Cited by 2,052Genomics and Chromatin DynamicsProtein Kinase Regulation and GTPase SignalingSignaling Pathways in Disease

Phosphorylated CREB binds specifically to the nuclear protein CBP

Chrivia, John C., Kwok, Roland P. S., Lamb, Ned, Hagiwara, Masatoshi, Montminy, Marc R., Goodman, Richard H.·1993·Nature

Abstract

Cyclic AMP-regulated gene expression frequently involves a DNA element known as the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. This modification stimulates interaction with one or more of the general transcription factors or, alternatively, allows recruitment of a co-activator. Here we report that CREB phosphorylated by protein kinase A binds specifically to a nuclear protein of M(r) 265K which we term CBP (for CREB-binding protein). Fusion of a heterologous DNA-binding domain to the amino terminus of CBP enables the chimaeric protein to function as a protein kinase A-regulated transcriptional activator. We propose that CBP may participate in cAMP-regulated gene expression by interacting with the activated phosphorylated form of CREB.

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Crossref · 10.1038/365855a0

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Authors
Chrivia, J. C., Kwok, R. P. S., Lamb, N., Hagiwara, M., Montminy, M. R., & Goodman, R. H.
Affiliations
  • Oregon Health & Science University (US)
  • Centre National de la Recherche Scientifique (FR)
  • Salk Institute for Biological Studies (US)
Venue
Nature
Published
1993-10
Volume
365
Issue
6449
Pages
855-859
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2033529039
Cited by
2,052
References
17
Citations by year
2026: 11 · 2025: 26 · 2024: 39 · 2023: 15 · 2022: 30 · 2021: 24 · 2020: 35 · 2019: 35 · 2018: 33 · 2017: 38 · 2016: 41 · 2015: 41 · 2014: 55 · 2013: 56 · 2012: 59
FWCI
21.69
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
Keywords
CREB, CREB-binding protein, CREB phosphorylation, protein kinase A, transcriptional coactivator, transcriptional activation, protein-protein interaction, chimeric transcription factors
MeSH terms
Cyclic AMP, Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Cell Line, Cloning, Molecular, DNA, Gene Expression Regulation, Humans, Molecular Sequence Data, Nuclear Proteins, Phosphorylation, Protein Binding, Recombinant Fusion Proteins, Thyroid Gland, Transcription Factors, Trans-Activators, Cyclic AMP Response Element-Binding Protein, Cyclic AMP-Dependent Protein Kinases, Genes, Reporter, CREB-Binding Protein, Mice

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