Senescence-like phenotype in post-mitotic cells of mice entering middle age
Open accessAbstract
Staining mice tissues for β-galactosidase activity is a fundamental tool to detect age- or disease-associated cellular senescence. However, reported analyses of positivity for senescence-associated β-galactosidase activity or for other markers of senescence in post-mitotic cells of healthy murine tissues have been fragmentary or inconclusive. Here, we attempted to independently deepen this knowledge using multiple senescence markers within the same cells of wild type mice entering middle age (9 months of age). A histochemistry protocol for the pH-dependent detection of β-galactosidase activity in several tissues was used. At pH 6, routinely utilized to detect senescence-associated β-galactosidase activity, only specific cellular populations in the mouse body (including Purkinje cells and choroid plexus in the central nervous system) were detected as strongly positive for β-galactosidase activity. These post-mitotic cells were also positive for other established markers of senescence (p16, p21 and DPP4), detected by immunofluorescence, confirming a potential senescent phenotype. These data might contribute to understanding the functional relation between the senescence-associated β-galactosidase activity and senescence markers in post-mitotic cells in absence of disease or advanced aging.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A senescence-like phenotype is present in various post-mitotic cells of mice entering middle age in the absence of disease or advanced ageing.
“and a senescence-like phenotype is present in various post-mitotic cells of mice entering middle age in the absence of disease or advanced ageing.110”
From The costs and benefits of senotherapeutics for human health (2022) · cites this paper as [110]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
The serial cultivation of human diploid cell strains
Hayflick, L., & Moorhead, P. S. (1961) · Experimental Cell Research · doi:10.1016/0014-4827(61)90192-6 · Cited by 8,508 · see citing works
A biomarker that identifies senescent human cells in culture and in aging skin in vivo.
Dimri, G. P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E. E., Linskens, M., Rubelj, I., & Pereira-Smith, O. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.20.9363 · Cited by 7,477 · see citing works
Cellular Senescence: Defining a Path Forward
Gorgoulis, V., Adams, P. D., Alimonti, A., Bennett, D. C., Bischof, O., Bishop, C., Campisi, J., Collado, M., Evangelou, K., Ferbeyre, G., et al. (2019) · Cell · doi:10.1016/j.cell.2019.10.005 · Cited by 3,225 · cited 5× · see citing works
“Obesity or high fat diet results in the accumulation of senescent cells in the mouse brain [1, 20].”
BackgroundMethodInfluentialSenescence-associated β-galactosidase is lysosomal β-galactosidase.
Lee, B. Y., Han, J. A., Im, J. S., Morrone, A., Johung, K., Goodwin, E. C., Kleijer, W. J., DiMaio, D., & Hwang, E. S. (2006) · Aging Cell · doi:10.1111/j.1474-9726.2006.00199.x · Cited by 1,444 · see citing works
The intestinal crypt, a prototype stem cell compartment
Clevers, H. (2013) · Cell · doi:10.1016/j.cell.2013.07.004 · Cited by 1,223 · see citing works
Mouse Models in Aging Research
Flurkey, K., MCURRER, J., & HARRISON, D. (2007) · Elsevier eBooks · doi:10.1016/b978-012369454-6/50074-1 · Cited by 904 · see citing works
Chronic inflammation induces telomere dysfunction and accelerates ageing in mice
Jurk, D., Wilson, C., Passos, J. F., Oakley, F., Correia-Melo, C., Greaves, L., Saretzki, G., Fox, C., Lawless, C., Anderson, R., et al. (2014) · Nature Communications · doi:10.1038/ncomms5172 · Cited by 814 · see citing works
DNA damage response and cellular senescence in tissues of aging mice
Wang, C., Jurk, D., Maddick, M., Nelson, G., Martin‐Ruiz, C., & Von Zglinicki, T. (2009) · Aging Cell · doi:10.1111/j.1474-9726.2009.00481.x · Cited by 696 · see citing works
Jurk, D., Wang, C., Miwa, S., Maddick, M., Korolchuk, V., Tsolou, A., Gonos, E. S., Thrasivoulou, C., Jill Saffrey, M., Cameron, K., et al. (2012) · Aging Cell · doi:10.1111/j.1474-9726.2012.00870.x · Cited by 598 · see citing works
Tau protein aggregation is associated with cellular senescence in the brain
Musi, N., Valentine, J. M., Sickora, K. R., Baeuerle, E., Thompson, C. S., Shen, Q., & Orr, M. E. (2018) · Aging Cell · doi:10.1111/acel.12840 · Cited by 595 · see citing works
Interventions to slow aging in humans: are we ready?
