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Cited by 261Neuroinflammation and Neurodegeneration MechanismsAlzheimer's disease research and treatmentsPhagocytosis and Immune Regulation

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Astrocytes Regulate Microglial Phagocytosis of Senile Plaque Cores of Alzheimer's Disease

DeWitt, David A., Perry, George, Cohen, Mark, Doller, Catherine, Silver, Jerry·1998·Experimental Neurology
DOI 10.1006/exnr.1997.6738
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Abstract

We have developed an in vitro model in which isolated senile plaque (SP) cores are presented to rat microglial cells in culture. Microglia rapidly phagocytosed, broke apart, and cleared SP cores. However, when cocultured with astrocytes, microglial phagocytosis was markedly suppressed, allowing the SPs to persist. Suppression of phagocytosis by astrocytes appears to be a general phenomena since microglia in the presence of astrocytes showed reduced capacity to phagocytose latex beads as well. The astrocyte effect on microglia is related in part to a diffusible factor(s) since astrocyte- but not fibroblast-conditioned media also reduced phagocytosis. These results suggest that while microglia have the capacity to phagocytose and remove SPs, astrocytes which lie in close association to microglia may help prevent the efficient clearance of SP material allowing them to persist in Alzheimer's disease.

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Metadata

Authors
DeWitt, D. A., Perry, G., Cohen, M., Doller, C., & Silver, J.
Affiliations
Case Western Reserve University (US)
ORCID
Venue
Experimental Neurology
Publisher
Elsevier BV
Published
1998-02
Volume
149
Issue
2
Pages
329-340
ISSN
0014-4886 (ISSN-L)
DOI
10.1006/exnr.1997.6738
OpenAlex
W2164956116
Cited by
261
References
69
Citations by year
2026: 2 · 2025: 3 · 2024: 6 · 2023: 11 · 2022: 6 · 2021: 12 · 2020: 3 · 2019: 7 · 2018: 8 · 2017: 6 · 2016: 12 · 2015: 13 · 2014: 11 · 2013: 8 · 2012: 13
FWCI
6.82
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Experimental Neurology (publishedVersion)
  • PubMed (publishedVersion)
  • Scholars Crossing (Liberty University) (submittedVersion)
Funders
  • National Institutes of Health (5r01ag009287-08)
  • National Institute on Aging (AG-00105-11A1, T32AG000105, AG 09287)
  • National Institute of Neurological Disorders and Stroke (NS 25713)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
  • Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
Keywords
astrocytes, microglia, phagocytosis, amyloid plaques, Alzheimer's disease, plaque clearance, astrocyte-microglia interaction, conditioned medium, diffusible factors, latex bead phagocytosis, fibroblasts
MeSH terms
Alzheimer Disease, Animals, Animals, Newborn, Astrocytes, Brain, Cells, Cultured, Cerebral Cortex, Frontal Lobe, Humans, Phagocytosis, Temporal Lobe, Rats, Sprague-Dawley, Microglia, Coculture Techniques, Rats, Plaque, Amyloid

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