen controls as demonstrated by microcomputed tomography, histological, and histomorphometric analyses. Ultimately, these results demonstrate the immense osteoinductivity of nonaggregated nanoparticles of HA incorporated into collagen-composite scaffolds and emphasize the importance of in vivo-based evaluation of therapies intended for clinical use.

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Structural and signaling molecules such as α 5 β 1 integrin and focal adhesion kinase that are important for bone formation easily interact with the -OH group of HA, leading to deposition of minerals.
“The structural, as well as signaling molecules such as α 5 β 1 integrin and focal adhesion kinase that are important for bone formation, easily interact with the -OH group of HA which in turn leads to the deposition of minerals [57].”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [57]

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Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915 · see citing works
Tissue engineering
Langer, R., & Vacanti, J. P. (1993) · Science · doi:10.1126/science.8493529 · Cited by 9,893 · see citing works
Scaffolds in tissue engineering bone and cartilage
Hutmacher, D. W. (2000) · Biomaterials · doi:10.1016/s0142-9612(00)00121-6 · Cited by 5,002 · see citing works
The effect of mean pore size on cell attachment, proliferation and migration in collagen–glycosaminoglycan scaffolds for bone tissue engineering
Murphy, C. M., Haugh, M. G., & O’Brien, F. J. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.09.063 · Cited by 2,057 · see citing works
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O’Brien, F. J., Harley, B. A. C., Yannas, I. V., & Gibson, L. J. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.02.052 · Cited by 1,336 · see citing works
Self-citation
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Webster, T. J. (2000) · Biomaterials · doi:10.1016/s0142-9612(00)00075-2 · Cited by 1,324 · see citing works
Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering
Wei, G., & Ma, P. X. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2003.12.005 · Cited by 1,271 · see citing works
Nanotechnology and nanomaterials: Promises for improved tissue regeneration
Zhang, L. G., & Webster, T. J. (2008) · Nano Today · doi:10.1016/j.nantod.2008.10.014 · Cited by 1,147 · see citing works
Natural‐Based Nanocomposites for Bone Tissue Engineering and Regenerative Medicine: A Review
Pina, S., Oliveira, J. M., & Reis, R. L. (2015) · Advanced Materials · doi:10.1002/adma.201403354 · Cited by 945 · see citing works
A biomimetic three-dimensional woven composite scaffold for functional tissue engineering of cartilage
Moutos, F. T., Freed, L. E., & Guilak, F. (2007) · Nature Materials · doi:10.1038/nmat1822 · Cited by 738 · see citing works
Surface chemistry modulates focal adhesion composition and signaling through changes in integrin binding
Keselowsky, B. G., Collard, D. M., & Garcı́a, A. J. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.01.062 · Cited by 627 · see citing works
Stimulation of osteoblast responses to biomimetic nanocomposites of gelatin-hydroxyapatite for tissue engineering scaffolds.
Kim, H., Kim, H.-E., & Salih, V. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.01.047 · Cited by 451 · see citing works
The effect of pore size on permeability and cell attachment in collagen scaffolds for tissue engineering
O’Brien, F. J., Harley, B. A. C., Waller, M. A., Yannas, I. V., Gibson, L. J., & Prendergast, P. J. (2006) · Technology and Health Care · doi:10.3233/thc-2007-15102 · Cited by 368 · see citing works
Self-citation
A study on the bioactivity of chitosan/nano-hydroxyapatite composite scaffolds for bone tissue engineering
Kong, L., Gao, Y., Lu, G., et al. (2006) · European Polymer Journal · doi:10.1016/j.eurpolymj.2006.08.009 · Cited by 331 · see citing works
Crosslinking and Mechanical Properties Significantly Influence Cell Attachment, Proliferation, and Migration Within Collagen Glycosaminoglycan Scaffolds
Haugh, M. G., Murphy, C. M., McKiernan, R. C., Altenbuchner, C., & O’Brien, F. J. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2010.0590 · Cited by 330 · see citing works
Self-citation
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Habibović, P., Sees, T. M., van den Doel, M., et al. (2006) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30712 · Cited by 315 · see citing works
The collagen receptor integrins have distinct ligand recognition and signaling functions
Heino, J. (2000) · Matrix Biology · doi:10.1016/s0945-053x(00)00076-7 · Cited by 299 · see citing works
The effect of dehydrothermal treatment on the mechanical and structural properties of collagen‐GAG scaffolds
Haugh, M. G., Jaasma, M. J., & O’Brien, F. J. (2008) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.31955 · Cited by 282 · see citing works
