Cited by 107Bone Metabolism and DiseasesBone and Dental Protein StudiesOsteomyelitis and Bone Disorders Research

Osteoclastogenesis inhibitory factor (OCIF) directly inhibits bone-resorbing activity of isolated mature osteoclasts.

Hakeda, Yoshiyuki, Kobayashi, Yukinao, Yamaguchi, Kyoji, Yasuda, Hisataka, Tsuda, Eisuke, Higashio, Kanji, Miyata, Takashi, Kumegawa, Masayoshi·1998·Biochemical and Biophysical Research Communications

Abstract

Osteoclastogenesis inhibitory factor (OCIF) was previously reported to specifically inhibit osteoclast development by interrupting the action of osteoclast differentiation factor (ODF), which is expressed in stromal cells and plays an important role in osteoclastogenesis. Here we report the direct action of OCIF on isolated rabbit mature osteoclasts to inhibit their functional bone-resorbing activity. The cell population employed in this study consisted of mature osteoclasts with more than 95% of purity. The inhibition by OCIF was dose dependent and observed as early as 6 h after the OCIF addition. An OCIF-binding protein of 140 kDa was detected on the plasma membrane of osteoclasts. ODF with a Mr of 40 kDa was recently isolated as a ligand for OCIF and shows to be identical to TRANCE/RANKL. However, ODF was not detected in osteoclasts. OCIF did not have any impact on the mRNA levels of cathepsin K/OC2 and carbonic anhydrase II responsible for degradation of organic and inorganic bone matrices, respectively, or on osteoclast apoptosis. However, OCIF reduced or disrupted the formation of F-actin ring in isolated osteoclasts, the cytoskeletal structure of which is correlated with bone resorption. These findings demonstrate that OCIF directly inhibits osteoclast function through an ODF-independent mechanism besides blocking the generation of osteoclasts.

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Crossref · 10.1006/bbrc.1998.9523

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Authors
Hakeda, Y., Kobayashi, Y., Yamaguchi, K., Yasuda, H., Tsuda, E., Higashio, K., Miyata, T., & Kumegawa, M.
Affiliations
  • Meikai University (JP)
  • Megmilk Snow Brand (Japan) (JP)
Corresponding author
Hakeda, Yoshiyuki
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
1998-10
Volume
251
Issue
3
Pages
796-801
ISSN
0006-291X (ISSN-L)
OpenAlex
W2112216791
Cited by
107
References
27
Citations by year
2023: 2 · 2022: 1 · 2020: 1 · 2019: 2 · 2017: 1 · 2016: 4 · 2015: 2 · 2014: 1 · 2013: 4 · 2012: 2
FWCI
3.50
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
  • Health Sciences › Medicine › Rheumatology › Osteomyelitis and Bone Disorders Research
Keywords
osteoprotegerin, osteoclasts, bone resorption, osteoclast differentiation factor, F-actin ring, cathepsin K, carbonic anhydrase II, osteoclast apoptosis
MeSH terms
Actins, Animals, Bone Resorption, Carbonic Anhydrases, Cathepsins, Cell Separation, Dose-Response Relationship, Drug, Glycoproteins, Osteoclasts, Protein Binding, Rabbits, RNA, Messenger, Receptors, Tumor Necrosis Factor, Receptors, Cytoplasmic and Nuclear, Osteoprotegerin, Cathepsin K

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