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A comparison study of different decellularization treatments on bovine articular cartilage
Open accessAbstract
Previous researches have emphasized on suitability of decellularized tissues for regenerative applications. The decellularization of cartilage tissue has always been a challenge as the final product must be balanced in both immunogenic residue and mechanical properties. This study was designed to compare and optimize the efficacy of the most common chemical decellularization treatments on articular cartilage. Freeze/thaw cycles, trypsin, ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), and Triton-X 100 were used at various concentrations and time durations for decellularization of bovine distal femoral joint cartilage samples. Histological staining, scanning electron microscopy, DNA quantification, compressive strength test, and Fourier-transform infrared spectroscopy were performed for evaluation of the decellularized cartilage samples. Treatment with 0.05% trypsin/EDTA for 1 day followed by 3% SDS for 2 days and 3% Triton X-100 for another 2 days resulted in significant reduction in DNA content and simultaneous maintenance of mechanical properties. Seeding the human adipose-derived stem cells onto the decellularized cartilage confirmed its biocompatibility. According to our findings, an optimized physiochemical decellularization method can yield in a nonimmunogenic biomechanically compatible decellularized tissue for cartilage regeneration application.
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“Although the application of trypsin in the initial steps as an adjuvant could enhance decellularization efficacy, the sole application of trypsin is insufficient to eliminate cells.”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [96]
In the citing paper
“Although the application of trypsin in the initial steps as an adjuvant could enhance decellularization efficacy, the sole application of trypsin is insufficient to eliminate cells.”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [96]
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Metadata
- Authors
- Ghassemi, T., Saghatoleslami, N., Mahdavi‐Shahri, N., Matin, M. M., Gheshlaghi, R., & Moradi, A.
- Affiliations
- Ferdowsi University of Mashhad (IR)
- Department of BiologyFerdowsi University of Mashhad (FUM) Mashhad Iran
- Mashhad University of Medical Sciences (IR)
- Corresponding author
- Moradi, Ali
- Venue
- Journal of Tissue Engineering and Regenerative Medicine
- Publisher
- Wiley
- Published
- 2019-08-27
- Volume
- 13
- Issue
- 10
- Pages
- 1861-1871
- ISSN
- 1932-6254 (ISSN-L)
- 1932-7005
- OpenAlex
- W2959992769
- Cited by
- 33
- References
- 51
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 6 · 2022: 9 · 2021: 7
- FWCI
- 3.82
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- other-oa
- onlinelibrary.wiley.com/termsAndConditions#vor (vor)
- doi.wiley.com/10.1002/tdm_license_1.1 (tdm)
- Locations
- Journal of Tissue Engineering and Regenerative Medicine (publishedVersion, other-oa)
- PubMed (publishedVersion)
- Funders
- Ferdowsi University of Mashhad (3/27432‐11/03/1392, #3/27432‐11/03/1392)
- Mashhad University of Medical Sciences (960363‐06/10/1396, #960363‐06/10/1396)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Keywords
- decellularization, articular cartilage, bovine cartilage, trypsin-EDTA, sodium dodecyl sulfate, Triton X-100, freeze-thaw cycles, DNA removal, compressive strength, cartilage biomechanical properties, adipose-derived stem cells, cartilage regeneration, immunogenicity
- MeSH terms
- Animals, Biocompatible Materials, Biomechanical Phenomena, Cartilage, Articular, Cattle, Collagen, DNA, Glycosaminoglycans, Water, Spectroscopy, Fourier Transform Infrared, Tissue Engineering
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