Longo, V. D., Antebi, A., Bartke, A., Barzilai, N., Brown‐Borg, H. M., Caruso, C., Curiel, T. J., Cabo, R., Franceschi, C., Gems, D., et al. (2015) · Aging Cell · doi:10.1111/acel.12338 · Cited by 592 · see citing works
Self-citationObesity-Induced Cellular Senescence Drives Anxiety and Impairs Neurogenesis
Ogrodnik, M., Zhu, Y., Langhi, L. G. P., Tchkonia, T., Krüger, P., Fielder, E., Victorelli, S., Ruswhandi, R. A., Giorgadze, N., Pirtskhalava, T., et al. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2018.12.008 · Cited by 536 · see citing works
Methods to Detect Biomarkers of Cellular Senescence
Itahana, K., Campisi, J., & Dimri, G. P. (2007) · Methods in molecular biology · doi:10.1007/978-1-59745-361-5_3 · Cited by 520 · see citing works
Cellular senescence in brain aging and neurodegenerative diseases: evidence and perspectives
Baker, D. J., & Petersen, R. C. (2018) · Journal of Clinical Investigation · doi:10.1172/jci95145 · Cited by 514 · see citing works
Is β-Galactosidase Staining a Marker of Senescence in Vitro and in Vivo?
Severino, J., Allen, R. G., Balin, S., Balin, A., & Cristofalo, V. J. (2000) · Experimental Cell Research · doi:10.1006/excr.2000.4875 · Cited by 370 · see citing works
Identification of senescent cell surface targetable protein DPP4
Kim, K. M., Noh, J. H., Bodogai, M., Martindale, J. L., Yang, X., Indig, F. E., Basu, S. K., Ohnuma, K., Morimoto, C., Johnson, P. F., et al. (2017) · Genes & Development · doi:10.1101/gad.302570.117 · Cited by 270 · see citing works
Senescence-associated secretory phenotype contributes to pathological angiogenesis in retinopathy
Oubaha, M., Miloudi, K., Dejda, A., Guber, V., Mawambo, G., Germain, M.-A., Bourdel, G., Popovic, N., Rezende, F. A., Kaufman, R. J., et al. (2016) · Science Translational Medicine · doi:10.1126/scitranslmed.aaf9440 · Cited by 259 · see citing works
Choroid plexus ageing of the brain and Alzheimer s disease
Faure, G. C. (2003) · Frontiers in bioscience · doi:10.2741/1085 · Cited by 203 · see citing works
Cellular Senescence in Postmitotic Cells: Beyond Growth Arrest
Sapieha, P., & Mallette, F. A. (2018) · Trends in Cell Biology · doi:10.1016/j.tcb.2018.03.003 · Cited by 193 · see citing works
Survey of senescent cell markers with age in human tissues
Idda, M. L., McClusky, W. G., Lodde, V., Munk, R., Abdelmohsen, K., Rossi, M., & Gorospe, M. (2020) · Aging · doi:10.18632/aging.102903 · Cited by 188 · see citing works
Detection of Senescence-Associated Heterochromatin Foci (SAHF)
Aird, K. M., & Zhang, R. (2012) · Methods in molecular biology · doi:10.1007/978-1-62703-239-1_12 · Cited by 171 · see citing works
Senescence in Post-Mitotic Cells: A Driver of Aging?