Self-citation
Addition of hydroxyapatite improves stiffness, interconnectivity and osteogenic potential of a highly porous collagen-based scaffold for bone tissue regeneration
Gleeson, J. P., Plunkett, N., & O’Brien, F. J. (2010) · European Cells and Materials · doi:10.22203/ecm.v020a18 · Cited by 255 · see citing works
Self-citation
Biodegradable HA-PLA 3-D porous scaffolds: Effect of nano-sized filler content on scaffold properties
Kothapalli, C. R., Shaw, M. T., & Wei, M. (2005) · Acta Biomaterialia · doi:10.1016/j.actbio.2005.06.005 · Cited by 247 · see citing works
Study of hydroxyapatite osteoinductivity with an osteogenic differentiation of mesenchymal stem cells
Lin, L., Chow, K. L., & Leng, Y. (2008) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.31994 · Cited by 237 · see citing works
A Collagen-glycosaminoglycan Scaffold Supports Adult Rat Mesenchymal Stem Cell Differentiation Along Osteogenic and Chondrogenic Routes
Farrell, E., O’Brien, F. J., Doyle, P. N., Fischer, J., Yannas, I. V., Harley, B. A. C., O'Connell, B. C., Prendergast, P. J., & Campbell, V. A. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.459 · Cited by 227 · see citing works
Self-citation
The healing of bony defects by cell-free collagen-based scaffolds compared to stem cell-seeded tissue engineered constructs
Lyons, F. G., Al-Munajjed, A. A., Kieran, S. M., Toner, M. E., Murphy, C. M., Duffy, G. P., & O’Brien, F. J. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.08.056 · Cited by 222 · see citing works
Self-citation
Osteoinduction of Human Mesenchymal Stem Cells by Bioactive Composite Scaffolds without Supplemental Osteogenic Growth Factors
Polini, A., Pisignano, D., Parodi, M., Quarto, R., & Scaglione, S. (2011) · PLoS ONE · doi:10.1371/journal.pone.0026211 · Cited by 211 · see citing works
Innovative Collagen Nano‐Hydroxyapatite Scaffolds Offer a Highly Efficient Non‐Viral Gene Delivery Platform for Stem Cell‐Mediated Bone Formation
Curtin, C., Cunniffe, G., Lyons, F., Bessho, K., Dickson, G., Duffy, G., & O'Brien, F. (2012) · Advances in Materials · doi:10.1002/adma.201103828 · Cited by 204 · see citing works
Self-citation
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Chen, G., Dong, C., Yang, L., & Lv, Y. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b02662 · Cited by 204 · see citing works
Hydroxyapatite reinforced collagen scaffolds with improved architecture and mechanical properties
Kane, R. J., Weiss‐Bilka, H. E., Meagher, M. J., Liu, Y., Gargac, J. A., Niebur, G. L., Wagner, D. R., & Roeder, R. K. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.01.031 · Cited by 202 · see citing works
Development and characterisation of a collagen nano-hydroxyapatite composite scaffold for bone tissue engineering
Cunniffe, G. M., Dickson, G. R., Partap, S., Stanton, K. T., & O’Brien, F. J. (2010) · Journal of Materials Science: Materials in Medicine · doi:10.1007/s10856-009-3964-1 · Cited by 199 · see citing works
Self-citation
The Sequential Expression Profiles of Growth Factors from Osteroprogenitors to Osteoblasts In Vitro
Huang, Z., Nelson, E. R., Smith, R. L., et al. (2007) · Tissue Engineering · doi:10.1089/ten.2006.0423 · Cited by 197 · see citing works
Cell–scaffold mechanical interplay within engineered tissue
Rosenfeld, D., & Levenberg, S. (2009) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2009.02.001 · Cited by 170 · see citing works
Polydopamine-Templated Hydroxyapatite Reinforced Polycaprolactone Composite Nanofibers with Enhanced Cytocompatibility and Osteogenesis for Bone Tissue Engineering
Gao, X., Song, J., Ji, P., Zhang, X., Li, X., Xu, X., Wang, M., Zhang, S., Deng, Y., Deng, F., et al. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b12413 · Cited by 170 · see citing works
Combinatorial Gene Therapy Accelerates Bone Regeneration: Non‐Viral Dual Delivery of VEGF and BMP2 in a Collagen‐Nanohydroxyapatite Scaffold
Curtin, C., Tierney, E. G., McSorley, K., Cryan, S., Duffy, G., & O'Brien, F. (2015) · Advanced Healthcare Materials · doi:10.1002/adhm.201400397 · Cited by 165 · see citing works
Self-citation
Differences between in vitro viability and differentiation and in vivo bone-forming efficacy of human mesenchymal stem cells cultured on PCL–TCP scaffolds
Rai, B., Lin, J., Lim, Z. X. H., Guldberg, R. E., Hutmacher, D. W., & Cool, S. M. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.07.001 · Cited by 157 · see citing works
Effect of surface roughness on osteogenesis in vitro and osseointegration in vivo of carbon fiber-reinforced polyetheretherketone–nanohydroxyapatite composite
Wei, S., Deng, Y., Liu, X., Xu, A., Wang, L., Luo, Z., Zheng, Y., Deng, F., Tang, Z., & Wei, J. (2015) · International Journal of Nanomedicine · doi:10.2147/ijn.s75557 · Cited by 151 · see citing works
Osteoinduction of hydroxyapatite/β-tricalcium phosphate bioceramics in mice with a fractured fibula
