The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
Induction of Osteogenesis in Mesenchymal Stem Cells by Activated Monocytes/Macrophages Depends on Oncostatin M Signaling
Open accessAbstract
Bone resorption by osteoclasts and bone formation by osteoblasts are tightly coupled processes implicating factors in TNF, bone morphogenetic protein, and Wnt families. In osteoimmunology, macrophages were described as another critical cell population regulating bone formation by osteoblasts but the coupling factors were not identified. Using a high-throughput approach, we identified here Oncostatin M (OSM), a cytokine of the IL-6 family, as a major coupling factor produced by activated circulating CD14+ or bone marrow CD11b+ monocytes/macrophages that induce osteoblast differentiation and matrix mineralization from human mesenchymal stem cells while inhibiting adipogenesis. Upon activation of toll-like receptors (TLRs) by lipopolysaccharide or endogenous ligands, OSM was produced in classically activated inflammatory M1 and not M2 macrophages, through a cyclooxygenase-2 and prostaglandin-E2 regulatory loop. Stimulation of osteogenesis by activated monocytes/macrophages was prevented using neutralizing antibodies or siRNA to OSM, OSM receptor subunits gp130 and OSMR, or to the downstream transcription factor STAT3. The induced osteoblast differentiation program culminated with enhanced expression of CCAAT-enhancer-binding protein δ, Cbfa1, and alkaline phosphatase. Overexpression of OSM in the tibia of mice has led to new bone apposition with no sign of bone resorption. Two other cytokines have also a potent role in bone formation induced by monocytes/macrophages and activation of TLRs: IL-6 and leukemia inhibitory factor. We propose that during bone inflammation, infection, or injury, the IL-6 family signaling network activated by macrophages and TLR ligands stimulates bone formation that is largely uncoupled from bone resorption and is thus an important target for anabolic bone therapies. Disclosure of potential conflicts of interest is found at the end of this article.
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1 retracted
Takada, I., Mihara, M., Suzawa, M., Ohtake, F., Kobayashi, S., Igarashi, M., Youn, M.-Y., Takeyama, K.-I., Nakamura, T., Mezaki, Y., et al. (2007) · Nature Cell Biology · doi:10.1038/ncb1647 · Cited by 424
RetractedVandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., & Speleman, F. (2002) · Genome Biology · doi:10.1186/gb-2002-3-7-research0034 · Cited by 20,038
Macrophage Polarization in Bacterial Infections
Benoit, M., Desnues, B., & Mege, J.-L. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.181.6.3733 · Cited by 1,329
IL-6, RANKL, TNF-alpha/IL-1: interrelations in bone resorption pathophysiology
Steeve, K. T., Marc, P., Sandrine, T., Dominique, H., & Yannick, F. (2004) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2003.10.005 · Cited by 952
Chang, M. K., Raggatt, L.-J., Alexander, K. A., Kuliwaba, J. S., Fazzalari, N. L., Schroder, K., Maylin, E. R., Ripoll, V. M., Hume, D. A., & Pettit, A. R. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.181.2.1232 · Cited by 694
Theoleyre, S., Wittrant, Y., Tat, S. K., Fortun, Y., Redini, F., & Heymann, D. (2004) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2004.06.004 · Cited by 663
Self-citationWare, C. B., Horowitz, M. C., Renshaw, B. R., Hunt, J. S., Liggitt, D., Koblar, S. A., Gliniak, B. C., McKenna, H. J., Papayannopoulou, T., Thoma, B., et al. (1995) · Development · doi:10.1242/dev.121.5.1283 · Cited by 586
Zhang, X., Schwarz, E. M., Young, D. A., Puzas, J. E., Rosier, R. N., & O’Keefe, R. J. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0215681 · Cited by 577
Pederson, L., Ruan, M., Westendorf, J. J., Khosla, S., & Oursler, M. J. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0805133106 · Cited by 556
Chronically Inflamed Human Tissues Are Infiltrated by Highly Differentiated Th17 Lymphocytes
Pène, J., Chevalier, S., Preisser, L., Vénéreau, E., Guilleux, M.-H., Ghannam, S., Molès, J.-P., Danger, Y., Ravon, E., Lesaux, S., et al. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.180.11.7423 · Cited by 527
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Cited by
Osteoimmunomodulation for the development of advanced bone biomaterials
Chen, Z., Klein, T., Murray, R. Z., Crawford, R., Chang, J., Wu, C., & Xiao, Y. (2016) · Materials Today · doi:10.1016/j.mattod.2015.11.004 · Cited by 730
Sims, N. A., & Martin, T. J. (2014) · BoneKEy Reports · doi:10.1038/bonekey.2013.215 · Cited by 709
Kroner, A., Greenhalgh, A. D., Zarruk, J. G., Passos dos Santos, R., Gaestel, M., & David, S. (2014) · Neuron · doi:10.1016/j.neuron.2014.07.027 · Cited by 655
Zhu, G., Zhang, T., Chen, M., Yao, K., Huang, X., Zhang, B., Li, Y., Liu, J., Wang, Y., & Zhao, Z. (2021) · Bioactive Materials · doi:10.1016/j.bioactmat.2021.03.043 · Cited by 494
Current Advances in Immunomodulatory Biomaterials for Bone Regeneration
Lee, J., Byun, H., Madhurakkat Perikamana, S. K., Lee, S., & Shin, H. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201801106 · Cited by 452
Metadata
- Authors
- Guihard, P., Danger, Y., Brounais, B., David, E., Brion, R., Delecrin, J., Richards, C. D., Chevalier, S., Rédini, F., Heymann, D., Gascan, H., & Blanchard, F.
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-03-22
- Volume
- 30
- Issue
- 4
- Pages
- 762-772
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2020545615
- Cited by
- 476
- References
- 57
- FWCI
- 10.57
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Guihard, P., Danger, Y., Brounais, B., David, E., Brion, R., Delecrin, J., Richards, C. D., Chevalier, S., Rédini, F., Heymann, D., Gascan, H., & Blanchard, F. (2012). Induction of Osteogenesis in Mesenchymal Stem Cells by Activated Monocytes/Macrophages Depends on Oncostatin M Signaling. Stem Cells. https://doi.org/10.1002/stem.1040
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