von Zglinicki, T., Wan, T., & Miwa, S. (2021) · Antioxidants & Redox Signaling · doi:10.1089/ars.2020.8048 · Cited by 170 · see citing works
Chicas, A., Kapoor, A., Wang, X., Aksoy, O., Evertts, A. G., Zhang, M. Q., Garcia, B. A., Bernstein, E., & Lowe, S. W. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1119836109 · Cited by 161 · see citing works
Discovery, development, and future application of senolytics: theories and predictions
Wissler Gerdes, E. O., Zhu, Y., Tchkonia, T., & Kirkland, J. L. (2020) · The FEBS Journal · doi:10.1111/febs.15264 · Cited by 160 · see citing works
Leontieva, O. V., Demidenko, Z. N., & Blagosklonny, M. V. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1405723111 · Cited by 150 · see citing works
Biomarkers to identify and isolate senescent cells
Matjusaitis, M., Chin, G., Sarnoski, E. A., & Stolzing, A. (2016) · Ageing Research Reviews · doi:10.1016/j.arr.2016.05.003 · Cited by 144 · see citing works
Adult-onset, short-term dietary restriction reduces cell senescence in mice
Wang, C., Maddick, M., Miwa, S., Jurk, D., Czapiewski, R., Saretzki, G., Langie, S. A. S., Godschalk, R. W. L., Cameron, K., & von Zglinicki, T. (2010) · Aging · doi:10.18632/aging.100196 · Cited by 142 · see citing works
Kovačovicova, K. L., Školnaja, M., Heinmaa, M., Mistrík, M., Pata, P., Pata, I., Bártek, J., & Vinciguerra, M. (2018) · Frontiers in Oncology · doi:10.3389/fonc.2018.00459 · Cited by 110 · see citing works
Self-citationSA β Gal staining: Biomarker or delusion
Cristofalo, V. J. (2005) · Experimental Gerontology · doi:10.1016/j.exger.2005.08.005 · Cited by 92 · see citing works
Endogenous β-Galactosidase Activity in Continuously Nonproliferating Cells
Yegorov, Y. E., Akimov, S. S., Hass, R., Zelenin, A. V., & Prudovsky, I. A. (1998) · Experimental Cell Research · doi:10.1006/excr.1998.4169 · Cited by 90 · see citing works
Broad domains of histone H3 lysine 4 trimethylation in transcriptional regulation and disease
Park, S., Kim, G. W., Kwon, S. H., & Lee, J. (2020) · FEBS Journal · doi:10.1111/febs.15219 · Cited by 83 · see citing works
Histone macroH2A1.2 promotes metabolic health and leanness by inhibiting adipogenesis
Pazienza, V., Panebianco, C., Rappa, F., Memoli, D., Borghesan, M., Cannito, S., Oji, A., Mazza, G., Tamburrino, D., Fusai, G., et al. (2016) · Epigenetics & Chromatin · doi:10.1186/s13072-016-0098-9 · Cited by 78 · see citing works
Self-citationDPP-4 inhibitors: a promising therapeutic approach against Alzheimer’s disease
Angelopoulou, E., & Piperi, C. (2018) · Annals of Translational Medicine · doi:10.21037/atm.2018.04.41 · Cited by 73 · see citing works
The Genetic Program of Pancreatic β-Cell Replication In Vivo
Klochendler, A., Caspi, I., Weinberg-Corem, N., Moran, M., Friedlich, O., Elgavish, S., Nevo, Y., Helman, A., Gläser, B., Eden, A., et al. (2016) · Diabetes · doi:10.2337/db16-0003 · Cited by 71 · see citing works
Induction of cancer cell stemness by depletion of macrohistone H2A1 in hepatocellular carcinoma
Re, O. L., Fusilli, C., Rappa, F., Haele, M. V., Douet, J., Pindjáková, J., Rocha, S. W. S., Pata, I., Valčíková, B., Uldrijan, S., et al. (2017) · Hepatology · doi:10.1002/hep.29519 · Cited by 71 · see citing works
Self-citationTo help aging populations, classify organismal senescence