Cheng, L., Ye, F., Yang, R., Lu, X., Shi, Y., Li, L., Fan, H., & Bu, H. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2009.10.050 · Cited by 151 · see citing works
Blood vessel formation in the tissue-engineered bone with the constitutively active form of HIF-1α mediated BMSCs
Zou, D., Zhang, Z., He, J., Zhang, K., Ye, D., Han, W., Zhou, J., Wang, Y., Li, Q., Liu, X., et al. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.11.053 · Cited by 146 · see citing works
Nanotechnology for bone materials
Tran, N. L., & Webster, T. J. (2009) · Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.23 · Cited by 141 · see citing works
Enhanced Biocompatibility of PLGA Nanofibers with Gelatin/Nano-Hydroxyapatite Bone Biomimetics Incorporation
Li, D., Sun, H., Jiang, L., Zhang, K., Liu, W., Zhu, Y., Fangteng, J., Shi, C., Zhao, L., Sun, H., et al. (2014) · ACS Applied Materials & Interfaces · doi:10.1021/am5017792 · Cited by 135 · see citing works
Osteoblasts respond to hydroxyapatite surfaces with immediate changes in gene expression
Xie, J., Baumann, M. J., & McCabe, L. R. (2004) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30140 · Cited by 134 · see citing works
Fabrication and characterization of novel nano‐ and micro‐HA/PCL composite scaffolds using a modified rapid prototyping process
Heo, S., Kim, S., Wei, J., Hyun, Y., Yun, H., Kim, D., Shin, J. W., & Shin, J. (2008) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.31726 · Cited by 131 · see citing works
Adhesion of osteoblast-like cells on nanostructured hydroxyapatite
Okada, S., Ito, H., Nagai, A., Komotori, J., & Imai, H. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2009.07.037 · Cited by 130 · see citing works
Preparation and characterization of collagen‐nanohydroxyapatite biocomposite scaffolds by cryogelation method for bone tissue engineering applications
Rodrigues, S. C., Salgado, C. L., Sahu, A., Garcia, M. P., Fernandes, M. H., & Monteiro, F. J. (2013) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.34394 · Cited by 127 · see citing works
Probing the Osteoinductive Effect of Calcium Phosphate by Using an In Vitro Biomimetic Model
Chai, Y. C., Roberts, S. J., Schrooten, J., et al. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2010.0160 · Cited by 127 · see citing works
The synthesis and characterization of nanophase hydroxyapatite using a novel dispersant‐aided precipitation method
Cunniffe, G. M., O’Brien, F. J., Partap, S., Levingstone, T. J., Stanton, K. T., & Dickson, G. R. (2010) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.32931 · Cited by 106 · see citing works
Self-citation
Nanoceramic surface roughness enhances osteoblast and osteoclast functions for improved orthopaedic/dental implant efficacy
Webster, T. J., Siegel, R. W., & Bizios, R. (2001) · Scripta Materialia · doi:10.1016/s1359-6462(01)00873-9 · Cited by 105 · see citing works
Micro-/Nano- sized hydroxyapatite directs differentiation of rat bone marrow derived mesenchymal stem cells towards an osteoblast lineage
Huang, Y., Zhou, G., Zheng, L., et al. (2012) · Nanoscale · doi:10.1039/c2nr12072k · Cited by 101 · see citing works
Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration
Raucci, M. G., D’Antò, V., Guarino, V., Sardella, E., Zeppetelli, S., Favia, P., & Ambrosio, L. (2010) · Acta Biomaterialia · doi:10.1016/j.actbio.2010.04.018 · Cited by 87 · see citing works
Osteogenesis on nanoparticulate mineralized collagen scaffolds via autogenous activation of the canonical BMP receptor signaling pathway
Ren, X., Bischoff, D. S., Weisgerber, D. W., Lewis, M. S., Tu, V., Yamaguchi, D. T., Miller, T. A., Harley, B. A. C., & Lee, J. C. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.01.059 · Cited by 84 · see citing works
Characterization of nano hydroxyapatite/collagen surfaces and cellular behaviors
Zhu, X., Eibl, O., Scheideler, L., et al. (2006) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30706 · Cited by 81 · see citing works
Beads of collagen–nanohydroxyapatite composites prepared by a biomimetic process and the effects of their surface texture on cellular behavior in MG63 osteoblast-like cells
Tsai, S., Hsu, F., & Chen, P. (2008) · Acta Biomaterialia · doi:10.1016/j.actbio.2008.03.015 · Cited by 70 · see citing works
Effect of Roughness on in Situ Biomineralized CaP-Collagen Coating on the Osteogenesis of Mesenchymal Stem Cells
Zan, X., Sitasuwan, P., Feng, S., et al. (2016) · Langmuir · doi:10.1021/acs.langmuir.5b04245 · Cited by 68 · see citing works
Culturing Primary Human Osteoblasts on Electrospun Poly(lactic-co-glycolic acid) and Poly(lactic-co-glycolic acid)/Nanohydroxyapatite Scaffolds for Bone Tissue Engineering
Li, M., Liu, W., Sun, J., Xianyu, Y., Wang, J., Zhang, W., Zheng, W., Huang, D., Di, S., Long, Y., et al. (2013) · ACS Applied Materials & Interfaces · doi:10.1021/am401937m · Cited by 62 · see citing works
