Smart Probe for Tracing Cancer Therapy: Selective Cancer Cell Detection, Image-Guided Ablation, and Prediction of Therapeutic Response In Situ
Abstract
Integrated diagnosis and therapy systems that can offer traceable cancer therapy are in high demand for personalized medicine. Herein, a pH-responsive polymeric probe containing tetraphenylsilole (TPS) with aggregation-induced emission characteristics and pheophorbide A (PheA) photosensitizer (PS) with aggregation-caused quenching property for tracing the whole process of cancer therapy is reported. At physiological conditions (pH 7.4), the probe self-assembles into nanoparticles (NPs), which show weak fluorescence of PheA with low phototoxicity, but strong green fluorescence from TPS for probe self-tracking. Upon uptake by cancer cells and entrapment in lysosomes (pH 5.0), the NPs disassemble to yield weak emission of TPS but strong red fluorescence of PheA with restored phototoxicity for PS activation monitoring. Upon light irradiation, the generated reactive oxygen species can cause lysosomal disruption to trigger cell apoptosis. Meanwhile, the probe leaks to the cytoplasm (pH 7.2), where the TPS fluorescence is restored for in situ visualization of the therapeutic response. The probe design thus represents a novel strategy for traceable cancer therapy.
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- Authors
- Yuan, Y., Kwok, R. T. K., Tang, B. Z., & Liu, B.
- Affiliations
- National University of Singapore (SG)
- Hong Kong University of Science and Technology (HK)
- Agency for Science, Technology and Research (SG)
- Corresponding author
- Tang, Ben Zhong
- Liu, Bin
- Venue
- Small
- Publisher
- Wiley
- Published
- 2015-06-25
- Volume
- 11
- Issue
- 36
- Pages
- 4682-4690
- ISSN
- 1613-6810 (ISSN-L)
- OpenAlex
- W2164940176
- Cited by
- 57
- References
- 35
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 10 · 2020: 2 · 2019: 9 · 2018: 5 · 2017: 11 · 2016: 8
- FWCI
- 3.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Small (publishedVersion)
- PubMed (publishedVersion)
- Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
- National University of Singapore (submittedVersion)
- Funders
- Ministry of Defence (R279-000-340-232)
- SMART (R279-000-378-592)
- Singapore NRF Investigatorship and the Institute of Materials Research and Engineering of Singapore (IMRE/14-8P1110)
- Research Grants Council of Hong Kong (604913, 16301614, N_HKUST620/11, N_HKUST604/14)
- Guangdong Innovative Research Team Program (201101C0105067115)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Keywords
- aggregation-induced emission, pheophorbide a, photosensitizers, pH-responsive probes, nanoparticle self-assembly, lysosomal pH, aggregation-caused quenching, reactive oxygen species, lysosomal disruption, apoptosis, cancer cell detection, image-guided ablation, treatment response monitoring, fluorescence imaging
- MeSH terms
- Chlorophyll, Cytoplasm, Cytosol, Fluorescent Dyes, Humans, Hydrogen-Ion Concentration, Lysosomes, Microscopy, Fluorescence, Neoplasms, Polylysine, Polymers, Apoptosis, Photosensitizing Agents, Reactive Oxygen Species, Cell Line, Tumor, Green Fluorescent Proteins, Nanoparticles, Precision Medicine, HEK293 Cells, MCF-7 Cells
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