CoChat
Sign Up
Cited by 261Neuroinflammation and Neurodegeneration MechanismsAlzheimer's disease research and treatmentsPhagocytosis and Immune Regulation

The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Astrocytes Regulate Microglial Phagocytosis of Senile Plaque Cores of Alzheimer's Disease

DeWitt, David A., Perry, George, Cohen, Mark, Doller, Catherine, Silver, Jerry·1998·Experimental Neurology
DOI 10.1006/exnr.1997.6738
Open this paper in CoChat — free account

Abstract

We have developed an in vitro model in which isolated senile plaque (SP) cores are presented to rat microglial cells in culture. Microglia rapidly phagocytosed, broke apart, and cleared SP cores. However, when cocultured with astrocytes, microglial phagocytosis was markedly suppressed, allowing the SPs to persist. Suppression of phagocytosis by astrocytes appears to be a general phenomena since microglia in the presence of astrocytes showed reduced capacity to phagocytose latex beads as well. The astrocyte effect on microglia is related in part to a diffusible factor(s) since astrocyte- but not fibroblast-conditioned media also reduced phagocytosis. These results suggest that while microglia have the capacity to phagocytose and remove SPs, astrocytes which lie in close association to microglia may help prevent the efficient clearance of SP material allowing them to persist in Alzheimer's disease.

