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Cited by 12Skin Protection and AgingAdvancements in Transdermal Drug DeliveryMedicinal Plants and Neuroprotection

Centella asiatica extract‐SiO 2 nanocomposite: More than a drug‐delivery system for skin protection from oxidative damage

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Ebau, Federico, Scano, Alessandra, Manca, Maria Letizia, Manconi, Maria, Cabras, Valentina, Pilloni, Martina, Ennas, Guido·2023·Journal of Biomedical Materials Research Part A
DOI 10.1002/jbm.a.37462PMC PMC10092495
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Abstract

An innovative nanotechnology‐based approach was used for the preparation of Centella asiatica ( C. asiatica ) extract‐SiO 2 nanocomposites, specifically tailored for skin protection from oxidative damage. Different amounts of C. asiatica glycolic extract (1.0, 3.0, 5.0, and 10.0 wt %) and fumed silica were used to prepare the nanocomposites by means of ball milling method. The influence of both composition of the starting mixture and milling time on the final products was evaluated by different techniques such as X‐ray powder diffraction, scanning electron microscopy, infrared spectroscopy, thermogravimetric analysis, and nitrogen sorption analysis. Results confirmed the integrity of the natural extract after the milling process, and its successful loading in the silica matrix. No cytotoxicity was observed for the obtained nanocomposites, which showed high in‐vitro ability to scavenge 2,2‐diphenyl‐1‐picrylhydrazyl and to protect human keratinocytes from damages induced with hydrogen peroxide.

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  • Nanocomposites utilizing inorganic matrices such as porous silica or metal oxides are increasingly used to stabilize C. asiatica compounds and enable targeted dermal effects.

    “Nanocomposites utilizing inorganic matrices such as porous silica or metal oxides are increasingly used to stabilize C. asiatica compounds and enable targeted dermal effects.”

    From Centella asiatica (L.) Urb. in skin health and cosmeceuticals: mechanisms, clinical evidence, and advanced delivery systems (2025) · cites this paper as Ebau et al. 2023

    The Conclusions report that porous silica protects the C. asiatica extract, permits controlled release, and retains its antioxidant activity; the Introduction proposes the nanocomposite for skin damage linked to oxidative stress. The paper studies a silica matrix, not metal-oxide matrices, and does not establish that such nanocomposites are increasingly used or demonstrate targeted dermal effects in vivo. (LLM, conf=0.87)

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  • A silica-based nanocomposite preserved antioxidant activity and protected keratinocytes from oxidative stress.

    “Ebau et al. (2023) developed a silica-based nanocomposite that preserved antioxidant activity and protected keratinocytes from oxidative stress – an essential mechanism in anti-aging therapy.”

    From Centella asiatica (L.) Urb. in skin health and cosmeceuticals: mechanisms, clinical evidence, and advanced delivery systems (2025) · cites this paper as Ebau et al. 2023

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Research works printed but not linked: 34

