Cited by 90Redox biology and oxidative stressTelomeres, Telomerase, and SenescenceGenetics, Aging, and Longevity in Model Organisms

Reactive oxygen species control senescence-associated matrix metalloproteinase-1 through c-Jun-N-terminal kinase

Open access
Dasgupta, Jaya, Kar, Supriya, Liu, Rong, Joseph, Joy, Kalyanaraman, Balaraman, Remington, S. James, Chen, Ceshi, Melendez, J. Andres·2010·Journal of Cellular Physiology

Abstract

The lifetime exposure of organisms to oxidative stress influences many aging processes which involve the turnover of the extracellular matrix. In this study, we identify the redox‐responsive molecular signals that drive senescence‐associated (SA) matrix metalloproteinase‐1 (MMP‐1) expression. Precise biochemical monitoring revealed that senescent fibroblasts increase steady‐state (H 2 O 2) 3.5‐fold (13.7–48.6 pM) relative to young cells. Restricting H 2 O 2 production through low O 2 exposure or by antioxidant treatments prevented SA increases in MMP‐1 expression. The H 2 O 2‐dependent control of SA MMP‐1 is attributed to sustained JNK activation and c‐jun recruitment to the MMP‐1 promoter. SA JNK activation corresponds to increases and decreases in the levels of its activating kinase (MKK‐4) and inhibitory phosphatase (MKP‐1), respectively. Enforced MKP‐1 expression negates SA increases in JNK phosphorylation and MMP‐1 production. Overall, these studies define redox‐sensitive signaling networks regulating SA MMP‐1 expression and link the free radical theory of aging to initiation of aberrant matrix turnover. J. Cell. Physiol. 225: 52–62, 2010. © 2010 Wiley‐Liss, Inc.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1002/jcp.22193

Cites

44 of 44 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Dasgupta, J., Kar, S., Liu, R., Joseph, J., Kalyanaraman, B., Remington, S. J., Chen, C., & Melendez, J. A.
Affiliations
  • Albany Medical College (US)
  • Medical College of Wisconsin (US)
  • University of Oregon (US)
Corresponding author
Melendez, J. Andres
Venue
Journal of Cellular Physiology
Publisher
Wiley
Published
2010-07-15
Volume
225
Issue
1
Pages
52-62
ISSN
  • 0021-9541 (ISSN-L)
  • 1097-4652
OpenAlex
W2023237311
Cited by
90
References
44
Citations by year
2026: 5 · 2025: 3 · 2024: 5 · 2023: 6 · 2022: 8 · 2021: 10 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 4 · 2016: 7 · 2015: 7 · 2014: 4 · 2013: 8 · 2012: 8
FWCI
2.99
Type
journal article
Language
en
Open access
Locations
  • Journal of Cellular Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Philip Morris International
  • National Institute on Aging (AG031067, R01AG031067, AG031067-02S1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
Keywords
reactive oxygen species, c-Jun N-terminal kinase, oxidative stress, MKP-1, c-Jun, hydrogen peroxide, extracellular matrix turnover, free radical theory of aging, fibroblast senescence, antioxidant treatment, low oxygen exposure, redox signaling
MeSH terms
Cell Line, Fibroblasts, Humans, Hydrogen Peroxide, Metalloporphyrins, Oxidation-Reduction, Oxygen, Oxidants, Cellular Senescence, Reactive Oxygen Species, Oxidative Stress, Matrix Metalloproteinase 1, MAP Kinase Signaling System, JNK Mitogen-Activated Protein Kinases, Green Fluorescent Proteins, Dual Specificity Phosphatase 1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review