Calimport, S., Bentley, B. L., Stewart, C. E., Pawelec, G., Scuteri, A., Vinciguerra, M., Slack, C., Chen, D., Harries, L. W., Marchant, G., et al. (2019) · Science · doi:10.1126/science.aay7319 · Cited by 67 · see citing works
Self-citationRapamycin, proliferation and geroconversion to senescence
Blagosklonny, M. V. (2018) · Cell Cycle · doi:10.1080/15384101.2018.1554781 · Cited by 63 · see citing works
Re, O. L., Mazza, T., Giallongo, S., Sanna, P., Rappa, F., Luong, T. V., Volti, G. L., Drovakova, A., Roskams, T., Haele, M. V., et al. (2019) · Theranostics · doi:10.7150/thno.35045 · Cited by 45 · see citing works
Self-citationPhosphorylation of Histone H2AX in the Mouse Brain from Development to Senescence
Barral, S., Beltramo, R., Salio, C., Aimar, P., Lossi, L., & Merighi, A. (2014) · International Journal of Molecular Sciences · doi:10.3390/ijms15011554 · Cited by 38 · see citing works
Regulation of cyclin dependent kinase inhibitor proteins during neonatal cerebella development
Watanabe, G., Pena, P., Shambaugh, G. E., Haines, G. K., & Pestell, R. G. (1998) · Developmental Brain Research · doi:10.1016/s0165-3806(98)00032-7 · Cited by 38 · see citing works
Re, O. L., & Vinciguerra, M. (2017) · Genes · doi:10.3390/genes8120367 · Cited by 36 · see citing works
Self-citationCardioprotective mIGF-1/SIRT1 signaling induces hypertension, leukocytosis and fear response in mice
Bolasco, G., Calogero, R., Carrara, M., Banchaabouchi, M. A., Bilbao, D., Mazzoccoli, G., & Vinciguerra, M. (2012) · Aging · doi:10.18632/aging.100464 · Cited by 26 · see citing works
Self-citationBolon, B. (2008) · Toxicologic Pathology · doi:10.1177/0192623307312693 · Cited by 23 · see citing works
Isolation of senescent cells by iodixanol (OptiPrep) density gradient‐based separation
Kovačovicova, K. L., & Vinciguerra, M. (2019) · Cell Proliferation · doi:10.1111/cpr.12674 · Cited by 20 · see citing works
Self-citationThe discovery of β-galactosidase
Rouwenhorst, R. J., Pronk, J. T., & Dijken, J. P. V. (1989) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(89)90292-2 · Cited by 16 · see citing works
Merkwitz, C., Blaschuk, O. W., Schulz, A., & Ricken, A. M. (2016) · Journal of Histochemistry & Cytochemistry · doi:10.1369/0022155416665337 · Cited by 14 · see citing works
Endogenous beta-galactosidase expression in murine pancreatic islets
Inada, A., Nienaber, C. E., & Bonner‐Weir, S. (2006) · Diabetologia · doi:10.1007/s00125-006-0186-7 · Cited by 10 · see citing works
Embryonic and foetal expression patterns of the ciliopathy gene CEP164
Devlin, L. A., Ramsbottom, S. A., Overman, L. M., Lisgo, S., Clowry, G. J., Molinari, E., Powell, L., Miles, C., & Sayer, J. A. (2020) · PLoS ONE · doi:10.1371/journal.pone.0221914 · Cited by 9 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
The costs and benefits of senotherapeutics for human health
Raffaele, M., & Vinciguerra, M. (2022) · The Lancet Healthy Longevity · doi:10.1016/s2666-7568(21)00300-7 · Cited by 140 · see citing works
Self-citationde Mera‐Rodríguez, J. A., Álvarez‐Hernán, G., Gañán, Y., Martín‐Partido, G., Rodríguez‐León, J., & Francisco‐Morcillo, J. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.623175 · Cited by 123 · see citing works
A Blueprint for Characterizing Senescence
Roy, A. L., Sierra, F., Howcroft, K., Singer, D. S., Sharpless, N., Hodes, R. J., Wilder, E. L., & Anderson, J. M. (2020) · Cell · doi:10.1016/j.cell.2020.10.032 · Cited by 81 · see citing works
