Cited by 40Nerve injury and regenerationTissue Engineering and Regenerative MedicineNeuroscience and Neural Engineering

Decellularized nerve matrix hydrogel and glial‐derived neurotrophic factor modifications assisted nerve repair with decellularized nerve matrix scaffolds

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Qiu, Shuai, Rao, Zilong, He, Fulin, Wang, Tao, Xu, Yiwei, Du, Zhaoyi, Yao, Zhi, Lin, Tao +4 more·2020·Journal of Tissue Engineering and Regenerative Medicine

Abstract

Nerve defects are challenging to address clinically without satisfactory treatments. As a reliable alternative to autografts, decellularized nerve matrix scaffolds (DNM-S) have been widely used in clinics for surgical nerve repair. However, DNM-S remain inferior to autografts in their ability to support nerve regeneration for long nerve defects. In this study, we systematically and clearly presented the nano-architecture of nerve-specific structures, including the endoneurium, basement membrane and perineurium/epineurium in DNM-S. Furthermore, we modified the DNM-S by supplementing decellularized nerve matrix hydrogel (DNMG) and glial-derived neurotrophic factor (GDNF) and then bridged a 50-mm sciatic nerve defect in a beagle model. Fifteen beagles were randomly divided into three groups (five per group): an autograft group, DNM-S group and GDNF-DNMG-modified DNM-S (DNM-S/GDNF@DNMG) group. DNM-S/GDNF@DNMG, as optimized nerve grafts, were used to bridge nerve defects in the same manner as in the DNM-S group. The repair outcome was evaluated by behavioural observations, electrophysiological assessments, regenerated nerve tissue histology and reinnervated target muscle examinations. Compared with the DNM-S group, limb function, electrophysiological responses and histological findings were improved in the DNM-S/GDNF@DNMG group 6 months after grafting, reflecting a narrower gap between the effects of DNM-S and autografts. In conclusion, modification of DNM-S with DNMG and GDNF enhanced nerve regeneration and functional recovery, indicating that noncellular modification of DNM-S is a promising method for treating long nerve defects.

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Crossref · 10.1002/term.3050

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Authors
Qiu, S., Rao, Z., He, F., Wang, T., Xu, Y., Du, Z., Yao, Z., Lin, T., Yan, L., Quan, D., Zhu, Q., & Liu, X.
Affiliations
  • Sun Yat-sen University (CN)
  • Department of Microsurgery, Orthopedic Trauma and Hand Surgery, The First Affiliated HospitalSun Yat‐sen University Guangzhou China
Corresponding author
  • Quan, Daping
  • Zhu, Qingtang
  • Liu, Xiaolin
Venue
Journal of Tissue Engineering and Regenerative Medicine
Publisher
Wiley
Published
2020-05-30
Volume
14
Issue
7
Pages
931-943
ISSN
  • 1932-6254 (ISSN-L)
  • 1932-7005
OpenAlex
W3020147187
Cited by
40
References
41
Citations by year
2026: 2 · 2025: 5 · 2024: 9 · 2023: 7 · 2022: 12 · 2021: 3 · 2020: 2
FWCI
2.75
Type
journal article
Language
en
Open access
Locations
  • Journal of Tissue Engineering and Regenerative Medicine (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Guangzhou Science and Technology Program key projects (201807070082, 201803010091, 201807010082)
  • National Natural Science Foundation of China (31670986)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
Keywords
nerve regeneration, glial cell line-derived neurotrophic factor, sciatic nerve defect, peripheral nerve repair, autograft alternative, perineurium, endoneurium, basement membrane, functional recovery
MeSH terms
Animals, Dogs, Extracellular Matrix, Male, Nerve Regeneration, Sciatic Nerve, Hydrogels, Glial Cell Line-Derived Neurotrophic Factor, Tissue Scaffolds

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