Modification of characteristics of kefiran by changing the carbon source of Lactobacillus kefiranofaciens
Abstract
Abstract BACKGROUND: Kefiran is an exopolysaccharide that is widely used in the food and pharmaceutical industries. The aim of this study was to demonstrate that kefiran with different chemical structures can be obtained by changing the composition of the Lactobacillus kefiranofaciens fermentation medium. RESULTS: When using maltose as the sole carbon source: (1) the average molecular mass of kefiran was 1.5 × 105 Da, only 62.5% of that of the control (with lactose as the sole carbon source); (2) the glucose/galactose molar ratio of kefiran from the optimised medium was 1:10; (3) two variable‐angle CH bonds existed in the structure of kefiran; (4) the maximum viscosity of kefiran was [η] = 73.86 ± 5.3 dL g−1 and its specific rotatory power was [α]D = + 68°. Increasing the proportion of maltose in a mixed carbon source (maltose and lactose) from 20 to 100 g L−1 decreased the average molecular mass and glucose/galactose molar ratio of kefiran by 35 and 60% respectively. CONCLUSION: Kefiran with different chemical structures was obtained by changing the composition of the fermentation medium. The results presented here will facilitate further study of the relationship between the chemical structure and biological role of kefiran. Copyright © 2008 Society of Chemical Industry
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Organic nitrogen significantly improved the production of specific EPS, such as kefiran, while inorganic nitrogen leads to a lower amount of kefiran grains.
“Organic nitrogen (i.e., casein, tryptone, yeast extract, peptone) significantly improved the production of specific EPS, such as kefiran, while the use of inorganic nitrogen, such as ammonium chloride, leads to a lower amount of kefiran grains [61,62].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [61]
Cites
Effects of an exopolysaccharide (kefiran) on lipids, blood pressure, blood glucose, and constipation
Maeda, H., Zhu, X., Omura, K., Suzuki, S., & Kitamura, S. (2004) · BioFactors · doi:10.1002/biof.5520220141 · Cited by 167 · see citing works
Micheli, L., Uccelletti, D., Palleschi, C., & Crescenzi, V. (1999) · Applied Microbiology and Biotechnology · doi:10.1007/s002530051616 · Cited by 137 · see citing works
Enhanced kefiran production by mixed culture of and
Cheirsilp, B., Shimizu, H., & Shioya, S. (2002) · Journal of Biotechnology · doi:10.1016/s0168-1656(02)00228-6 · Cited by 125 · see citing works
ANTITUMOR ACTIVITY IN MICE OF ORALLY ADMINISTERED POLYSACCHARIDE FROM KEFIR GRAIN
SHIOMI, M., Sasaki, K., Murofushi, M., & AIBARA, K. (1982) · Japanese Journal of Medical Science and Biology · doi:10.7883/yoken1952.35.75 · Cited by 124 · see citing works
Rimada, P. S., & Abraham, A. G. (2003) · Le Lait · doi:10.1051/lait:2002051 · Cited by 121 · see citing works
Antitumoural antibacterial and antifungal activities of kefir and kefir grain
Çevikbaş, A., Yemni, E., Ezzedenn, F. W., Yardimici, T., Çevikbaş, U., & STOHS, S. J. (1994) · Phytotherapy Research · doi:10.1002/ptr.2650080205 · Cited by 115 · see citing works
The chemical structure of kefiran, the water-soluble polysaccharide of the kefir grain
Kooiman, P. (1968) · Carbohydrate Research · doi:10.1016/s0008-6215(00)81138-6 · Cited by 111 · see citing works
Cheirsilp, B., Shoji, H., Shimizu, H., & Shioya, S. (2003) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(03)80194-9 · Cited by 92 · see citing works
LIU, F., Liu, Y., Meng, Y., Yang, M., & HE, K. (2004) · Antiviral Research · doi:10.1016/j.antiviral.2004.04.006 · Cited by 64 · see citing works