In Vitro Study of Surface Modified Poly(ethylene glycol)-Impregnated Sintered Bovine Bone Scaffolds on Human Fibroblast Cells
Pramanik, S., Ataollahi, F., Pingguan‐Murphy, B., et al. (2015) · Scientific Reports · doi:10.1038/srep09806 · Cited by 61 · see citing works
Contractile behavior of smooth muscle actin-containing osteoblasts in collagen-GAG matrices in vitro: implant-related cell contraction
Menard, C., Mitchell, S., & Spector, M. (2000) · Biomaterials · doi:10.1016/s0142-9612(00)00062-4 · Cited by 46 · see citing works
Biomineralization of Natural Collagenous Nanofibrous Membranes and Their Potential Use in Bone Tissue Engineering
Yang, M., Zhou, G., Castano‐Izquierdo, H., et al. (2014) · Journal of Biomedical Nanotechnology · doi:10.1166/jbn.2015.2038 · Cited by 36 · see citing works
Collagen–hydroxyapatite composite scaffolds for tissue engineering
Liu, C. (2015) · Elsevier eBooks · doi:10.1016/b978-1-78242-033-0.00010-9 · Cited by 34 · see citing works
The Osteogenesis of Bone Marrow Stem Cells on mPEG-PCL-mPEG/Hydroxyapatite Composite Scaffold via Solid Freeform Fabrication
Liao, H.-T., Chen, Y.-Y., Lai, Y.-T., Hsieh, M., & Jiang, C. (2014) · BioMed Research International · doi:10.1155/2014/321549 · Cited by 34 · see citing works
Mechanical Properties of Glass-Ceramic A-W - Polyethylene Composites: Effect of Filler Content
Juhasz, J. A., Best, S. M., Kawashita, M., et al. (2003) · Key engineering materials · doi:10.4028/www.scientific.net/kem.240-242.947 · Cited by 4 · see citing works
Study of Hydroxyapatite Osteoinduction with Osteo-Specific Gene Expression of Stem Cells
Lin, L. W., Chow, K. L., & Leng, Y. (2007) · Key engineering materials · doi:10.4028/www.scientific.net/kem.361-363.1005 · Cited by 2 · see citing works

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Chen, Y., Zhu, R., Ke, Q., et al. (2017) · Nanoscale · doi:10.1039/c7nr00601b · Cited by 68 · see citing works
Advancing bone tissue engineering one layer at a time: a layer-by-layer assembly approach to 3D bone scaffold materials
Sahebalzamani, M., Ziminska, M., McCarthy, H. O., Levingstone, T. J., Dunne, N., & Hamilton, A. (2022) · Biomaterials Science · doi:10.1039/d1bm01756j · Cited by 66 · see citing works
Recent development in multizonal scaffolds for osteochondral regeneration
Yu, L., Cavelier, S., Hannon, B., & Wei, M. (2023) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.01.012 · Cited by 66 · see citing works
Biodegradable PCL/fibroin/hydroxyapatite porous scaffolds prepared by supercritical foaming for bone regeneration
Díaz-Gómez, L. A., García‐González, C. A., Wang, J., Yang, F., Aznar‐Cervantes, S. D., Cenís, J. L., Reyes, R., Delgado, A., Évora, C., Concheiro, Á., et al. (2017) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2017.05.038 · Cited by 60 · see citing works
Natural protein bioinspired materials for regeneration of hard tissues
Xu, X., Chen, X., & Li, J. (2020) · Journal of Materials Chemistry B · doi:10.1039/d0tb00139b · Cited by 60 · see citing works
Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration.
Kavitha Sri, A., Arthi, C., Neya, N. R., & Hikku, G. S. (2023) · Biomedical Materials · doi:10.1088/1748-605x/acc99e · Cited by 57 · see citing works
Rational design of gelatin/nanohydroxyapatite cryogel scaffolds for bone regeneration by introducing chemical and physical cues to enhance osteogenesis of bone marrow mesenchymal stem cells
Shalumon, K. T., Liao, H.-T., Kuo, C., Wong, C.-B., Li, C.-J., Mini, P. A., & Chen, J. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.109855 · Cited by 57 · see citing works
Influences of the 3D microenvironment on cancer cell behaviour and treatment responsiveness: A recent update on lung, breast and prostate cancer models
Costard, L. S., Hosn, R. R., Ramanayake, H., O’Brien, F. J., & Curtin, C. M. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.01.023 · Cited by 57 · see citing works
Self-citation
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Sadowska, J. M., Power, R. N., Genoud, K. J., Matheson, A., González‐Vázquez, A., Costard, L. S., Eichholz, K. F., Pitacco, P., Hallegouet, T., Chen, G., et al. (2023) · Advanced Materials · doi:10.1002/adma.202307639 · Cited by 55 · see citing works
Self-citation
Bioengineered Hybrid Collagen Scaffold Tethered with Silver‐Catechin Nanocomposite Modulates Angiogenesis and TGF‐ β Toward Scarless Healing in Chronic Deep Second Degree Infected Burns
Kalirajan, C., & Thanikaivelan, P. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.202000247 · Cited by 53 · see citing works
Preclinical models for neuroblastoma: Advances and challenges
Nolan, J. C., Frawley, T., Tighe, J., Soh, H., Curtin, C. M., & Piskareva, O. (2020) · Cancer Letters · doi:10.1016/j.canlet.2020.01.015 · Cited by 52 · see citing works
Self-citation
<p>Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects</p>
