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Cited by 7Mitochondrial Function and PathologyProtein Structure and DynamicsProtist diversity and phylogeny

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Ancestral sequence reconstruction of the Mic60 Mitofilin domain reveals residues supporting respiration in yeast

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Benning, Friederike M. C., Bell, Tristan A., Nguyen, Tran H., Syau, Della, Connell, Louise B., Liao, Yi‐Ting, Keating, Matthew P., Coughlin, Margaret, Nordstrom, Anja E. H., Ericsson, Maria, daCosta, Corrie J. B., Chao, Luke H.·2025·Protein Science
DOI 10.1002/pro.70207PMC PMC12183332

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Abstract

In eukaryotes, cellular respiration takes place in the cristae of mitochondria. The mitochondrial inner membrane protein Mic60, a core component of the mitochondrial contact site and cristae organizing system, is crucial for the organization and stabilization of crista junctions and its associated functions. While the C‐terminal Mitofilin domain of Mic60 is necessary for cellular respiration, the sequence determinants for this function have remained unclear. Here, we used ancestral sequence reconstruction to generate Mitofilin ancestors up to and including the last opisthokont common ancestor (LOCA). We found that yeast‐lineage derived Mitofilin ancestors as far back as the LOCA rescue respiration. By comparing Mitofilin ancestors, we identified four residues sufficient to explain the respiratory difference between yeast‐ and animal‐derived Mitofilin ancestors. Our results provide a foundation for investigating the conservation of Mic60‐mediated cristae junction interactions.

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  1. 10.1371/journal.pone.0160258 · DOI
  2. 10.1080/10635150600755453 · DOI · Paper page
  3. 10.1002/1873-3468.12384 · DOI
  4. 10.1007/S10005-001-0020-4 · DOI
  5. 10.1093/nar/gks1195 · DOI · Paper page
  6. 10.1126/sciadv.abo4946 · DOI
  7. 10.1091/mbc.e12-04-0295 · DOI
  8. Buchholz T‐O, 2019, Methods in cell biology, 277
  9. 10.1016/j.tibs.2016.01.001 · DOI · Paper page
  10. 10.1038/nature18015 · DOI
  11. 10.1016/S0022-5320(66)80027-8 · DOI
  12. 10.1093/gigascience/giab008 · DOI
  13. 10.1038/srep16064 · DOI
  14. 10.1093/jb/mvz111 · DOI
  15. 10.1002/pro.4472 · DOI
  16. 10.1093/nar/gkr367 · DOI · Paper page
  17. 10.2307/2412116 · DOI
  18. 10.1093/bioinformatics/btw069 · DOI
  19. 10.1016/S0968-0004(00)01609-1 · DOI
  20. 10.7554/eLife.07739 · DOI · Paper page
  21. 10.1093/nar/gkg563 · DOI
  22. 10.1385/1-59259-890-0:571 · DOI
  23. 10.1016/j.celrep.2016.11.049 · DOI
  24. 10.7554/eLife.06265 · DOI
  25. 10.1016/j.jsb.2016.06.007 · DOI
  26. 10.1038/emboj.2011.379 · DOI
  27. 10.1111/brv.13168 · DOI
  28. 10.1038/ncomms15258 · DOI
  29. 10.1146/annurev-biophys-070816-033631 · DOI
  30. 10.1083/jcb.201107053 · DOI
  31. 10.1109/MCSE.2007.55 · DOI · Paper page
  32. 10.1091/mbc.e04-08-0697 · DOI
  33. 10.1038/nmeth.4285 · DOI · Paper page
  34. 10.1093/nar/gkf436 · DOI · Paper page
  35. 10.1016/j.cub.2018.09.008 · DOI
  36. KhavnekarS ErdmannPS WanW.TOMOMAN: a software package for large scale cryo‐electron tomography data preprocessing community data sharing and collaborative computing. bioRxiv.2024. · DOI
  37. 10.1016/j.tcb.2020.08.008 · DOI
  38. 10.1091/mbc.E11-10-0831 · DOI
  39. 10.1093/molbev/msac174 · DOI
  40. Lamm L. SZufferey RDRighetto WWietrzynski KAYamauchi ABurt et al.MemBrain v2: an end‐to‐end tool for the analysis of membranes in cryo‐electron tomography. bioRxiv2024.https://doi.org/10.1101/2024.01.05.574336 · DOI
  41. 10.1093/nar/gki370 · DOI
  42. 10.1093/molbev/msn067 · DOI · Paper page
  43. 10.1007/s00284-019-01851-0 · DOI
  44. 10.1093/bioinformatics/btp324 · DOI · Paper page
  45. 10.1038/s41467-022-33957-8 · DOI
  46. 10.1007/978-1-62703-646-7_10 · DOI
  47. 10.1186/1471-2148-10-242 · DOI
  48. 10.1016/j.jsb.2005.07.007 · DOI
  49. 10.1186/1471-2105-7-339 · DOI
  50. 10.1111/2041-210X.12593 · DOI
  51. 10.1093/sysbio/syab010 · DOI
  52. 10.1093/molbev/msaa015 · DOI · Paper page
  53. 10.1038/s41592-022-01488-1 · DOI · Paper page
  54. 10.1016/j.cub.2023.02.059 · DOI
  55. 10.1016/j.cub.2015.04.006 · DOI
  56. Muñoz‐Gómez SA, 2017, The origin of mitochondrial cristae from Alphaproteobacteria, Mol Biol Evol, 34, 943
  57. 10.1128/MCB.06388-11 · DOI
  58. 10.1016/j.cub.2020.02.053 · DOI
  59. 10.1093/nar/gkac993 · DOI
  60. 10.1006/jsbi.1997.3885 · DOI
  61. 10.1146/annurev-physiol-021115-105011 · DOI
  62. 10.1002/pro.3943 · DOI
  63. 10.1016/j.str.2017.06.005 · DOI
  64. 10.1083/jcb.200811099 · DOI
  65. 10.3897/BDJ.5.e12581 · DOI
  66. 10.1016/j.jsb.2015.08.008 · DOI
  67. 10.1242/dmm.048912 · DOI
  68. 10.1242/jcs.201400 · DOI
  69. 10.1093/nar/gkab1112 · DOI · Paper page
  70. 10.1038/nmeth.2019 · DOI · Paper page
  71. 10.1073/pnas.1617220114 · DOI
  72. 10.1021/acssynbio.3c00423 · DOI
  73. 10.1093/molbev/mss140 · DOI
  74. 10.1038/s41556-018-0124-1 · DOI · Paper page
  75. 10.1093/molbev/msz228 · DOI
  76. 10.1038/s41418-019-0345-2 · DOI
  77. 10.1083/jcb.201609046 · DOI
  78. 10.1083/jcb.202003024 · DOI
  79. 10.1093/nar/gkac1052 · DOI · Paper page
  80. 10.3389/fnins.2018.00898 · DOI
  81. 10.1016/j.devcel.2011.08.026 · DOI
  82. 10.1016/j.jsb.2019.09.006 · DOI · Paper page
  83. 10.1007/BF00160154 · DOI · Paper page
  84. 10.1093/genetics/139.2.993 · DOI
  85. 10.1002/pro.4582 · DOI
  86. 10.1016/j.jmb.2012.05.004 · DOI
  87. 10.1016/j.jmb.2016.02.031 · DOI
  88. 10.1038/s44319-024-00336-x · DOI
  89. 10.1038/nmeth.4193 · DOI
  90. 10.1016/j.yjsbx.2022.100068 · DOI
  91. 10.1016/j.bbamcr.2008.06.013 · DOI

