Cited by 90Telomeres, Telomerase, and SenescenceLysosomal Storage Disorders ResearchCancer and biochemical research

Endogenous β-Galactosidase Activity in Continuously Nonproliferating Cells

Yegorov, Yegor E., Akimov, Sergey S., Hass, Ralf, Zelenin, Alexander V., Prudovsky, Igor A.·1998·Experimental Cell Research

Abstract

Cytochemically detectable activity of endogenous beta-galactosidase was found at pH 6.0 in Swiss 3T3 cells after long-term incubation in low serum or in the presence of heparin concentrations known to reversibly inhibit cell proliferation. A high percentage of beta-galactosidase-positive cells were detected in U937 and HL60 cultures at the late stage of macrophage-like differentiation induced by TPA. Interestingly, a small number of beta-galactosidase-positive cells were found even in the growing Swiss 3T3 cultures. These positive cells expressed morphological features similar to those of senescent cells. Thus, the activity of beta-galactosidase at pH 6.0 cannot be considered an exclusive marker of senescent cells since it is expressed in other types of nonproliferating cells.

Integrity signals

Registry

Crossref · 10.1006/excr.1998.4169

Cites

19 of 19 references verified0 citation errors · 0 not checkable

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  • Non-senescent macrophages may exhibit SA-β-gal positivity, and microglia can also stain positive in non-senescent contexts due to their phagocytic function and possible associated change in lysosomal numbers.

    “In particular, non-senescent macrophages may exhibit SA-β-gal positivity [145,146], and microglia can also stain positive in non-senescent contexts due to their phagocytic function and the possible associated change in lysosomal numbers [147].”

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Authors
Yegorov, Y. E., Akimov, S. S., Hass, R., Zelenin, A. V., & Prudovsky, I. A.
Affiliations
  • Engelhardt Institute of Molecular Biology (RU)
  • Humboldt-Universität zu Berlin (DE)
Corresponding author
Yegorov, Yegor E.
Venue
Experimental Cell Research
Publisher
Elsevier BV
Published
1998-08
Volume
243
Issue
1
Pages
207-211
ISSN
0014-4827 (ISSN-L)
OpenAlex
W2063754902
Cited by
90
References
18
Citations by year
2026: 3 · 2025: 4 · 2024: 10 · 2023: 8 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 5 · 2015: 3 · 2014: 9 · 2013: 6 · 2012: 3
FWCI
2.25
Type
journal article
Language
en
Open access
closed
Locations
  • Experimental Cell Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Health Sciences › Medicine › Physiology › Lysosomal Storage Disorders Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
Keywords
non-proliferating cells, cellular senescence, Swiss 3T3 cells, U937 cells, HL-60 cells, macrophage differentiation, phorbol ester, heparin, cytochemistry, pH 6.0
MeSH terms
Animals, beta-Galactosidase, Cell Differentiation, Cell Division, Cell Line, Fibroblasts, Hydrogen-Ion Concentration, 3T3 Cells, Cellular Senescence, Mice

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