Muthamil, S., Kim, H., Jang, H., Lyu, J., Shin, U. C., Go, Y., Park, S., Lee, H. G., & Park, J. H. (2024) · Advanced Biology · doi:10.1002/adbi.202400079 · Cited by 79 · see citing works
Raffaele, M., Kovacovicova, K., Frohlich, J., Lo Re, O., Giallongo, S., Oben, J. A., Faldyna, M., Leva, L., Giannone, A. G., Cabibi, D., et al. (2021) · Cell Communication and Signaling · doi:10.1186/s12964-021-00731-0 · Cited by 60 · see citing works
Self-citationTargeting Immune Senescence in Atherosclerosis
Vellasamy, D. M., Lee, S.-J., Goh, K. W., Goh, B. H., Tang, Y.-Q., Ming, L. C., & Yap, W. H. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232113059 · Cited by 60 · see citing works
Subcellular structure, heterogeneity, and plasticity of senescent cells
Bitencourt, T. C., Vargas, J. E., Silva, A. O., Fraga, L. R., & Filippi‐Chiela, E. C. (2024) · Aging Cell · doi:10.1111/acel.14154 · Cited by 46 · see citing works
Cellular senescence and neurodegeneration
Holloway, K., Neherin, K., Dam, K. U., & Zhang, H. (2023) · Human Genetics · doi:10.1007/s00439-023-02565-x · Cited by 40 · see citing works
Ali, I., Zhang, H., Zaidi, S. A. A., & Zhou, G. (2024) · Ageing Research Reviews · doi:10.1016/j.arr.2024.102273 · Cited by 34 · see citing works
Giallongo, S., Řeháková, D., Biagini, T., Re, O. L., Raina, P., Lochmanová, G., Zdráhal, Z., Resnick, I. B., Pata, P., Pata, I., et al. (2022) · Stem Cells · doi:10.1093/stmcls/sxab004 · Cited by 30 · see citing works
Self-citationResearch progress on interferon and cellular senescence
Wang, D., Chen, K., Wang, Z., Wu, H., & Li, Y. (2024) · The FASEB Journal · doi:10.1096/fj.202400808rr · Cited by 23 · see citing works
A motor neuron disease mouse model reveals a non-canonical profile of senescence biomarkers
Torres, P., Anerillas, C., Ramírez‐Núñez, O., Fernàndez, A., Encinas, M., Povedano, M., Andrés‐Benito, P., Ferrer, I., Ayala, V., Pamplona, R., et al. (2022) · Disease Models & Mechanisms · doi:10.1242/dmm.049059 · Cited by 23 · see citing works
Podocyte injury at young age causes premature senescence and worsens glomerular aging
Pereira, B. M. V., Zeng, Y., Maggiore, J. C., Schweickart, R. A., Eng, D. G., Kaverina, N. V., McKinzie, S. R., Chang, A., Loretz, C. J., Thieme, K., et al. (2023) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00261.2023 · Cited by 22 · see citing works
Raffaele, M., Kovačovicova, K. L., Biagini, T., Re, O. L., Fröhlich, J., Giallongo, S., Nhan, J. D., Giannone, A. G., Cabibi, D., Ivanov, M. N., et al. (2021) · GeroScience · doi:10.1007/s11357-021-00487-y · Cited by 18 · see citing works
Self-citationWen, J., Bao, M., Tang, M., He, X., Yao, X., & Li, L. (2021) · Aging · doi:10.18632/aging.202907 · Cited by 17 · see citing works
Huang, H., Wang, T., Wang, L.-P., Huang, Y., Li, W., Wang, J., Hu, Y., & Zhou, Z. (2023) · Experimental Gerontology · doi:10.1016/j.exger.2023.112305 · Cited by 17 · see citing works
Kirchner, V. A., Badshah, J. S., Hong, S. K., Martinez, O. M., Pruett, T. L., & Niedernhofer, L. J. (2023) · Transplantation · doi:10.1097/tp.0000000000004838 · Cited by 15 · see citing works
Gudenschwager, C., Chavez, I., Cárdenas, C., & González-Billault, C. E. (2021) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2021/5586052 · Cited by 12 · see citing works