Overview

Integrity signals

Registry

Crossref · 10.1006/exnr.1997.6738

Cites

0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Show all 4 references

Research works printed but not linked: 65

  1. Abd-El-Basset, 1995, Effect of bacterial wall lipopolysaccharide (LPS) on morphology, motility and cytoskeletal organization of microglia in cultures, J. Neurosci. Res., 41, 222, 10.1002/jnr.490410210 · DOI
  2. Araujo, 1992, β-Amyloid stimulates glial cells in vitro to produce growth factors that accumulate in senile plaques in Alzheimer's disease, Brain Res., 569, 141, 10.1016/0006-8993(92)90380-R · DOI
  3. Ard, 1996, Scavenging of Alzheimer's amyloid β-protein by microglia in culture, J. Neurosci. Res., 43, 190, 10.1002/(SICI)1097-4547(19960115)43:2<190::AID-JNR7>3.0.CO;2-B · DOI
  4. Ashwell, 1989, Development of microglia in the albino rabbit retina, J. Comp. Neurol., 287, 286, 10.1002/cne.902870303 · DOI
  5. Bell, 1994, Upregulation of the macrophage scavenger receptor in response to different forms of injury in the CNS, J. Neurocytol., 23, 605, 10.1007/BF01191555 · DOI
  6. Berbel, 1988, The development of the corpus callosum in cats: A light and electron-microscopic study, J. Comp. Neurol., 276, 132, 10.1002/cne.902760109 · DOI
  7. Bignami, 1991, Brain extracellular matrix and nerve regeneration, Adv. Exp. Med. Biol., 296, 197, 10.1007/978-1-4684-8047-4_19 · DOI
  8. Canning, 1993, β-amyloid of Alzheimer's disease induces reactive gliosis that inhibits axon outgrowth, Exp. Neurol., 124, 289, 10.1006/exnr.1993.1199 · DOI
  9. Chamak, 1991, Fibronectin and laminin regulate the in vitro differentiation of microglial cells, Neuroscience, 45, 513, 10.1016/0306-4522(91)90267-R · DOI
  10. Christie, 1996, Expression of the macrophage scavenger receptor, a multifunctional lipoprotein receptor, in microglia associated with senile plaques in Alzheimer's disease, Am. J. Pathol., 148, 399
  11. Cras, 1990, Neuronal and microglial involvement in β-amyloid protein deposition in Alzheimer's disease, Am. J. Pathol., 137, 241
  12. Davis, 1994, Cellular Forms and Functions of Brain Microglia, Brain Res. Bull., 34, 73, 10.1016/0361-9230(94)90189-9 · DOI
  13. Elkabes, 1996, Brain microglia/macrophages express neurotrophins that selectively regulate microglia proliferation and function, J. Neurosci., 16, 2508, 10.1523/JNEUROSCI.16-08-02508.1996 · DOI
  14. Ferrer, 1990, Naturally occurring cell death in the cerebral cortex of the rat and removal of dead cells by transitory phagocytes, Neuroscience, 39, 451, 10.1016/0306-4522(90)90281-8 · DOI
  15. Frackowiak, 1992, Ultrastructure of the microglia that phagocytose amyloid and the microglia that produce β-amyloid fibrils, Acta Neuropathol., 84, 225, 10.1007/BF00227813 · DOI · Paper page
  16. Frank, 1996, Cellular reaction at the lesion site after crushing of the rat optic nerve, Glia, 16, 227, 10.1002/(SICI)1098-1136(199603)16:3<227::AID-GLIA5>3.0.CO;2-Z · DOI
  17. Frautschy, 1992, Phagocytosis and deposition of vascular beta-amyloid in rat brains injected with Alzheimer beta amyloid, Am. J. Pathol., 140, 1389
  18. Gehrmann, 1995, Microglia: Intrinsic immunoeffector cell of the brain, Brain Res. Rev., 20, 269, 10.1016/0165-0173(94)00015-H · DOI
  19. Giordana, 1994, Reactive Cell Proliferation and microglia following injury to the rat brain, Neuropathol. Applied Neurobiol., 20, 163, 10.1111/j.1365-2990.1994.tb01175.x · DOI
  20. Giulian, 1986, Characterization of ameboid microglia isolated from developing mammalian brain, J. Neurosci., 6, 2163, 10.1523/JNEUROSCI.06-08-02163.1986 · DOI
  21. Giulian, 1989, The role of mononuclear phagocytes in wound healing after traumatic injury to adult mammalian brain, J. Neurosci., 9, 4416, 10.1523/JNEUROSCI.09-12-04416.1989 · DOI
  22. Giulian, 1995, Senile plaques stimulate microglia to release a neurotoxin found in Alzheimer's brain, Neurochem. Int., 27, 119, 10.1016/0197-0186(95)00067-I · DOI · Paper page
  23. Giulian, 1995, Cell surface morphology identifies microglia as a distinct class of mononuclear phagocyte, J. Neurosci., 15, 7712, 10.1523/JNEUROSCI.15-11-07712.1995 · DOI
  24. Hachimi, 1994, Do microglial cells phagocyte the β/A4-amyloid senile plaque core of Alzheimer disease, C. R. Acad. Sci. III, 317, 445
  25. Haga, 1989, Demonstration of microglia cells in and around senile (neuritic) plaques in the Alzheimer brain: An immunohistochemical study using a novel monoclonal antibody, Acta Neuropathol., 77, 569, 10.1007/BF00687883 · DOI
  26. Haga, 1993, Synthetic Alzheimer amyloid beta/A4 peptides enhance production of complement C3 component by cultured microglial cells, Brain Res, 601, 88, 10.1016/0006-8993(93)91698-R · DOI
  27. Hume, 1983, Immunohistochemical localization of a macrophage-specific antigen in developing mouse retina: phagocytosis of dying neurons and differentiation of microglial cells to form a regular array in the plexiform layers, J. Cell Biol., 97, 253, 10.1083/jcb.97.1.253 · DOI
  28. Hyman, 1993, The lack of accumulation of senile plaques or amyloid burden in Alzheimer's disease suggests a dynamic balance between amyloid deposition and resolution, J. Neuropathol. Exp. Neurol., 52, 594, 10.1097/00005072-199311000-00006 · DOI
  29. Innocenti, 1983, Transitory macrophages in the white matter of the developing visual cortex I. Light and electron microscopic characteristics and distribution, Brain Res., 313, 39, 10.1016/0165-3806(83)90200-6 · DOI
  30. Innocenti, 1983, Transitory macrophages in the white matter of the developing visual cortex. II. Development and relations with axonal pathways, Brain Res., 313, 55, 10.1016/0165-3806(83)90201-8 · DOI
