S ‐Allylcysteine Ameliorates Aging Features via Regulating Mitochondrial Dynamics in Naturally Aged C57BL/6J Mice
Abstract
Scope: S ‐Allylcysteine (SAC) is the most abundant organosulfur molecule derived from aged garlic. The effects ofSAC on improving Aging in naturally aged C57BL/6J male mice and mitochondrial dynamics in Caenorhabditis elegans and its underlying mechanisms is evaluated. Methods and Results: When mice have attained reproductive senescence at 60 weeks of age, SAC is supplemented to 0.05% and 0.2% into their normal diet for 12 weeks. The results show that SAC could significantly improve the level of hepatic optic atrophy 1 ( OPA1 ) mRNA, which is a key factor for mitochondrial fusion, and consequently elevated the mitochondrial biogenesis‐related proteins sirtuin 1 (SIRT1) and peroxisome proliferator‐activated receptor‐gamma coactivator‐1 alpha (PGC‐1α), thus ameliorating oxidative stress, such as malondialdehyde (MDA) in the liver and 8‐hydroxy‐2'‐deoxyguanosine (8‐OHdG) in urine. Among the biochemical markers of aging, SAC significantly reduces liver galactosidase β1 (GLB1) and senescence‐associated β‐galactosidase (SA‐βgal), which are induced by replicative senescence. The mitochondria with green fluorescent protein (GFP)‐tagged transgenic strain SJ4103 C. elegans is incubated with 5 or 50 µM SAC, and SAC treated groups maintain the linear morphology of mitochondria. Conclusion: SAC regulates mitochondrial dynamics and ameliorated aging to a significant degree. This study also confirms that mitochondrial dynamics are a promising target for screening materials to combat aging and as a direction for anti‐aging product development.
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In vivo, 12-week dietary supplementation with S-allylcysteine (0.05% or 0.2%) to 60-week-old wild type mice reduces senescence, improves mitochondrial functions, and ameliorates both aging and OS biomarkers.
“In vivo, 12-week dietary supplementation with S-allylcysteine (0.05% or 0.2%) to 60-week-old wild type mice reduces senescence, improves mitochondrial functions, and ameliorates both aging and OS biomarkers [209].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [209]
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- Authors
- Chen, P., Chang, C., Lin, W., Nagabhushanam, K., Ho, C., & Pan, M.
- Affiliations
- Institute of Food Sciences and Technology National Taiwan University Taipei 10617 Taiwan
- National Taiwan University (TW)
- Sabinsa Corporation 20 Lake Drive, East Windsor NJ 08520 USA
- Rutgers, The State University of New Jersey (US)
- Asia University (TW)
- Corresponding author
- Pan, Min‐Hsiung
- Venue
- Molecular Nutrition & Food Research
- Publisher
- Wiley
- Published
- 2022-02-11
- Volume
- 66
- Issue
- 7
- Pages
- e2101077-e2101077
- ISSN
- 1613-4125 (ISSN-L)
- 1613-4133
- OpenAlex
- W4210488020
- Cited by
- 8
- References
- 68
- Citations by year
- 2026: 2 · 2024: 3 · 2023: 1 · 2022: 2
- FWCI
- 0.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Nutrition & Food Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Science and Technology, Taiwan (109‐2320‐B‐002 ‐012 ‐MY3, 110‐2320‐B‐002 ‐019 ‐MY3)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
- Keywords
- S-allylcysteine, garlic organosulfur compounds, aging, mitochondrial dynamics, mitochondrial fusion, OPA1, mitochondrial biogenesis, SIRT1, PGC-1α, oxidative stress, replicative senescence, senescence-associated β-galactosidase, Caenorhabditis elegans, anti-aging
- MeSH terms
- Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Aging, Animals, Cysteine, Male, Mice, Inbred C57BL, Caenorhabditis elegans, Mice, Sirtuin 1, Mitochondrial Dynamics
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