Molecular cloning and tissue expression of a novel orphan G protein-coupled receptor from rat lung
Abstract
G protein-coupled receptors transduce the signal of a wide variety of hormones, neurotransmitters, cytokines, and other molecules across the cell membrane to elicit the corresponding response inside the target cells. We describe in this paper the molecular cloning and tissue distribution of a novel rat G protein-coupled receptor, GPR41, with highest homology to the human orphan G protein-coupled receptor DRY12. A lower degree of homology was seen with the receptors for bradykinin, angiotensin, and IL8. The expression of GPR41 appears to be the highest in brain and lung tissues, with lesser expression in heart, skeletal muscle, and kidney, as assayed by northern blotting. No GPR41 message was seen in spleen, liver, or testes. GPR41 failed to bind any of the ligands tested.
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SCFA receptors, mainly free fatty acid receptors and monocarboxylate transporters, have been discovered in the central and peripheral nervous systems, indicating direct signaling potential.
“Importantly, SCFA receptors, mainly free fatty acid receptors (349) and monocarboxylate transporters (350, 351), have been discovered in the central nervous system (CNS) and peripheral nervous system, indicating direct signaling potential.”
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- Authors
- Bonini, J. A., Anderson, S. M., & Steiner, D. F.
- Affiliations
- Howard Hughes Medical Institute (US)
- University of Chicago (US)
- Corresponding author
- Bonini, James A.
- ORCID
- Anderson, Spencer M.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1997-05
- Volume
- 234
- Issue
- 1
- Pages
- 190-193
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W1967990741
- Cited by
- 74
- References
- 13
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 5 · 2021: 6 · 2020: 4 · 2019: 5 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 3 · 2013: 3 · 2012: 3
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Keywords
- GPR41, orphan G protein-coupled receptor, rat lung, tissue expression, gene cloning, receptor homology, ligand binding, brain
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Gene Expression Regulation, Lung, Molecular Sequence Data, Receptors, Cell Surface, RNA, Messenger, Blotting, Northern, Signal Transduction, Antigens, CD, Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, Receptors, Interleukin, GTP-Binding Proteins, Receptors, Interleukin-8A, Receptors, G-Protein-Coupled, Rats
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