Metabolite profiling of fecal water extracts from human colorectal cancer
Open accessAbstract
Colorectal cancer is the second leading cause of cancer death in developed countries. There is a need for better preventive strategies to improve the outcome of this disease. The increasing availability of high-throughput methodologies opens up new possibilities for screening new markers. The application of NMR metabolic profiling to fecal water extracts has interesting potential as a diagnostic tool for detecting colorectal cancer. We obtained NMR metabolic profiles of fecal water extracts from patients with colorectal cancer and healthy individuals, to characterize possible differences between them and to identify potential diagnostic markers. Our results show that metabolic profiling of fecal water extracts is a cheap, reproducible and effective method for detecting colorectal cancer markers and therefore complements other stool-screening methods. A low concentration of short-chain fatty acids, such as acetate and butyrate, previously associated with the development of colorectal cancer, appears to be the most effective marker. Concentrations of proline and cysteine, which are major components of most colonic epithelium mucus glycoproteins, also display significant changes in samples from colorectal cancer. Differentiation between fecal water extracts from controls and patients with colorectal cancer by NMR spectroscopy combined with chemometric techniques opens up new possibilities for developing new, efficient, high-throughput screening protocols.
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A few cross-sectional studies reported that colorectal cancer patients had a lower abundance of butyrate-producing species and lower fecal butyrate levels than controls.
“a few cross-sectional studies reported that patients with CRC had a lower abundance of butyrate-producing species and lower fecal levels of butyrate than controls (Table 1).97, 133–135”
From Influence of the Gut Microbiome, Diet, and Environment on Risk of Colorectal Cancer (2020) · cites this paper as [135]
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- Authors
- Monleón, D., Morales, J. M., Barrasa, A., López, J. A., Vázquez, C., & Celda, B.
- Affiliations
- Universitat de València (ES)
- Fundación Instituto Valenciano de Oncología (ES)
- Corresponding author
- Monleón, Daniel
- Venue
- NMR in Biomedicine
- Publisher
- Wiley
- Published
- 2008-11-12
- Volume
- 22
- Issue
- 3
- Pages
- 342-348
- ISSN
- 0952-3480 (ISSN-L)
- 1099-1492
- OpenAlex
- W2011072368
- Cited by
- 186
- References
- 55
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 4 · 2023: 7 · 2022: 11 · 2021: 10 · 2020: 9 · 2019: 14 · 2018: 14 · 2017: 12 · 2016: 17 · 2015: 12 · 2014: 14 · 2013: 14 · 2012: 17
- FWCI
- 3.42
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- NMR in Biomedicine (publishedVersion)
- PubMed (publishedVersion)
- Repository of Digital Objects for Teaching Research and Culture (University of Valencia) (submittedVersion, cc-by-nc)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Physical Sciences › Engineering › Biomedical Engineering › Advanced Chemical Sensor Technologies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- colorectal cancer, NMR metabolomics, tumor markers, short-chain fatty acids, acetate, butyrate, proline, cysteine, cancer screening, chemometrics
- MeSH terms
- Adult, Aged, Feces, Female, Humans, Male, Middle Aged, Magnetic Resonance Spectroscopy, Water, Colorectal Neoplasms, Case-Control Studies, Principal Component Analysis, Complex Mixtures, Metabolomics
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