The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
SIRT1 Positively Regulates Autophagy and Mitochondria Function in Embryonic Stem Cells Under Oxidative Stress
Open accessAbstract
SIRT1, an NAD-dependent deacetylase, plays a role in regulation of autophagy. SIRT1 increases mitochondrial function and reduces oxidative stress, and has been linked to age-related reactive oxygen species (ROS) generation, which is highly dependent on mitochondrial metabolism. H2O2 induces oxidative stress and autophagic cell death through interference with Beclin 1 and the mTOR signaling pathways. We evaluated connections between SIRT1 activity and induction of autophagy in murine (m) and human (h) embryonic stem cells (ESCs) upon ROS challenge. Exogenous H2 O2 (1 mM) induced apoptosis and autophagy in wild-type (WT) and Sirt1-/- mESCs. High concentrations of H2O2 (1 mM) induced more apoptosis in Sirt1-/-, than in WT mESCs. However, addition of 3-methyladenine, a widely used autophagy inhibitor, in combination with H2O2 induced more cell death in WT than in Sirt1-/- mESCs. Decreased induction of autophagy in Sirt1-/- mESCs was demonstrated by decreased conversion of LC3-I to LC3-II, lowered expression of Beclin-1, and decreased LC3 punctae and LysoTracker staining. H2O2 induced autophagy with loss of mitochondrial membrane potential and disruption of mitochondrial dynamics in Sirt1-/- mESCs. Increased phosphorylation of P70/85-S6 kinase and ribosomal S6 was noted in Sirt1-/- mESCs, suggesting that SIRT1 regulates the mTOR pathway. Consistent with effects in mESCs, inhibition of SIRT1 using Lentivirus-mediated SIRT1 shRNA in hESCs demonstrated that knockdown of SIRT1 decreased H2O2-induced autophagy. This suggests a role for SIRT1 in regulating autophagy and mitochondria function in ESCs upon oxidative stress, effects mediated at least in part by the class III PI3K/Beclin 1 and mTOR pathways.
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Citations
Cites
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AMPK regulates energy expenditure by modulating NAD+ metabolism and SIRT1 activity
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“ROS induces autophagy by inhibition of ATG4 activity [40].”
BackgroundSmall molecule activators of SIRT1 as therapeutics for the treatment of type 2 diabetes
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DRAM, a p53-Induced Modulator of Autophagy, Is Critical for Apoptosis
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Metabolic control of muscle mitochondrial function and fatty acid oxidation through SIRT1/PGC‐1α
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Cited by
Luen Tang, B. (2016) · Molecules and Cells · doi:10.14348/molcells.2016.2318 · Cited by 740
Stem cells and the impact of ROS signaling
Bigarella, C. L., Liang, R., & Ghaffari, S. (2014) · Development · doi:10.1242/dev.107086 · Cited by 632
Han, X., Tai, H., Wang, X., Wang, Z., Zhou, J., Wei, X., Ding, Y., Gong, H., Mo, C., Zhang, J., et al. (2016) · Aging Cell · doi:10.1111/acel.12446 · Cited by 302
Induction of autophagy supports the bioenergetic demands of quiescent muscle stem cell activation
Tang, A. H., & Rando, T. A. (2014) · The EMBO Journal · doi:10.15252/embj.201488278 · Cited by 294
Caloric restriction mimetics: towards a molecular definition
Madeo, F., Pietrocola, F., Eisenberg, T., & Kroemer, G. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4391 · Cited by 245
Metadata
- Authors
- Ou, X., Lee, M. R., Huang, X., Messina‐Graham, S., & Broxmeyer, H. E.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-04-17
- Volume
- 32
- Issue
- 5
- Pages
- 1183-1194
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W1535940973
- Cited by
- 336
- References
- 11
- FWCI
- 16.85
- Type
- journal article
- Language
- en
- Open access
- bronze
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