de Mera‐Rodríguez, J. A., Álvarez‐Hernán, G., Gañán, Y., Santos-Almeida, A., Martín‐Partido, G., Rodríguez‐León, J., & Francisco‐Morcillo, J. (2022) · Cells · doi:10.3390/cells11020298 · Cited by 10 · see citing works
Guzmán, T. J., Klöpper, N., Gurrola-Díaz, C. M., & Düfer, M. (2024) · Biogerontology (Dordrecht) · doi:10.1007/s10522-024-10107-9 · Cited by 9 · see citing works
de Mera‐Rodríguez, J. A., Álvarez‐Hernán, G., Gañán, Y., Solana-Fajardo, J., Martín‐Partido, G., Rodríguez‐León, J., & Francisco‐Morcillo, J. (2023) · PubMed · doi:10.14670/hh-18-549 · Cited by 4 · see citing works
Holloway, K., Neherin, K., Song, Y., Sato, K., Houston, A., Chen, F., Li, D., & Zhang, H. (2025) · Aging Cell · doi:10.1111/acel.14472 · Cited by 3 · see citing works
The Role of Telomeres in Senescence, Aging and Disease: Fiction and Reality
Saretzki, G. C. (2025) · OBM Geriatrics · doi:10.21926/obm.geriatr.2503324 · Cited by 3 · see citing works
Neherin, K., Holloway, K., Song, Y., Houston, A., Chen, F., Ding, L., & Zhang, H. (2026) · Advanced Biology · doi:10.1002/adbi.202500468 · Cited by 2 · see citing works
Accumulation of senescence observed in spinocerebellar ataxia type 7 mouse model
Miller, W., Pruett, C. L. H., Stone, W., Eide, C. R., Riddle, M. J., Popp, C. M., Yousefzadeh, M. J., Lees, C. J., Seelig, D. M., Thompson, E. L., et al. (2022) · PLoS ONE · doi:10.1371/journal.pone.0275580 · Cited by 1 · see citing works
Sexual Dimorphism in the Initial Apoptotic Switch During MASH Progression in Mice
Rajan, P. K., Sanabria, J., Schade, M. S., Udoh, U. S., Gorka, A., Sodhi, K., Pierre, S. V., & Sanabria, J. R. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27031501 · Cited by 1 · see citing works
DNA damage and histone variants
Giallongo, S., Re, O. L., & Vinciguerra, M. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-91081-1.00004-2 · Cited by 1 · see citing works
Self-citationRaffaele, M., Kovacovicova, K., Fröhlich, J., Lo Re, O., Giallongo, S., Oben, J. A., Faldyna, M., Levá, L., Giannone, A. G., Cabibi, D., et al. (2021) · Figshare · doi:10.6084/m9.figshare.14431610
Self-citation
- Authors
- Raffaele, M., Kovacovicova, K., Bonomini, F., Rezzani, R., Frohlich, J., & Vinciguerra, M.
- Affiliations
- University Hospital Brno (CZ)
- International Clinical Research Center (FNUSA-ICRC), St’ Anne University Hospital, Brno, Czech Republic
- University of Brescia (IT)
- Masaryk University (CZ)
- Venue
- Aging
- Publisher
- Impact Journals, LLC
- Published
- 2020-07-31
- Volume
- 12
- Issue
- 14
- Pages
- 13979-13990
- ISSN
- 1945-4589 (ISSN-L)
- PMC
- PMC7425512
- OpenAlex
- W3041036117
- Cited by
- 28
- References
- 48
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 5 · 2023: 5 · 2022: 7 · 2021: 6 · 2020: 1
- FWCI
- 2.86
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by
- Locations
- Aging (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Research Information System (Università degli Studi di Brescia) (submittedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Keywords
- cellular senescence, senescence-associated β-galactosidase, postmitotic cells, middle age (human life stage), p16, p21, dipeptidyl peptidase IV, Purkinje cells, choroid plexus, senescence markers, healthy aging, mice
- MeSH terms
- Aging, Animals, beta-Galactosidase, Hydrogen-Ion Concentration, Immunohistochemistry, Mice, Inbred C57BL, Mitosis, Biomarkers, Cellular Senescence, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
