Cited by 57Nanoplatforms for cancer theranosticsLuminescence and Fluorescent MaterialsPhotodynamic Therapy Research Studies

Smart Probe for Tracing Cancer Therapy: Selective Cancer Cell Detection, Image-Guided Ablation, and Prediction of Therapeutic Response In Situ

Yuan, Youyong, Kwok, Ryan T. K., Tang, Ben Zhong, Liu, Bin·2015·Small

Abstract

Integrated diagnosis and therapy systems that can offer traceable cancer therapy are in high demand for personalized medicine. Herein, a pH-responsive polymeric probe containing tetraphenylsilole (TPS) with aggregation-induced emission characteristics and pheophorbide A (PheA) photosensitizer (PS) with aggregation-caused quenching property for tracing the whole process of cancer therapy is reported. At physiological conditions (pH 7.4), the probe self-assembles into nanoparticles (NPs), which show weak fluorescence of PheA with low phototoxicity, but strong green fluorescence from TPS for probe self-tracking. Upon uptake by cancer cells and entrapment in lysosomes (pH 5.0), the NPs disassemble to yield weak emission of TPS but strong red fluorescence of PheA with restored phototoxicity for PS activation monitoring. Upon light irradiation, the generated reactive oxygen species can cause lysosomal disruption to trigger cell apoptosis. Meanwhile, the probe leaks to the cytoplasm (pH 7.2), where the TPS fluorescence is restored for in situ visualization of the therapeutic response. The probe design thus represents a novel strategy for traceable cancer therapy.

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Crossref · 10.1002/smll.201501498

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Authors
Yuan, Y., Kwok, R. T. K., Tang, B. Z., & Liu, B.
Affiliations
  • National University of Singapore (SG)
  • Hong Kong University of Science and Technology (HK)
  • Agency for Science, Technology and Research (SG)
Corresponding author
  • Tang, Ben Zhong
  • Liu, Bin
Venue
Small
Publisher
Wiley
Published
2015-06-25
Volume
11
Issue
36
Pages
4682-4690
ISSN
1613-6810 (ISSN-L)
OpenAlex
W2164940176
Cited by
57
References
35
Citations by year
2025: 3 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 10 · 2020: 2 · 2019: 9 · 2018: 5 · 2017: 11 · 2016: 8
FWCI
3.98
Type
journal article
Language
en
Open access
closed
Locations
  • Small (publishedVersion)
  • PubMed (publishedVersion)
  • Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
  • National University of Singapore (submittedVersion)
Funders
  • Ministry of Defence (R279-000-340-232)
  • SMART (R279-000-378-592)
  • Singapore NRF Investigatorship and the Institute of Materials Research and Engineering of Singapore (IMRE/14-8P1110)
  • Research Grants Council of Hong Kong (604913, 16301614, N_HKUST620/11, N_HKUST604/14)
  • Guangdong Innovative Research Team Program (201101C0105067115)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
  • Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
Keywords
aggregation-induced emission, pheophorbide a, photosensitizers, pH-responsive probes, nanoparticle self-assembly, lysosomal pH, aggregation-caused quenching, reactive oxygen species, lysosomal disruption, apoptosis, cancer cell detection, image-guided ablation, treatment response monitoring, fluorescence imaging
MeSH terms
Chlorophyll, Cytoplasm, Cytosol, Fluorescent Dyes, Humans, Hydrogen-Ion Concentration, Lysosomes, Microscopy, Fluorescence, Neoplasms, Polylysine, Polymers, Apoptosis, Photosensitizing Agents, Reactive Oxygen Species, Cell Line, Tumor, Green Fluorescent Proteins, Nanoparticles, Precision Medicine, HEK293 Cells, MCF-7 Cells

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