Zhao, H., Tang, J., Zhou, D., Weng, Y., Qin, W., Liu, C., Lv, S., Wang, W., & Zhao, X. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s238800 · Cited by 51 · see citing works
Bioinspired Collagen Scaffolds in Cranial Bone Regeneration: From Bedside to Bench
Lee, J. C., & Volpicelli, E. J. (2017) · Advanced Healthcare Materials · doi:10.1002/adhm.201700232 · Cited by 51 · see citing works
Harnessing an Inhibitory Role of miR-16 in Osteogenesis by Human Mesenchymal Stem Cells for Advanced Scaffold-Based Bone Tissue Engineering
Castaño, I. M., Curtin, C. M., Duffy, G. P., & O’Brien, F. J. (2018) · Tissue Engineering Part A · doi:10.1089/ten.tea.2017.0460 · Cited by 48 · see citing works
Self-citation
3D-printed photoluminescent bioactive scaffolds with biomimetic elastomeric surface for enhanced bone tissue engineering
Chen, M., Zhao, F., Li, Y., et al. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.110153 · Cited by 43 · see citing works
Photo-crosslinkable, bone marrow-derived mesenchymal stem cells-encapsulating hydrogel based on collagen for osteogenic differentiation
Zhang, T., Chen, H., Zhang, Y., Zan, Y., Ni, T., Liu, M., & Pei, R. (2018) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2018.11.050 · Cited by 42 · see citing works
Rapid bone repair with the recruitment of CD206+M2-like macrophages using non-viral scaffold-mediated miR-133a inhibition of host cells
Castaño, I. M., Raftery, R. M., Chen, G., Cavanagh, B., Quinn, B., Duffy, G. P., O’Brien, F. J., & Curtin, C. M. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2020.03.042 · Cited by 42 · see citing works
Self-citation
Enhanced bioactivity and osteoinductivity of carboxymethyl chitosan/nanohydroxyapatite/graphene oxide nanocomposites
Zhang, Y., Xiao, C., Huang, Y., et al. (2018) · RSC Advances · doi:10.1039/c8ra00383a · Cited by 41 · see citing works
Nano-Silicate-Reinforced and SDF-1α-Loaded Gelatin-Methacryloyl Hydrogel for Bone Tissue Engineering
Shi, Z., Xu, Y., Mulatibieke, R., Zhong, Q., Pan, X., Chen, Y., Lian, Q., Luo, X., Shi, Z., & Zhu, Q. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s270681 · Cited by 41 · see citing works
Retracted
Effect of nano-hydroxyapatite on protein adsorption and cell adhesion of poly(lactic acid)/nano-hydroxyapatite composite microspheres
Wijerathne, H. M. C. S., Yan, D. M., Zeng, B., et al. (2020) · SN Applied Sciences · doi:10.1007/s42452-020-2531-8 · Cited by 40 · see citing works
Growth plate extracellular matrix-derived scaffolds for large bone defect healing
Cunniffe, G., Díaz‐Payno, P. J., Ramey, J., Mahon, O. R., Dunne, A., Thompson, E., O’Brien, F. J., & Kelly, D. J. (2017) · European Cells and Materials · doi:10.22203/ecm.v033a10 · Cited by 40 · see citing works
Self-citation
A two-phase and long-lasting multi-antibacterial coating enables titanium biomaterials to prevent implants-related infections
Lin, R., Wang, Z., Li, Z., et al. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100330 · Cited by 38 · see citing works
Synergistic use of biomaterials and licensed therapeutics to manipulate bone remodelling and promote non-union fracture repair
Simpson, C. R., Kelly, H. M., & Murphy, C. M. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.10.011 · Cited by 37 · see citing works
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Costard, L. S., Kelly, D. C., Power, R. N., Hobbs, C. J., Jaśkaniec, S., Nicolosi, V., Cavanagh, B., Curtin, C. M., & O’Brien, F. J. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12121219 · Cited by 37 · see citing works
Self-citation
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Power, R. N., Cavanagh, B., Dixon, J. E., Curtin, C. M., & O’Brien, F. J. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23031460 · Cited by 35 · see citing works
Self-citation
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Song, T., Zhao, F., Wang, Y., Li, D., Lei, N., Li, X., Xiao, Y., & Zhang, X. (2021) · Journal of Materials Chemistry B · doi:10.1039/d0tb02648d · Cited by 34 · see citing works
Synergistic Effects of Controlled-Released BMP-2 and VEGF from nHAC/PLGAs Scaffold on Osteogenesis
Wang, T., Guo, S., & Zhang, H. (2018) · BioMed Research International · doi:10.1155/2018/3516463 · Cited by 33 · see citing works
Enhancement of bone consolidation using high-frequency pulsed electromagnetic short-waves and titanium implants coated with biomimetic composite embedded into PLA matrix: in vivo evaluation
Oltean‐Dan, D., Dogaru, G. B., Tomoaia-Cotişel, M., Apostu, D., Meşter, A., Benea, H. R. C., Păiuşan, M. G., Jianu, M. E., Mocanu, A., Balint, R., et al. (2019) · International Journal of Nanomedicine · doi:10.2147/ijn.s205880 · Cited by 32 · see citing works
Loading of Antibiotic into Biocoated Hydroxyapatite Nanoparticles: Smart Antitumor Platforms with Regulated Release