  31. Killackey, 1984, Glia and the elimination of transient cortical projections, Trends Neurosci., 7, 225, 10.1016/S0166-2236(84)80209-X · DOI
  32. Kim, 1988, Production and characterization of monoclonal antibodies reactive to synthetic cerebrovascular amyloid peptide, Neurosci. Res. Commun., 2, 121
  33. Lawson, 1994, Quantification of the mononuclear phagocyte response to Wallerian degeneration of the optic nerve, J. Neurocytol., 23, 729, 10.1007/BF01268086 · DOI
  34. Linden, 1986, Mononuclear phagocytes in the retina of developing rats, Histochemistry, 85, 335, 10.1007/BF00493486 · DOI
  35. Liu, 1994, Macrophage colony-stimulating factor mediates astrocyte-induced microglial ramification in human fetal central nervous system culture, Am. J. Pathol., 145, 48
  36. Liu, 1997, Neuronal and glial apoptosis after traumatic spinal cord injury, J. Neurosci., 17, 5395, 10.1523/JNEUROSCI.17-14-05395.1997 · DOI · Paper page
  37. Mackenzie, 1995, Role of microglia in senile plaque formation, Neurobiol. Aging, 16, 797, 10.1016/0197-4580(95)00092-S · DOI · Paper page
  38. Mandybur, 1990, Astrocytes and the plaques of Alzheimer's disease, Neurology, 40, 635, 10.1212/WNL.40.4.635 · DOI
  39. McMillian, 1994, Brain injury in a dish: A model for reactive gliosis, Trends Neurosci., 17, 138, 10.1016/0166-2236(94)90086-8 · DOI
  40. Milligan, 1991, Differential immunocytochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain, J. Comp. Neurol., 314, 125, 10.1002/cne.903140112 · DOI
  41. Naruse, 1991, Mis-sense mutation Val-Ile in exon 17 of the amyloid precursor protein gene in Japanese familial Alzheimer's disease, Lancet, 337, 978, 10.1016/0140-6736(91)91612-X · DOI
  42. Nieto-Sampedro, 1994, Active microglia, sick astroglia and Alzheimer type dementias, Neuroreport, 5, 375, 10.1097/00001756-199401120-00001 · DOI
  43. Paresce, 1996, Microglial cells internalize aggregates of the Alzheimer's disease Amyloid β-Protein via a scavenger receptor, Neuron, 17, 553, 10.1016/S0896-6273(00)80187-7 · DOI · Paper page
  44. Perlmutter, 1992, MHC class II-positive microglia in human brain: association with Alzheimer lesions, J. Neurosci. Res., 33, 549, 10.1002/jnr.490330407 · DOI
  45. Perry, 1987, The macrophage response to central and peripheral nerve injury. A possible role for macrophages in regeneration, J. Exp. Med., 165, 1218, 10.1084/jem.165.4.1218 · DOI
  46. Perry, 1987, Macrophages and microglia in the nervous system, Trends Neuroci., 11, 273, 10.1016/0166-2236(88)90110-5 · DOI
  47. Pike, 1995, Early association of reactive astrocytes with senile plaques in Alzheimer's disease, Exp. Neurol., 132, 172, 10.1016/0014-4886(95)90022-5 · DOI · Paper page
  48. Rogers, 1992, Complement activation by β-amyloid in Alzheimer disease, Proc. Natl. Acad. Sci. USA, 89, 10016, 10.1073/pnas.89.21.10016 · DOI · Paper page
  49. Rosana, 1996, Microtubules can modulate pseudopod activity from a distance inside macrophages, Cell Motil. Cytoskeleton, 34, 230, 10.1002/(SICI)1097-0169(1996)34:3<230::AID-CM6>3.0.CO;2-D · DOI
  50. Selkoe, 1991, The molecular pathology of Alzheimer's disease, Neuron, 6, 487, 10.1016/0896-6273(91)90052-2 · DOI · Paper page
  51. Selkoe, 1986, Isolation of low-molecular-weight proteins from the amyloid plaque fibers in Alzheimer's disease, J. Neurochem., 46, 1820, 10.1111/j.1471-4159.1986.tb08501.x · DOI
  52. Shaffer, 1995, Amyloid β protein (Aβ) removal by neuroglial cells in culture, Neurobiol. Aging, 16, 737, 10.1016/0197-4580(95)00055-J · DOI
  53. Sievers, 1994, Blood monocytes and spleen macrophages differentiate into microglia-like cells on monolayers of Astrocytes: Morphology, Glia, 12, 245, 10.1002/glia.440120402 · DOI
  54. Stoll, 1989, Macrophage function during Wallerian degeneration of rat brain optic nerve: Clearance of degenerating myelin and Ia expression, J. Neurosci., 9, 2327, 10.1523/JNEUROSCI.09-07-02327.1989 · DOI
  55. Streit, 1990, An Improved Staining Method for Rat Microglial Cells Using the Lectin from Griffonia simplicifolia (GSA I-B4, J. Histochem. Cytochem., 38, 1683, 10.1177/38.11.2212623 · DOI
  56. Streit, 1988, Response of endogenous glial cells to motor neuron degeneration induced by toxin ricin, J. Comp. Neurol., 268, 248, 10.1002/cne.902680209 · DOI
  57. Suzuki, 1994, An increased percentage of long amyloid beta protein secreted by familial amyloid beta protein precursor (beta APP717) mutants, Science, 264, 1336, 10.1126/science.8191290 · DOI · Paper page
  58. Suzumura, 1991, Morphological transformation of microgliain vitro, Brain Res., 545, 301, 10.1016/0006-8993(91)91302-H · DOI
  59. Suzumura, 1993, Transforming Growth Factor-β Suppresses Activation and Proliferation of microgliain vitro, J. Immunol., 151, 2150, 10.4049/jimmunol.151.4.2150 · DOI
  60. Tanaka, 1996, Microglial ramification requires nondiffusible factors derived from astrocytes, Exp. Neurol., 137, 367, 10.1006/exnr.1996.0038 · DOI
  61. Thomas, 1992, Brain macrophages: evaluation of microglia and their functions, Brain Res. Rev., 17, 61, 10.1016/0165-0173(92)90007-9 · DOI
  62. Ward, 1991, Characterization of ramified microglia in tissue culture: Pinocytosis and motility, J. Neurosci. Res., 29, 13, 10.1002/jnr.490290103 · DOI
  63. Wisniewski, 1991, Phagocytosis of β/A4, Acta Neuropathol., 81, 588, 10.1007/BF00310142 · DOI
  64. Wisniewski, 1993, Migration of perivascular cells into the neuropil and their involvement in β-amyloid plaque formation, Acta Neuropathol., 85, 586, 10.1007/BF00334667 · DOI
  65. Wood, 1979, The failure of microglia in normal brain to exhibit mononuclear phagocyte markers, J. Neuropathol. Exp. Neurol., 38, 369, 10.1097/00005072-197907000-00002 · DOI