Metadata

Authors
Benning, F. M. C., Bell, T. A., Nguyen, T. H., Syau, D., Connell, L. B., Liao, Y., Keating, M. P., Coughlin, M., Nordstrom, A. E. H., Ericsson, M., daCosta, C. J. B., & Chao, L. H.
Affiliations
  • Harvard University (US)
  • Massachusetts General Hospital (US)
  • University of Ottawa (CA)
  • Boston University (US)
Corresponding author
  • Benning, Friederike M. C.
  • Chao, Luke H.
ORCID
Venue
Protein Science
Publisher
Wiley
Published
2025-06-22
Volume
34
Issue
7
Pages
e70207-e70207
ISSN
  • 0961-8368 (ISSN-L)
  • 1469-896X
DOI
10.1002/pro.70207
PMC
PMC12183332
OpenAlex
W4411531863
Cited by
7
References
3
Citations by year
2026: 4 · 2025: 3
FWCI
2.07
Type
journal article
Language
en
Open access
Licence
Locations
  • Protein Science (publishedVersion, cc-by-nc-nd)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion, cc-by-nc-nd)
Funders
  • Canadian Institutes of Health Research (377068)
  • National Institutes of Health (R35GM142553)
  • Simons Foundation (9736)
  • Gordon and Betty Moore Foundation
  • Canada Foundation for Innovation (34475)
  • Natural Sciences and Engineering Research Council of Canada (RGPIN‐2016‐04801)
  • Helen Hay Whitney Foundation
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (P180777)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Protist diversity and phylogeny
Keywords
Mic60, ancestral sequence reconstruction, mitochondrial cristae, cellular respiration, yeast, protein evolution, inner mitochondrial membrane

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