Rivas, M., del Valle, L. J., Rodríguez-Rivero, A. M., et al. (2018) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.8b00353 · Cited by 31 · see citing works
3D printing collagen/heparin sulfate scaffolds boost neural network reconstruction and motor function recovery after traumatic brain injury in canine
Jiang, J., Liu, X., Chen, H., Dai, C., Niu, X., Dai, L., Chen, X., & Zhang, S. (2020) · Biomaterials Science · doi:10.1039/d0bm01116a · Cited by 27 · see citing works
A novel gene-activated matrix composed of PEI/plasmid-BMP2 complexes and hydroxyapatite/chitosan-microspheres promotes bone regeneration
Ding, R., Liu, Y., Cheng, D., Yang, G., Wu, W., Du, H., Jin, X., Chen, Y., Wang, Y., Heng, B. C., et al. (2022) · Nano Research · doi:10.1007/s12274-022-4292-8 · Cited by 26 · see citing works
Dual scaffold delivery of miR-210 mimic and miR-16 inhibitor enhances angiogenesis and osteogenesis to accelerate bone healing
Castaño, I. M., Raftery, R. M., Chen, G., Cavanagh, B., Quinn, B., Duffy, G. P., Curtin, C. M., & O’Brien, F. J. (2023) · Acta Biomaterialia · doi:10.1016/j.actbio.2023.09.049 · Cited by 26 · see citing works
Self-citation
Effects of nanocrystalline hydroxyapatite concentration and skeletal site on bone and cartilage formation in rats
Boller, L. A., Shiels, S. M., Florian, D. C., Peck, S. H., Schoenecker, J. G., Duvall, C. L., Wenke, J. C., & Guelcher, S. A. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.05.056 · Cited by 25 · see citing works
3D printing of injury-preconditioned secretome/collagen/heparan sulfate scaffolds for neurological recovery after traumatic brain injury in rats
Liu, X., Chang, Z., Chen, C., Liang, J., Shi, J., Xiu, F., Shao, Q., Meng, W.-W., Wang, J., & Li, X. (2022) · Stem Cell Research & Therapy · doi:10.1186/s13287-022-03208-0 · Cited by 26 · see citing works
Collagen-hydroxyapatite based scaffolds for bone trauma and regeneration: recent trends and future perspectives
Han, D., Wang, W., Gong, J., Ma, Y., & Li, Y. (2024) · Nanomedicine · doi:10.1080/17435889.2024.2375958 · Cited by 25 · see citing works
Advances in bioink-based 3D printed scaffolds: optimizing biocompatibility and mechanical properties for bone regeneration
Kumar, P., Sharma, J., Kumar, R., Najser, J., Frantík, J., Manuja, A., Sunnam, N., & Praveenkumar, S. (2025) · Biomaterials Science · doi:10.1039/d4bm01606h · Cited by 25 · see citing works
Magnesium Ion-Mediated Regulation of Osteogenesis and Osteoclastogenesis in 2D Culture and 3D Collagen/Nano-Hydroxyapatite Scaffolds for Enhanced Bone Repair
Paiva, S. S., Ferreira, A., Pakenham, E., Kaur, K., Cavanagh, B., O’Brien, F. J., & Murphy, C. M. (2025) · Journal of Functional Biomaterials · doi:10.3390/jfb16100363 · Cited by 23 · see citing works
Self-citation
Inflammatory-Driven Angiogenesis in Bone Augmentation with Bovine Hydroxyapatite, B-Tricalcium Phosphate, and Bioglasses: A Comparative Study
Anghelescu, V. M., Neculae, I. I., Dincă, O. M., Vlădan, C., Socoliuc, C. G., Cioplea, M. D., Nichita, L., Popp, C. G., Zurac, S., & Bucur, A. (2018) · Journal of Immunology Research · doi:10.1155/2018/9349207 · Cited by 22 · see citing works
Collagen, polycaprolactone and attapulgite composite scaffolds for in vivo bone repair in rabbit models
Zhao, H., Zhang, X., Zhou, D., Weng, Y., Qin, W., Pan, F., Lv, S., & Zhao, X. (2020) · Biomedical Materials · doi:10.1088/1748-605x/ab843f · Cited by 20 · see citing works
Role of HA and BG in engineering poly(ε‐caprolactone) porous scaffolds for accelerating cranial bone regeneration
Yin, H., Li, X., Wang, P., et al. (2018) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36584 · Cited by 19 · see citing works
Biomimetic hydroxyapatite/poly xylitol sebacic adibate/vitamin K nanocomposite for enhancing bone regeneration
Dai, Z., Dang, M., Zhang, W., Sumathra, M., Teh, S. W., & Pan, H. (2019) · Artificial Cells Nanomedicine and Biotechnology · doi:10.1080/21691401.2019.1573183 · Cited by 19 · see citing works
Hierarchical Collagen–Hydroxyapatite Nanostructures Designed through Layer-by-Layer Assembly of Crystal-Decorated Fibrils
Colaço, É., Brouri, D., Aissaoui, N., et al. (2019) · Biomacromolecules · doi:10.1021/acs.biomac.9b01299 · Cited by 18 · see citing works
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Li, X., Yin, H., Luo, E., Zhu, S., Wang, P., Zhang, Z., Liao, G., Xu, J., Li, Z., & Li, J. (2019) · ACS Applied Bio Materials · doi:10.1021/acsabm.9b00761 · Cited by 18 · see citing works
Wood-inspired highly anisotropic scaffold activated with in situ mineralized collagen for advanced bone regeneration
Wang, L., Chen, X., Guo, R., Wang, S., Hong-tao, Z., Chen, F., & Xiao, J. (2024) · Chemical Engineering Journal · doi:10.1016/j.cej.2024.153297 · Cited by 17 · see citing works
Biomaterials and stem cells targeting the microenvironment for traumatic brain injury repair: progress and prospects
