Biopebble Containers: DNA‐Directed Surface Assembly of Mesoporous Silica Nanoparticles for Cell Studies
Abstract
The development of methods for colloidal self‐assembly on solid surfaces is important for many applications in biomedical sciences. Toward this goal, described is a versatile class of mesoporous silica nanoparticles (MSN) that contain on their surface various types of DNA molecules to enable their self‐assembly into micropatterned surface architectures useful for cell studies. Monodisperse dye‐doped MSN are synthesized by biphase stratification and functionalized with an aptamer oligonucleotide that serves as gatekeeper for the triggered release of encapsulated molecular cargo, such as fluorescent dye rhodamine B or the anticancer drug doxorubicin. One or two additional types of oligonucleotides are installed on the MSN surface to enable DNA‐directed immobilization on solid substrates bearing patterns of complementary capture oligonucleotides. It is demonstrated that this strategy can be used for efficient self‐assembly of microstructured surface architectures, which not only promote the adhesion and guidance of cells but also are capable of affecting the fate of adhered cells through triggered release of their cargo. It is believed that this approach is useful for diverse applications in tissue engineering and nanobio sciences.
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Using hybridization assays with fluorescently-labelled probes, the authors estimated the number of barcodes available for hybridization on each file to be on the order of 10^8.
“Using hybridization assays with fluorescently-labelled probes35–37, we estimated the number of barcodes available for hybridization on each file to be on the order of 108 (Supplementary Fig. 7).”
From Random access DNA memory using Boolean search in an archival file storage system (2021) · cites this paper as [37]
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- Authors
- Sun, P., Leidner, A., Weigel, S., Weidler, P. G., Heissler, S., Scharnweber, T., & Niemeyer, C. M.
- Affiliations
- Karlsruhe Institute of Technology (DE)
- Corresponding author
- Niemeyer, Christof M.
- Venue
- Small
- Publisher
- Wiley
- Published
- 2019-04-15
- Volume
- 15
- Issue
- 20
- Pages
- e1900083-e1900083
- ISSN
- 1613-6810 (ISSN-L)
- 1613-6829
- OpenAlex
- W2940448258
- Cited by
- 21
- References
- 51
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 7 · 2020: 2 · 2019: 2
- FWCI
- 1.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Small (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (GRK 2039)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Materials Science › Materials Chemistry › Mesoporous Materials and Catalysis
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- mesoporous silica nanoparticles, DNA-directed self-assembly, aptamer functionalization, triggered release, doxorubicin delivery, rhodamine B, micropatterned surfaces, cell adhesion, cell guidance, cell fate, tissue engineering
- MeSH terms
- Colloids, DNA, Fluorescence, Fluorescent Dyes, Humans, Silicon Dioxide, Porosity, Nanoparticles, MCF-7 Cells
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