  1. [1]Paocharoen V. The efficacy and side effects of oral Centella asiatica extract for wound healing promotion in diabetic wound patients. J Med Assoc Thai. 2010; 93(Suppl 7): S166‐ S170. 21298840
  2. [3]Shetty BS, Udupa SL, Udupa AL, Somayaji SN. Effect of Centella asiatica L (Umbelliferae) on Normal and dexamethasone‐suppressed wound healing in Wistar albino rats. Int J Low Extrem Wounds. 2006; 5: 137‐ 143. 16928669
  3. [4]Shukla A, Rasik AM, Jain GK, Shankar R, Kulshrestha DK, Dhawan BN. In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. J Ethnopharmacol. 1999; 65: 1‐ 11. 10350364
  4. [5]Maquart FX, Chastang F, Simeon A, Birembaut P, Gillery P, Wegrowski Y. Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental wounds. Eur J Dermatol. 1999; 9: 289‐ 296. 10356407
  5. [6]Chen YJ, Dai YS, Chen BF, et al. The effect of Tetrandrine and extracts of Centella asiatica on acute radiation dermatitis in rats. Biol Pharm Bull. 1999; 22: 703‐ 706. 10443466
  6. [7]Kosalwatna S, Shaipanich C, Bhanganada K. The effect of one percent centella asiatica cream on chronic ulcers. Siriraj Hosp Gaz. 1988; 40: 455‐ 460.
  7. [8]Camacho‐Alonso F, Torralba‐Ruiz MR, Garcia‐Carillo N, Lacal‐Lujan J, Martinez‐Diaz F, Sanchez‐Siles M. Effects of topical applications of porcine acellular urinary bladder matrix and Centella asiatica extract on oral wound healing in a rat model. Clin Oral Investig. 2019; 23(5): 2083‐ 2095. 30251055
  8. [9]Aziz HA, Taher M, Sulaiman WMAW, Susanti D, Chowdhury SR, Zakaria ZA. In vitro and in vivo wound healing studies of methanolic fraction of Centella asiatica extract. S Afr J Bot. 2017; 108: 163‐ 174.
  9. [10]Yao C‐H, Yeh J‐Y, Chen Y‐S, Li M‐H, Huang C‐H. Wound‐healing effect of electrospun gelatin nanofibres containingCentella asiaticaextract in a rat model. J Tissue Eng Regen Med. 2017; 11(3): 905‐ 915. 25711743
  10. [11]Ruszymah BH, Chowdhury SR, Manan NA, Fong OS, Adenan MI, Saim AB. Aqueous extract of Centella asiatica promotes corneal epithelium wound healing in vitro. J Ethnopharmacol. 2012; 140: 333‐ 338. 22301444
  11. [12]Somboonwong J, Kankaisre M, Tantisira B, Tantisira MH. Wound healing activities of different extracts of Centella asiatica in incision and burn wound models: an experimental animal study. BMC Compl Alternat Med. 2012; 12: 103. doi: 10.1186/1472-6882-12-103 PMC3492213 22817824 · DOI · Paper page
  12. [13]Brinkhaus B, Lindner M, Schuppan D, Hahn EG. Chemical, pharmacological and clinical profile of the east Asian medical plant Centella aslatica. Phytomedicine. 2000; 7: 427‐ 448. 11081995
  13. [14]Rosen H, Blumenthal A, McCallum J. Effect of asiaticoside on wound healing in the rat. Proc Soc Exp Biol Med. 1967; 125(1): 279‐ 80. doi: 10.3181/00379727-125-32070 6027541 · DOI
  14. [16]Podda E, Aragoni C, Arca M, et al. Morpholine‐ and Thiomorpholine‐Based Amidodithiophosphonato Nickel Complexes: Synthesis, Characterization, P–N Cleavage, Antibacterial Activity and Silica Nano Dispersion. J Nanosci Nanotechnol. 2021; 21: 2879‐ 2891. doi: 10.1166/JNN.2021.19058 33653454 · DOI
  15. [17]Scano A, Ebau F, Manca ML, et al. Novel Drug Delivery Systems for Natural Extracts: The Case Study of Vitis Vinifera Extract‐SiO2 Nanocomposites. Int J Pharm. 2018; 551: 84‐ 96. doi: 10.1016/j.ijpharm.2018.08.057 30194010 · DOI
  16. [18]Pilloni M, Ennas G, Casu M, et al. Drug silica nanocomposite: Preparation, characterization and skin permeation studies. Pharm Dev Technol. 2013; 18: 626‐ 633. doi: 10.3109/10837450.2011.653821 22324371 · DOI
  17. [19]Bylka W, Znajdek‐Awiżeń P, Studzińska‐Sroka E, Brzezińska M. Centella asiatica in cosmetology. Adv Dermatol Allergol. 2013; 30(46–9): 20. PMC3834700 24278045
  18. [20]Bylka W, Znajdek‐Awiżeń P, Studzińska‐Sroka E, Dańczak‐Pazdrowska A, Brzezińska M. Centella asiatica in dermatology: an overview. Phytother Res. 2014; 28: 1117‐ 1124. 24399761
  19. [21]Oberbaum M, Markovitis R, Weisman Z, Kalinkevitis A. Wound healing by homeopathic silica dilutions in mice. J Isr Med Assoc. 1992; 123: 79‐ 82. 1325402
  20. [22]Grotheer V, Goergens M, Fuchs PC, et al. The performance of an orthosilicic acid‐releasing silica gel fiber fleece in wound healing. Biomaterials. 2013; 34: 7314‐ 7327. doi: 10.1016/j.biomaterials.2013.06.012 23830471 · DOI
  21. [23]Tang F, Li L, Chen D. Cytotoxicity, in Vivo Skin Irritation and Acute Systemic Toxicity of the Mesoporous Magnesium Carbonate Upsalite®. Adv Mater. 2012; 24: 1504‐ 1534. doi: 10.1002/adma.201104763 22378538 · DOI
  22. [24]Scano A, Cabras V, Marongiu F, Peddis D, Pilloni M, Ennas G. New Opportunities in the Preparation of Nanocomposites for Biomedical Applications: Revised Mechanosynthesis of Magnetite‐Silica Nanocomposites. Mat Res Exp. 2017. doi: 10.1088/2053-1591/aa5867 · DOI
  23. [25]M. Thommes, K. Kaneko, A.V. Neimark, J.P. Olivier, F. Rodriguez‐Reinoso, J. Rouquerol, and K.S.W. Sing, IUPAC Technical Report, 2015, 87, 1051– 1069.
  24. [26]Rouquerol J, Rouquerol F, Llewellyn P, Maurinand G, Sing K. Adsorption by Powders and Porous Solids. 2nd ed. Academic Press Oxford (UK); 2013.
  25. [27]Manca ML, Peris JE, Melis V, et al. Nanoincorporation of curcumin in polymer‐glycerosomes and evaluation of their in vitro–in vivo suitability as pulmonary delivery systems. RSC Adv. 2015; 5: 105149‐ 105159.
  26. [28]Manca ML, Cencetti C, Matricardi P, et al. Glycerosomes: Use of hydrogenated soy phosphatidylcholine mixture and its effect on vesicle features and diclofenac skin penetration. Int J Pharm. 2016; 511(1): 198‐ 204. doi: 10.1016/j.ijpharm.2016.07.009 27418567 · DOI
  27. [29]Moulaoui K, Caddeo C, Manca ML, et al. Identification and nanoentrapment of polyphenolic phytocomplex from Fraxinus angustifolia: In vitro and in vivo wound healing potential. Eur J Med Chem. 2015; 89: 179‐ 188. doi: 10.1016/j.ejmech.2014.10.047 25462238 · DOI
  28. [30]Cano Sanchez M, Lancel S, Boulanger E, Neviere R. Targeting Oxidative Stress and Mitochondrial Dysfunction in the Treatment of Impaired Wound Healing: A Systematic Review. Antioxidants (Basel). 2018; 7(8): 98. doi: 10.3390/antiox7080098 30042332 PMC6115926 · DOI
  29. [31]Kohli SS, Kohli VS. Novel approaches for stability improvement in natural medicines. Pharmacogn Rev. 2011; 5(9): 48‐ 54. doi: 10.4103/0973 22096318 PMC3210007 · DOI
  30. [32]Nagalingam A. Drug delivery aspects of herbal medicines. Japanese Kampo Medicines for the Treatment of Common Diseases–Focus on Inflammation. Elsevier Inc; 2017. doi: 10.1016/B978-0-12-809398-6.00015-9 · DOI
  31. [33]Kumari A, Kumar V, Yadav SK. A Tool to Enhance Therapeutic Values of Natural Plant Products. Trends Med Res. 2012. doi: 10.3923/tmr.2012.34.42 · DOI
  32. [34]Sahil K, Akanksha M, Premjeet S, Bilandi A, Kapoor B. Microsphere: A review. Int J Res Pharm Chem. 2011; 1: 1184‐ 1198.
  33. [35]Chandu VP, Arunachalam A, Jeganath S, Yamini K, Tharangini K, Chaitanya G. Niosomes: A Novel Drug Delivery System. Int J Nov Trends Pharm Sci. 2012; 2(1): 25‐ 31.
  34. [36]Singh A, Saharan VA, Singh M, Bhandari A. Phytosome: drug delivery system for polyphenolic phytoconstituents. Iran J Pharm Sci. 2011; 7: 209‐ 220.