Gao, Y., Tang, Z., Wu, Z., Zhang, J., Ma, J., Liu, S., & Wang, Y. (2025) · Biomaterials Science · doi:10.1039/d4bm01521e · Cited by 16 · see citing works
Accelerating bone healing in vivo by harnessing the age-altered activation of c-Jun N-terminal kinase 3
González‐Vázquez, A., Raftery, R. M., Günbay, S., Chen, G., Murray, D. J., & O’Brien, F. J. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120540 · Cited by 11 · see citing works
Self-citation
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Raftery, R. M., González‐Vázquez, A., Walsh, D. P., et al. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202401031 · Cited by 10 · see citing works
Self-citation
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Wang, Y., Lv, H., Ren, S., Zhang, J., Liu, X., Chen, S., Zhai, J., & Zhou, Y. (2024) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.4c00910 · Cited by 9 · see citing works
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McCormick, K., Herrero, J. M., Haas, H., Fattah, S., Heise, A., O’Brien, F. J., & Cryan, S. (2024) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.3c00898 · Cited by 10 · see citing works
Self-citation
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Zhu, R., Chen, Y., Ke, Q., Zhang, C., & Guo, Y. (2017) · Materials & Design · doi:10.1016/j.matdes.2017.03.008 · Cited by 8 · see citing works
Advances in tissue engineering strategies for periodontal and endodontic regeneration: Current therapies and future trends for disease treatment and tissue repair in the oral cavity
Sheehy, E. J., Coffey, N., Quigley, R. M., Duncan, H. F., Kennedy, O. D., & O’Brien, F. J. (2025) · Biomaterials · doi:10.1016/j.biomaterials.2025.123750 · Cited by 7 · see citing works
Self-citation
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Brady, R. T., O’Brien, F. J., & Hoey, D. A. (2022) · Bioengineering · doi:10.3390/bioengineering9010035 · Cited by 6 · see citing works
Self-citation
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He, H., Wang, L., Cai, X., Wei, N., Wang, Q., & Xiao, J. (2024) · Applied Materials Today · doi:10.1016/j.apmt.2024.102060 · Cited by 6 · see citing works
Collagen silver-doped hydroxyapatite scaffolds reinforced with 3D printed frameworks for infection prevention and enhanced repair of load-bearing bone defects
Genoud, K. J., Sadowska, J. M., Power, R. N., et al. (2025) · Biofabrication · doi:10.1088/1758-5090/adaf59 · Cited by 4 · see citing works
Self-citation
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Knudsen, E.-E., Dreyer, C. H., Overgaard, S., et al. (2025) · Calcified Tissue International · doi:10.1007/s00223-024-01309-x · Cited by 3 · see citing works
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Balint, R., Mocanu, A., Tomoaia, G., Riga, S., & Tomoaia-Cotişel, M. (2021) · Annals of the Academy of Romanian Scientists Series on Physics and Chemistry · doi:10.56082/annalsarsciphyschem.2021.2.53 · Cited by 3 · see citing works
Biomimetic short fiber reinforced 3-dimensional scaffold for bone tissue regeneration
Sun, L., Liu, M., Li, Y., et al. (2024) · Biomedical Materials · doi:10.1088/1748-605x/ad2405 · Cited by 3 · see citing works
Four-muscle surgery for infantile esotropia with V-pattern.
Kraft, S. P., & Scott, W. E. (1986) · PubMed · Cited by 3 · see citing works
Interface-engineered 3D-printed PCEC/collagen composite scaffold for large bone defect repair under static and mechanical stimulation
Liu, Y.-Y., Dobricic, M., Intini, C., O’Brien, F. J., LLorca, J., & Echeverry‐Rendón, M. (2025) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2025.115354 · Cited by 2 · see citing works
Self-citation
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Runzer, C. D. T., Sadowska, J. M., Roumans, N. J. T., Plank, C., Evans, C. H., O’Brien, F. J., van Griensven, M., & Balmayor, E. R. (2025) · Advanced Functional Materials · doi:10.1002/adfm.202508631 · Cited by 2 · see citing works
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An, L., Zhao, X., Xu, C., et al. (2019) · Russian Journal of Physical Chemistry A · doi:10.1134/s003602441907015x · Cited by 1 · see citing works
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Tavares, M. T., Farinha, J. P. S., Baleizão, C., & Mano, J. F. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-820508-2.00017-9 · Cited by 1 · see citing works
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Castaño, I. M., Raftery, R. M., Curtin, C. M., Grillari, J., Redl, H. R., & O’Brien, F. J. (2020) · Springer eBooks · doi:10.1007/978-3-319-08831-0_34 · Cited by 1 · see citing works
Self-citation
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Gonzalez‐Fernandez, T. (2018) · Arrow@dit (Dublin Institute of Technology)
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Rivas, M. (2019) · doi:10.5821/dissertation-2117-330133
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Self-citation
