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Cited by 267

The DOI matches its Crossref record; 92 of the 92 references it cites were found in a registry or an index.

Difference gel electrophoresis

Timms, John F., Cramer, Rainer·2008·PROTEOMICS

Abstract

DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS‐based methodologies and can circumvent their analytical limitations in areas such as intact protein analysis, (linear) detection over a wide range of protein abundances and, theoretically, applications where extreme sensitivity is needed. Thus, in quantitative proteomics DIGE is usually complementary to MS‐based quantitation and has some distinct advantages. This review describes the basics of DIGE and its unique properties and compares it to MS‐based methods in quantitative protein expression analysis.

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Metadata

Authors
Timms, J. F., & Cramer, R.
Venue
PROTEOMICS
Publisher
Wiley
Published
2008-12
Volume
8
Issue
23-24
Pages
4886-4897
ISSN
  • 1615-9853 (ISSN-L)
  • 1615-9861
OpenAlex
W2004254720
Cited by
267
References
92
FWCI
14.67
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Difference gel electrophoresis. https://app.cochat.ai/papers/10.1002/pmic.200800298
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Timms, J. F., & Cramer, R. (2008). Difference gel electrophoresis. PROTEOMICS. https://doi.org/10.1002/pmic.200800298

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