Preferential uptake and metabolism of docosahexaenoic acid in membrane phospholipids from rod and cone photoreceptor cells of human and monkey retinas
Abstract
The uptake, metabolism, and cellular distribution of [3H]docosahexaenoic acid (DHA) in human and monkey retinas were studied with biochemical and autoradiographic techniques. In specimens from two human retina biopsies, incubated for 4 hr or 6 hr with [3H]docosahexaenoic acid (110 nM), 80% of the esterified [3H]fatty acid was recovered in phospholipids and the remainder in triacylglycerols and diacylglycerols. The distribution of [3H]DHA in individual phospholipids (PL) was similar in both retinas, with phosphatidylcholine (PC) and phosphatidylethanolamine (PE) accounting for most of the label. A similar labeling profile was observed in glycerolipids from monkey retina, and after 1 hr of incubation, high labeling of phosphatidic acid (PA, 11%) and phosphatidylinositol (PI, 20%) was observed. In both human and monkey retinas, a preferential uptake of [3H]DHA by photoreceptor cells was revealed by autoradiography. Cone photoreceptors showed a slightly higher density of silver grains in their inner segments than did rod photoreceptors. Photoreceptors accounted for 59% and 79% of the total [3H]DHA taken up by the human and monkey retinas, respectively, the remainder being distributed throughout the neural retina. In conclusion, this study shows for the first time that in human and monkey retinas, DHA is taken up with a high degree of selectivity by photoreceptor cells, and then becomes esterified mainly into phospholipids that will be subsequently utilized for the synthesis of new disc membranes.
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Photoreceptor cells selectively take up DHA, which esterifies to phospholipids used for PRC disc membrane biogenesis.
“Photoreceptor cells (PRCs) selectively take up DHA, which esterifies to phospholipids used for PRC disc membrane biogenesis (Rodriguez de Turco et al., 1990).”
From Docosanoids and elovanoids from omega-3 fatty acids are pro-homeostatic modulators of inflammatory responses, cell damage and neuroprotection (2018) · cites this paper as Rodriguez de Turco et al., 1990
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Self-citation
- Authors
- de Turco, E. B. R., Gordon, W. C., Peyman, G. A., & Bazan, N. G.
- Affiliations
- Louisiana State University (US)
- Corresponding author
- Bazan, N. G.
- Venue
- Journal of Neuroscience Research
- Publisher
- Wiley
- Published
- 1990-12
- Volume
- 27
- Issue
- 4
- Pages
- 522-532
- ISSN
- 0360-4012 (ISSN-L)
- 1097-4547
- OpenAlex
- W1997858872
- Cited by
- 39
- References
- 37
- Citations by year
- 2026: 1 · 2025: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2018: 2 · 2017: 1 · 2014: 1 · 2012: 1
- FWCI
- 4.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neuroscience Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ey004428-07, 5r01ey007541-02)
- National Eye Institute (EY-02377, EY04428, P30EY002377, EY07541)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- docosahexaenoic acid, photoreceptors, rod photoreceptors, cone photoreceptors, fatty acid uptake, fatty acid esterification, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidic acid, triglycerides, diacylglycerol, human retina, monkey retina
- MeSH terms
- Aged, Aged, 80 and over, Animals, Autoradiography, Docosahexaenoic Acids, Female, Humans, Male, Membranes, Middle Aged, Phospholipids, Photoreceptor Cells, Rana pipiens, Retina, Saimiri, Species Specificity, In Vitro Techniques
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