The DOI matches its Crossref record; 92 of the 92 references it cites were found in a registry or an index.
Difference gel electrophoresis
Abstract
DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS‐based methodologies and can circumvent their analytical limitations in areas such as intact protein analysis, (linear) detection over a wide range of protein abundances and, theoretically, applications where extreme sensitivity is needed. Thus, in quantitative proteomics DIGE is usually complementary to MS‐based quantitation and has some distinct advantages. This review describes the basics of DIGE and its unique properties and compares it to MS‐based methods in quantitative protein expression analysis.
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Difference gel electrophoresis. A single gel method for detecting changes in protein extracts
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Functional and quantitative proteomics using SILAC
Mann, M. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2067 · Cited by 931
Alban, A., David, S. O., Bjorkesten, L., Andersson, C., Sloge, E., Lewis, S., & Currie, I. (2003) · PROTEOMICS · doi:10.1002/pmic.200390006 · Cited by 924
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Encyclopedia of molecular cell biology and molecular medicine
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Correlation of mRNA and protein in complex biological samples
Maier, T., Güell, M., & Serrano, L. (2009) · FEBS Letters · doi:10.1016/j.febslet.2009.10.036 · Cited by 1,802
Kim, J. Y., Kim, Y.-G., & Lee, G. M. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3758-5 · Cited by 801
Integrative analysis of the heat shock response in Aspergillus fumigatus
Albrecht, D., Guthke, R., Brakhage, A. A., & Kniemeyer, O. (2010) · BMC Genomics · doi:10.1186/1471-2164-11-32 · Cited by 105
Use of Foodomics for Control of Food Processing and Assessing of Food Safety
Josić, D., Peršurić, Ž., Rešetar, D., Martinović, T., Saftić, L., & Kraljević Pavelić, S. (2017) · Advances in Food and Nutrition Research · doi:10.1016/bs.afnr.2016.12.001 · Cited by 28
Metadata
- Authors
- Timms, J. F., & Cramer, R.
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2008-12
- Volume
- 8
- Issue
- 23-24
- Pages
- 4886-4897
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- OpenAlex
- W2004254720
- Cited by
- 267
- References
- 92
- FWCI
- 14.67
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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