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Authors
Ebau, F., Scano, A., Manca, M. L., Manconi, M., Cabras, V., Pilloni, M., & Ennas, G.
Affiliations
  • University of Cagliari (IT)
  • Life and Environment Science Department, Section of Drug Sciences, CNBS University of Cagliari Cagliari Italy
Corresponding author
Scano, Alessandra
ORCID
Venue
Journal of Biomedical Materials Research Part A
Publisher
Wiley
Published
2022-10-24
Volume
111
Issue
3
Pages
300-308
ISSN
  • 1549-3296 (ISSN-L)
  • 1552-4965
DOI
10.1002/jbm.a.37462
PMC
PMC10092495
OpenAlex
W4307161876
Cited by
12
References
2
Citations by year
2026: 3 · 2025: 5 · 2024: 3 · 2022: 1
FWCI
2.20
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biomedical Materials Research Part A (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • UNICA IRIS Institutional Research Information System (University of Cagliari) (submittedVersion, other-oa)
Funders
  • Università degli Studi di Cagliari
  • Regione Autonoma della Sardegna
Topics
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
Keywords
Centella asiatica, glycolic extract, fumed silica, nanocomposites, drug delivery, skin protection, oxidative damage, antioxidant activity, DPPH radical scavenging, hydrogen peroxide, keratinocytes, cytotoxicity, ball milling
MeSH terms
Humans, Plant Extracts, Silicon Dioxide, Triterpenes, Drug Delivery Systems, Oxidative Stress, Centella, Nanocomposites

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