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Vaithilingam, V., Reys, L. L., Sthijns, M. M. J. P. E., Rademakers, T., de Vries, R. H. W., Mohammed, S. G., Bont, D. D., Jetten, M., de Koning, E. J. P., Mourad, N. I., et al. (2022) · Tissue Engineering Part A · doi:10.1089/ten.tea.2022.29025.abstracts
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He, H., Wang, L., Cai, X., Li, W., & Xiao, J. (2023) · Scientia Sinica Chimica · doi:10.1360/ssc-2023-0043
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Liu, X.-Y., Chang, Z.-H., Chen, C., Liang, J., Shi, J.-X., Fan, X., Shao, Q., Meng, W.-W., Wang, J.-J., & Li, X.-H. (2023) · Figshare · doi:10.6084/m9.figshare.22604758
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Cai, H., Liu, C., Song, W., Liu, Y., Qiao, A., & Du, T. (2026) · Journal of Materials Science · doi:10.1007/s10853-026-12464-x
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Colaço, É. (2019) · doi:10.70675/3a4a053dz9584z4861z9136z4a4dce09ff3c
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Meyer, M., Jonsdottir, H. R., Amado, I., Gonzalez, J. G., Bracken, S., Kaur, K., Hodgkinson, T., Murray, D. J., O'Brien, F. J., & González-Vázquez, A. (2026) · Bioengineering · doi:10.3390/bioengineering13070746
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Zhang, X., Wen, Y., Sun, Y., Hu, C., Li, X., Li, H., Fan, X., Liang, J., Chen, Y., & Fan, Y. (2026) · Chemical Engineering Journal · doi:10.1016/j.cej.2026.179192
Advanced functional biobased materials for stem cell-mediated regenerative therapy
Negi, S. S., Srivastava, U., Ahmad, F., et al. (2026) · Regenesis repair rehabilitation. · doi:10.1016/j.rerere.2026.07.004
Hybrid materials based on collagen-chitosan matrices modified by conjugates of silver nanoparticles with quercetin
Vasil’kov, A. Y., Voloshina, P. R., Naumkin, A. V., et al. (2026) · Russian Chemical Bulletin · doi:10.1007/s11172-026-5082-3

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Padmanabhan, S. K., Salvatore, L., Gervaso, F., Catalano, M., Taurino, A., Sannino, A., & Licciulli, A. (2014) · Journal of Nanoscience and Nanotechnology · doi:10.1166/jnn.2015.9489 · Cited by 45 · see citing works
Development of a nanoHydroxyapatite-collagen composite scaffold for bone tissue engineering
Cunniffe, G. M. (2026) · Research Portal (Queen's University Belfast) · doi:10.17034/32627433 · Cited by 1 · see citing works
Self-citation
Development and characterisation of a collagen nano-hydroxyapatite composite scaffold for bone tissue engineering
Cunniffe, G. M., Dickson, G. R., Partap, S., Stanton, K. T., & O’Brien, F. J. (2010) · Journal of Materials Science: Materials in Medicine · doi:10.1007/s10856-009-3964-1 · Cited by 199 · see citing works
Self-citation
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Kaviani, Z., & Zamanian, A. (2015) · Procedia Materials Science · doi:10.1016/j.mspro.2015.11.102 · Cited by 12 · see citing works
Effect of different hydroxyapatite incorporation methods on the structural and biological properties of porous collagen scaffolds for bone repair
Ryan, A. J., Gleeson, J. P., Matsiko, A., Thompson, E. M., & O’Brien, F. J. (2014) · Journal of Anatomy · doi:10.1111/joa.12262 · Cited by 65 · see citing works
Self-citation

Authors: Cunniffe, G. M., Curtin, C. M., Thompson, E. M., Dickson, G. R., & O’Brien, F. J.
Affiliations: Royal College of Surgeons in Ireland (IE)
Corresponding author: O’Brien, Fergal J.
ORCID: Cunniffe, Gráinne M.
Curtin, Caroline M.
Thompson, Emmet M.
O’Brien, Fergal J.
Venue: ACS Applied Materials & Interfaces
Publisher: American Chemical Society (ACS)
Published: 2016-08-30
Volume: 8
Issue: 36
Pages: 23477-23488
ISSN: 1944-8244 (ISSN-L)
1944-8252
DOI: 10.1021/acsami.6b06596
OpenAlex: W2512953699
Semantic Scholar: 4aa91e34e45b3be2b778d5e66b8b3f51f2e25ca7
Cited by: 95
References: 59
Citations by year: 2026: 5 · 2025: 8 · 2024: 7 · 2023: 7 · 2022: 8 · 2021: 6 · 2020: 15 · 2019: 15 · 2018: 10 · 2017: 13
FWCI: 6.11
Type: journal article
Language: en
Open access: closed
Locations: ACS Applied Materials & Interfaces (publishedVersion)
PubMed (publishedVersion)
Funders: European Research Council (239685)
Science Foundation Ireland (04/Yl1/B531, 06/RFP/ENM012, 11/RFP/ ENM/3063)
Topics: Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords: hydroxyapatite nanoparticles, collagen scaffolds, osteogenic response, osteoinduction, bone tissue engineering, composite scaffolds, Young's modulus, crosslinking, porosity, cell attachment, mineralization, critical-size calvarial defect, bone ingrowth
MeSH terms: Animals, Bone Regeneration, Collagen, Osteogenesis, Porosity, Durapatite, Tissue Engineering, Rats, Tissue Scaffolds, X-Ray Microtomography

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Content-Dependent Osteogenic Response of Nanohydroxyapatite: An in Vitro and in Vivo Assessment within Collagen-Based Scaffolds

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