Cited by

Sorted by citations

Metadata

Authors
DeWitt, D. A., Perry, G., Cohen, M., Doller, C., & Silver, J.
Affiliations
Case Western Reserve University (US)
ORCID
Venue
Experimental Neurology
Publisher
Elsevier BV
Published
1998-02
Volume
149
Issue
2
Pages
329-340
ISSN
0014-4886 (ISSN-L)
DOI
10.1006/exnr.1997.6738
OpenAlex
W2164956116
Cited by
261
References
69
Citations by year
2026: 2 · 2025: 3 · 2024: 6 · 2023: 11 · 2022: 6 · 2021: 12 · 2020: 3 · 2019: 7 · 2018: 8 · 2017: 6 · 2016: 12 · 2015: 13 · 2014: 11 · 2013: 8 · 2012: 13
FWCI
6.82
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Experimental Neurology (publishedVersion)
  • PubMed (publishedVersion)
  • Scholars Crossing (Liberty University) (submittedVersion)
Funders
  • National Institutes of Health (5r01ag009287-08)
  • National Institute on Aging (AG-00105-11A1, T32AG000105, AG 09287)
  • National Institute of Neurological Disorders and Stroke (NS 25713)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
  • Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
Keywords
astrocytes, microglia, phagocytosis, amyloid plaques, Alzheimer's disease, plaque clearance, astrocyte-microglia interaction, conditioned medium, diffusible factors, latex bead phagocytosis, fibroblasts
MeSH terms
Alzheimer Disease, Animals, Animals, Newborn, Astrocytes, Brain, Cells, Cultured, Cerebral Cortex, Frontal Lobe, Humans, Phagocytosis, Temporal Lobe, Rats, Sprague-Dawley, Microglia, Coculture Techniques, Rats, Plaque, Amyloid

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review