Functional Association between CBP and HNF4 inTrans-activation
Abstract
CBP functions as a key transcriptional coactivator for a variety of transcription factors. We show here that the hepatocyte nuclear factor 4 (HNF4), a transcription factor in the nuclear receptor superfamily with no defined ligand, is cloned by yeast two-hybrid system using CBP as a bait. GST-pull down assay with nuclear extracts or in vitro translation products revealed that CBP and HNF4 interact with each other at the middle portion (aa 119-375) of HNF4 and two distinct regions (aa 271-451 and 1626-2259) of CBP, respectively, in the ligand-independent manner. Co-transfection experiments indicated that CBP is capable of activating HNF4 site-mediated transcription. These results suggested a functional association between CBP and HNF4 in trans-activation.
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HNF4A directly interacts with CBP/p300.
“HNF4A was shown to directly interact with CBP/p300, which may account for its ability to regulate H3K27ac (2, 41, 42).”
From Hepatocyte Nuclear Factor 4‐Alpha Is Essential for the Active Epigenetic State at Enhancers in Mouse Liver (2019) · cites this paper as [41]
HNF4A was previously shown to recruit several coactivators such as glucocorticoid receptor interacting protein 1, steroid receptor coactivator1 and cAMP response element binding proteins to regulate gene expression.
“In addition to FOXA family members, HNF4A was previously shown to recruit several coactivators such as glucocorticoid receptor interacting protein 1, steroid receptor coactivator1 and cAMP response element binding proteins to regulate gene expression (41, 43).”
From Hepatocyte Nuclear Factor 4‐Alpha Is Essential for the Active Epigenetic State at Enhancers in Mouse Liver (2019) · cites this paper as [41]
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- Authors
- Yoshida, E., Aratani, S., Itou, H., Miyagishi, M., Takiguchi, M., Osumu, T., Murakami, K., & Fukamizu, A.
- Affiliations
- University of Tsukuba (JP)
- Kumamoto University (JP)
- Corresponding author
- Fukamizu, Akiyoshi
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1997-12
- Volume
- 241
- Issue
- 3
- Pages
- 664-669
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2016049443
- Cited by
- 104
- References
- 23
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 1 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 2.70
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (JSPSÐ RFTF 97L00804)
- Naito Foundation
- Ministry of Education, Culture, Sports, Science and Technology
- Asahi Glass Foundation
- Inamori Foundation
- Mochida Memorial Foundation for Medical and Pharmaceutical Research
- Uehara Memorial Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- CBP, HNF4, transcriptional coactivator, protein-protein interaction, transcriptional activation, nuclear receptor superfamily, yeast two-hybrid
- MeSH terms
- Animals, Cloning, Molecular, DNA-Binding Proteins, Liver, Nuclear Proteins, Phosphoproteins, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Transcription Factors, Transcription, Genetic, Transfection, Trans-Activators, CREB-Binding Protein, Mice, Rats, Hepatocyte Nuclear Factor 4, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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