The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Effects of miR-335-5p in modulating osteogenic differentiation by specifically downregulating Wnt antagonist DKK1
Open accessAbstract
Dickkopf-related protein 1 (DKK1) is essential to maintain skeletal homeostasis as an inhibitor of Wnt signaling and osteogenic differentiation. The purpose of this study was to investigate the molecular mechanisms underlying the developmental stage–specific regulation of the DKK1 protein level. We performed a series of studies including luciferase reporter assays, micro-RNA microarray, site-specific mutations, and gain- and loss-of-function analyses. We found that the DKK1 protein level was regulated via DKK1 3' UTR by miRNA control, which was restricted to osteoblast-lineage cells. As a result of decreased DKK1 protein level by miR-335-5p, Wnt signaling was enhanced, as indicated by elevated GSK-3β phosphorylation and increased β-catenin transcriptional activity. The effects of miR-335-5p were reversed by anti-miR-335-5p treatment, which downregulated endogenous miR-335-5p. In vivo studies showed high expression levels of miR-335-5p in osteoblasts and hypertrophic chondrocytes of mouse embryos, indicating a pivotal role of miR-335-5p in regulating bone development. In conclusion, miR-335-5p activates Wnt signaling and promotes osteogenic differentiation by downregulating DKK1. This cell- and development-specific regulation is essential and mandatory for the initiation and progression of osteogenic differentiation. miR-335-5p proves to be a potential and useful targeting molecule for promoting bone formation and regeneration. © 2011 American Society for Bone and Mineral Research
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The functions of animal microRNAs
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High Bone Density Due to a Mutation in LDL-Receptor–Related Protein 5
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Wang, X., Guo, B., Li, Q., Peng, J., Yang, Z., Wang, A., Li, D., Hou, Z., Lv, K., Kan, G., et al. (2013) · Nature Medicine · doi:10.1038/nm.3026 · Cited by 559
Hassan, M. Q., Maeda, Y., Taipaleenmaki, H., Zhang, W., Jafferji, M., Gordon, J. A. R., Li, Z., Croce, C. M., van Wijnen, A. J., Stein, J. L., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.377515 · Cited by 297
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Overexpression of MiR-335-5p Promotes Bone Formation and Regeneration in Mice
Zhang, L., Tang, Y., Zhu, X., Tu, T., Sui, L., Han, Q., Yu, L., Meng, S., Zheng, L., Valverde, P., et al. (2017) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.3230 · Cited by 106
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Metadata
- Authors
- Zhang, J., Tu, Q., Bonewald, L. F., He, X., Stein, G., Lian, J., & Chen, J.
- Venue
- Journal of Bone and Mineral Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-02-23
- Volume
- 26
- Issue
- 8
- Pages
- 1953-1963
- ISSN
- 0884-0431 (ISSN-L)
- 1523-4681
- OpenAlex
- W2095004208
- Cited by
- 289
- References
- 9
- FWCI
- 5.89
- Type
- journal article
- Language
- en
- Open access
- green
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