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Hypoxia or in situ normoxia: The stem cell paradigm

Ivanovic, Zoran·2009·Journal of Cellular Physiology
DOI 10.1002/jcp.21690
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Abstract

Although O 2 concentrations are considerably lowered in vivo, depending on the tissue and cell population in question (some cells need almost anoxic environment for their maintenance) the cell and tissue cultures are usually performed at atmospheric O 2 concentration (20–21%). As an instructive example, the relationship between stem cells and micro‐environmental/culture oxygenation has been recapitulated. The basic principle of stem cell biology, “the generation‐age hypothesis,” and hypoxic metabolic properties of stem cells are considered in the context of the oxygen‐dependent evolution of life and its transposition to ontogenesis and development. A hypothesis relating the self‐renewal with the anaerobic and hypoxic metabolic properties of stem cells and the actual O 2 availability is elaborated (“oxygen stem cell paradigm”). Many examples demonstrated that the cellular response is substantially different at atmospheric O 2 concentration when compared to lower O 2 concentrations which better approximate the physiologic situation. These lower O 2 concentrations, traditionally called “hypoxia” represent, in fact, an in situ normoxia, and should be used in experimentation to get an insight of the real cell/cytokine physiology. The revision of our knowledge on cell/cytokine physiology, which has been acquired ex vivo at non physiological atmospheric (20–21%) O 2 concentrations representing a hyperoxic state for most primate cells, has thus become imperious. J. Cell. Physiol. 219: 271–275, 2009. © 2009 Wiley‐Liss, Inc.

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  • Reduced oxygen tension can have a significant impact on cell culture phenotype and is considered beneficial for the culture and differentiation of PSCs.

    “Reduced oxygen tension can have a significant impact on cell culture phenotype and is considered beneficial for the culture and differentiation of PSCs (Ivanovic, 2009; Jez et al., 2015; Pamies et al., 2013).”

    From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Ivanovic, 2009

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Authors
Ivanovic, Z.
Venue
Journal of Cellular Physiology
Publisher
Wiley
Published
2009-01-21
Volume
219
Issue
2
Pages
271-275
ISSN
  • 0021-9541
  • 1097-4652
DOI
10.1002/jcp.21690
Cited by
346
References
5
Language
en
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)

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