Shioya, S., Cheirsilp, B., & Shimizu, H. (2001) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-001-0846-y · Cited by 45 · see citing works
Structural investigation of the capsular polysaccharide from Lactobacillus kefiranofaciens K1
Mukai, T., Toba, T., Itoh, T., & Adachi, S. (1990) · Carbohydrate Research · doi:10.1016/0008-6215(90)84039-w · Cited by 45 · see citing works
Yokoi, H., & Watanabe, T. (1992) · Journal of Fermentation and Bioengineering · doi:10.1016/0922-338x(92)90069-7 · Cited by 44 · see citing works
Andersson, U., & Rådström, P. (2002) · Environmental Microbiology · doi:10.1046/j.1462-2920.2002.00268.x · Cited by 37 · see citing works
Lactobacillus kefiranofaciens KF-75と酵母の混合培養によるケフィランの生産性向上
三枝, 隆., 立花, 國., & 藤尾, 雄. (1999) · Seibutsu Kogakkaishi/Seibutsu Kōgaku Kaishi · Cited by 3 · see citing works
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Wang, Y., Li, C., Liu, P., Ahmed, Z., Xiao, P., & Bai, X. (2010) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2010.06.013 · Cited by 407 · see citing works
Ahmed, Z., Wang, Y., Anjum, N., Ahmad, A., & Khan, S. T. (2013) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2012.06.009 · Cited by 236 · see citing works
Ryan, P. M., Ross, R. P., Fitzgerald, G. F., Caplice, N. M., & Stanton, C. (2015) · Food & Function · doi:10.1039/c4fo00529e · Cited by 221 · see citing works
Kefir micro-organisms: their role in grain assembly and health properties of fermented milk
Bengoa, A. A., Iraporda, C., Garrote, G. L., & Abraham, A. G. (2019) · Journal of Applied Microbiology · doi:10.1111/jam.14107 · Cited by 197 · see citing works
Jeong, D., Kim, D.-H., Kang, I.-B., Kim, H., Song, K.-Y., Kim, H.-S., & Seo, K.-H. (2017) · Food Control · doi:10.1016/j.foodcont.2017.02.033 · Cited by 198 · see citing works
Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
Prete, R., Alam, M. K., Perpetuini, G., Perla, C., Pittia, P., & Corsetti, A. (2021) · Foods · doi:10.3390/foods10071653 · Cited by 194 · see citing works
“, casein, tryptone, yeast extract, peptone) significantly improved the production of specific EPS, such as kefiran, while the use of inorganic nitrogen, such as ammonium chloride, leads to a lower amount of kefiran grains [61,62].”
BackgroundXu, Y., Cui, Y., Yue, F., Liu, L., Shan, Y., Liu, B., Zhou, Y., & Lü, X. (2019) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2019.03.032 · Cited by 189 · see citing works
Salazar, N., Prieto, A., Leal, J. A., Mayo, B., Bada-Gancedo, J. C., de los Reyes-Gavilán, C. G., & Ruas-Madiedo, P. (2009) · Journal of Dairy Science · doi:10.3168/jds.2009-2126 · Cited by 143 · see citing works
Zajšek, K., Goršek, A., & Kolar, M. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2012.11.142 · Cited by 83 · see citing works
- Authors
- Wang, M., & Bi, J.
- Affiliations
- Jiangnan University (CN)
- Corresponding author
- Wang, Miao
- ORCID
- Venue
- Journal of the Science of Food and Agriculture
- Publisher
- Wiley
- Published
- 2008-02-19
- Volume
- 88
- Issue
- 5
- Pages
- 763-769
- ISSN
- 0022-5142 (ISSN-L)
- 1097-0010
- OpenAlex
- W1999659298
- Cited by
- 34
- References
- 15
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 3 · 2021: 4 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 3 · 2016: 1 · 2015: 3 · 2014: 3 · 2013: 2 · 2012: 2
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the Science of Food and Agriculture (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- kefiran, Lactobacillus kefiranofaciens, exopolysaccharides, carbon source, maltose, lactose, molecular weight, viscosity, fermentation medium optimization, polysaccharide structure
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