The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Direct analysis of drug candidates in tissue by matrix‐assisted laser desorption/ionization mass spectrometry
Abstract
Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) has been used to directly analyze and image pharmaceutical compounds in intact tissue. The anti‐tumor drug SCH 226374 was unambiguously determined in mouse tumor tissue using MALDI‐QqTOFMS (QSTAR) by monitoring the dissociation of the protonated drug at m/z 695.4 to its predominant fragment at m/z 228.1. A second drug, compound A, was detected in slices of rat brain tissue following oral administration with doses ranging from 1–25 mg/kg. Quantitation of compound A from whole brain homogenates using routine high‐performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) procedures revealed that concentrations of the drug in whole brain varied from a low of 24 ng/g to a high of 1790 ng/g. The drug candidate was successfully detected by MALDI‐QqTOF in samples from each dose, covering a range of approximately two orders of magnitude. In addition, good correlation was observed between the MALDI‐QqTOFMS intensities at each dose with the HPLC/MS/MS results. Thus the MALDI‐MS response is proportional to the amount of drug in tissue. Custom software was developed to facilitate the imaging of small molecules in tissue using the MALDI‐QqTOF mass spectrometer. Images revealing the spatial localization of SCH 226374 in tumor tissue and compound A in brain tissue were acquired. Copyright © 2003 John Wiley & Sons, Ltd.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular Imaging of Biological Samples: Localization of Peptides and Proteins Using MALDI-TOF MS
Caprioli, R. M., Farmer, T. B., & Gile, J. (1997) · Analytical Chemistry · doi:10.1021/ac970888i · Cited by 2,234
Self-citationMatrix-assisted ultraviolet laser desorption of non-volatile compounds
Karas, M., Bachmann, D., Bahr, U., & Hillenkamp, F. (1987) · International Journal of Mass Spectrometry and Ion Processes · doi:10.1016/0168-1176(87)87041-6 · Cited by 1,953
Stoeckli, M., Chaurand, P., Hallahan, D. E., & Caprioli, R. M. (2001) · Nature Medicine · doi:10.1038/86573 · Cited by 1,172
Self-citationDesorption–ionization mass spectrometry on porous silicon
Wei, J., Buriak, J. M., & Siuzdak, G. (1999) · Nature · doi:10.1038/20400 · Cited by 1,070
Porous Silicon as a Versatile Platform for Laser Desorption/Ionization Mass Spectrometry
Shen, Z., Thomas, J. J., Averbuj, C., Broo, K. M., Engelhard, M., Crowell, J. E., Finn, M. G., & Siuzdak, G. (2001) · Analytical Chemistry · doi:10.1021/ac000746f · Cited by 333
Liu, M., Bryant, M., Chen, J., Lee, S., Yaremko, B., Lipari, P., Malkowski, M. G., Ferrari, E., Nielsen, L. L., Prioli, N. A., et al. (1998) · PubMed · Cited by 309
Self-citationTodd, P. J., Schaaff, T. G., Chaurand, P., & Caprioli, R. M. (2001) · Journal of Mass Spectrometry · doi:10.1002/jms.153 · Cited by 298
Self-citationStoeckli, M., Farmer, T. B., & Caprioli, R. M. (1999) · Journal of the American Society for Mass Spectrometry · doi:10.1016/s1044-0305(98)00126-3 · Cited by 201
Self-citationClarke, F. C., Jamieson, M. J., Clark, D. A., Hammond, S. V., Jee, R. D., & Moffat, A. C. (2001) · Analytical Chemistry · doi:10.1021/ac001327l · Cited by 166
Peer Reviewed: Systematic LC/MS Metabolite Identification in Drug Discovery
Clarke, N. J., Rindgen, D., Korfmacher, W. A., & Cox, K. (2001) · Analytical Chemistry · doi:10.1021/ac012480y · Cited by 155
Self-citation
Cited by
McDonnell, L. A., & Heeren, R. M. A. (2007) · Mass Spectrometry Reviews · doi:10.1002/mas.20124 · Cited by 1,082
Advanced MALDI mass spectrometry imaging in pharmaceutical research and drug development
Schulz, S., Becker, M., Groseclose, M. R., Schadt, S., & Hopf, C. (2019) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2018.08.003 · Cited by 325
Mass spectrometry imaging for drug distribution studies
Prideaux, B., & Stoeckli, M. (2012) · Journal of Proteomics · doi:10.1016/j.jprot.2012.07.028 · Cited by 279
Proteomics in Diagnostic Pathology
Chaurand, P., Sanders, M. E., Jensen, R. A., & Caprioli, R. M. (2004) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)63367-6 · Cited by 253
Self-citationSumner, L. W., Lei, Z., Nikolau, B. J., & Saito, K. (2015) · Natural Product Reports · doi:10.1039/c4np00072b · Cited by 233
Metadata
- Authors
- Reyzer, M. L., Hsieh, Y., Ng, K., Korfmacher, W. A., & Caprioli, R. M.
- Venue
- Journal of Mass Spectrometry
- Publisher
- Wiley
- Published
- 2003-10
- Volume
- 38
- Issue
- 10
- Pages
- 1081-1092
- ISSN
- 1076-5174 (ISSN-L)
- 1096-9888
- OpenAlex
- W2150155174
- Cited by
- 371
- References
- 13
- FWCI
- 8.33
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Direct analysis of drug candidates in tissue by matrix‐assisted laser desorption/ionization mass spectrometry. https://app.cochat.ai/papers/10.1002/jms.525
- The paper
- Reyzer, M. L., Hsieh, Y., Ng, K., Korfmacher, W. A., & Caprioli, R. M. (2003). Direct analysis of drug candidates in tissue by matrix‐assisted laser desorption/ionization mass spectrometry. Journal of Mass Spectrometry. https://doi.org/10.1002/jms.525
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
