Cited by 36Carcinogens and Genotoxicity AssessmentToxic Organic Pollutants ImpactEnvironmental Toxicology and Ecotoxicology

Effects of UVA and visible light on the photogenotoxicity of benzo[a]pyrene and pyrene

Botta, Céline, Di Giorgio, Carole, Sabatier, Anne‐Sophie, De Méo, Michel·2009·Environmental Toxicology

Abstract

This study investigated the role of UVA/visible light (U, 320–800 nm) and visible light (V, 400–800 nm) in the phototoxicity and photogenotoxicity of two ubiquitous polycyclic aromatic hydrocarbons (PAH): benzo[a]pyrene (BaP) and Pyrene (Pyr). These mechanisms were evaluated by the WST‐1 test and the comet assay on normal human keratinocytes (NHK) and by the micronucleus test on CHO cells. The production of reactive oxygen species (ROS) was assessed through the induction of 8‐oxodeoxyguanine (8‐oxodG) lesions by immunofluorescence staining in NHK. Results of the WST‐1 test revealed the phototoxic properties of BaP and Pyr after irradiation with U and V lights. BaP presented the highest phototoxic properties. Results of the comet assay showed that U‐ and V‐irradiated BaP and Pyr induced increasing rates of DNA single‐strand breaks in NHK, in a dose dependent manner. The tested PAH could also induce increased levels of micronuclei in CHO cells after U and V irradiations. Increasing 8‐oxodG levels were detected after U and V irradiations in BaP‐ and Pyr‐treated keratinocytes and confirmed the involvement of ROS in the photogenotoxicity of PAH. Overall, this study highlighted the existence of an alternative pathway of PAH genotoxicity that is induced by UVA and/or visible light. Visible light is suggested to photoactivate PAH by a mechanism which is mainly based on oxidative reactions. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2009.

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Crossref · 10.1002/tox.20455

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  • In vitro experiments showed that the induction of cytotoxic and oxidative mechanisms by some PAHs was increased when human keratinocytes were exposed to both PAHs and UVA in particular.

    “Moreover, in vitro experiments showed that the induction of cytotoxic7, 8 and oxidative8, 52 mechanisms by some PAHs was increased when human keratinocytes were exposed to both PAHs and UVA in particular (for a review53).”

    From The impact of airborne pollution on skin (2019) · cites this paper as [52]

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Authors
Botta, C., Di Giorgio, C., Sabatier, A., & De Méo, M.
Affiliations
Aix-Marseille Université (FR)
Venue
Environmental Toxicology
Publisher
Wiley
Published
2008-11-10
Volume
24
Issue
5
Pages
492-505
ISSN
  • 1520-4081 (ISSN-L)
  • 1522-7278
OpenAlex
W2102725853
Cited by
36
References
68
Citations by year
2026: 1 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 1 · 2015: 1 · 2014: 5 · 2013: 2 · 2012: 2
FWCI
0.46
Type
journal article
Language
en
Open access
closed
Locations
  • Environmental Toxicology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
Keywords
benzo[a]pyrene, pyrene, polycyclic aromatic hydrocarbons, photogenotoxicity, UVA irradiation, visible light irradiation, phototoxicity, reactive oxygen species, single-strand breaks, micronuclei, keratinocytes, CHO cells, oxidative DNA damage
MeSH terms
8-Hydroxy-2'-Deoxyguanosine, Animals, Benzo(a)pyrene, Cell Line, Cells, Cultured, Cricetulus, Deoxyguanosine, DNA Damage, Environmental Pollutants, Cricetinae, Humans, Light, Mutagens, Pyrenes, Ultraviolet Rays, Keratinocytes, CHO Cells

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