Cited by 72Stress Responses and CortisolTreatment of Major DepressionNeuroscience and Neuropharmacology Research

Cytoskeletal changes in the hippocampus following restraint stress. Role of serotonin and microtubules

Bianchi, Massimiliano, Heidbreder, Christian, Crespi, Francesco·2003·Synapse

Abstract

The aetiology of depression is associated with depletion in central levels of serotonin (5-HT). Hence, a major effect of antidepressant drugs is to increase synaptic 5-HT levels. Stressful conditions have also been shown to affect neuronal plasticity and 5-HT neurotransmission in the hippocampus. Neuronal plasticity, which is typically referred to as a structural adaptation of neurons to functional requirements, requires more dynamic forms of microtubules (cytoskeletal component). The alpha-tubulin, which is the major component of microtubules, can be postranslationally modified and both the tyrosinated (tyr-tub) and acetylated (acet-tub) forms are considered markers of more dynamic or more stable microtubules, respectively. The aim of the present work was to investigate the expression of tyr-tub and acet-tub in the hippocampus of rats submitted to either acute (6 h for 1 day) or sub-chronic (6 h for 4 days every day) restraint stress. In addition, ex vivo hippocampal 5-HT levels were monitored by differential pulse voltammetry to analyse the influence of both stress conditions upon 5-HT levels. Our results showed that the expression of tyr-tub in the hippocampus was significantly decreased to 70 +/- 7% following sub-chronic restraint stress (P < 0.01). In contrast, acute and sub-chronic restraint stress increased the hippocampal expression of acet-tub to 139 +/- 11% and 145 +/- 11% of control, respectively. Finally, 5-HT levels were significantly increased (P < 0.05) to 142 +/- 15% and 135 +/- 11% following acute and sub-chronic restraint stress, respectively. The stress-induced cytoskeletal changes observed in the present study suggest that the microtubular network is a potential new pathway that may increase our understanding of stress-related events.

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Crossref · 10.1002/syn.10230

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Authors
Bianchi, M., Heidbreder, C., & Crespi, F.
Affiliations
GlaxoSmithKline (Italy) (IT)
Corresponding author
Bianchi, Massimiliano
Venue
Synapse
Publisher
Wiley
Published
2003-05-23
Volume
49
Issue
3
Pages
188-194
ISSN
  • 0887-4476 (ISSN-L)
  • 1098-2396
OpenAlex
W1969328491
Cited by
72
References
53
Citations by year
2026: 3 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 1 · 2019: 1 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 5 · 2014: 5 · 2013: 1 · 2012: 2
FWCI
1.39
Type
journal article
Language
en
Open access
closed
Locations
  • Synapse (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
  • Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
restraint stress, hippocampus, serotonin, microtubules, alpha-tubulin, tyrosinated tubulin, acetylated tubulin, cytoskeleton, neuroplasticity, depression, chronic stress, acute stress, serotonin levels
MeSH terms
Animals, Cytoskeleton, Hippocampus, Male, Microtubules, Restraint, Physical, Serotonin, Stress, Physiological, Blotting, Western, Rats, Wistar, Rats

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