Cited by 104Genomics and Chromatin DynamicsLiver physiology and pathologyEstrogen and related hormone effects

Functional Association between CBP and HNF4 inTrans-activation

Yoshida, Eisaku, Aratani, Satoko, Itou, Hiroshi, Miyagishi, Makoto, Takiguchi, Masaki, Osumu, Takashi, Murakami, Kazuo, Fukamizu, Akiyoshi·1997·Biochemical and Biophysical Research Communications

Abstract

CBP functions as a key transcriptional coactivator for a variety of transcription factors. We show here that the hepatocyte nuclear factor 4 (HNF4), a transcription factor in the nuclear receptor superfamily with no defined ligand, is cloned by yeast two-hybrid system using CBP as a bait. GST-pull down assay with nuclear extracts or in vitro translation products revealed that CBP and HNF4 interact with each other at the middle portion (aa 119-375) of HNF4 and two distinct regions (aa 271-451 and 1626-2259) of CBP, respectively, in the ligand-independent manner. Co-transfection experiments indicated that CBP is capable of activating HNF4 site-mediated transcription. These results suggested a functional association between CBP and HNF4 in trans-activation.

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Crossref · 10.1006/bbrc.1997.7871

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Authors
Yoshida, E., Aratani, S., Itou, H., Miyagishi, M., Takiguchi, M., Osumu, T., Murakami, K., & Fukamizu, A.
Affiliations
  • University of Tsukuba (JP)
  • Kumamoto University (JP)
Corresponding author
Fukamizu, Akiyoshi
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
1997-12
Volume
241
Issue
3
Pages
664-669
ISSN
0006-291X (ISSN-L)
OpenAlex
W2016049443
Cited by
104
References
23
Citations by year
2025: 1 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 1 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 2
FWCI
2.70
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Japan Society for the Promotion of Science (JSPSÐ RFTF 97L00804)
  • Naito Foundation
  • Ministry of Education, Culture, Sports, Science and Technology
  • Asahi Glass Foundation
  • Inamori Foundation
  • Mochida Memorial Foundation for Medical and Pharmaceutical Research
  • Uehara Memorial Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
CBP, HNF4, transcriptional coactivator, protein-protein interaction, transcriptional activation, nuclear receptor superfamily, yeast two-hybrid
MeSH terms
Animals, Cloning, Molecular, DNA-Binding Proteins, Liver, Nuclear Proteins, Phosphoproteins, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Transcription Factors, Transcription, Genetic, Transfection, Trans-Activators, CREB-Binding Protein, Mice, Rats, Hepatocyte Nuclear Factor 4, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors

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