The DOI matches its Crossref record; 23 of the 24 references it cites were found in a registry or an index.
Identification of a Mammalian Angiopoietin-Related Protein Expressed Specifically in Liver
Abstract
Based on searches of EST databases for signal sequences and amphipathic helices, we have identified and cloned an angiopoietin-like gene, ANGPTL3. Multiple tissue Northern blots show that ANGPTL3 is expressed principally in the liver. ANGPTL3 is expressed early during liver development, and expression is maintained in adult liver. Human ANGPTL3 is a 460-amino-acid polypeptide with the characteristic structure of angiopoietins: a signal peptide, an extended helical domain predicted to form dimeric or trimeric coiled-coils, a short linker peptide, and a globular fibrinogen homology domain (FHD). Murine ANGPTL3 is a 455-acid polypeptide encoded by seven exons on mouse chromosome 4, spanning about 11 kb of DNA. ANGPTL3 contains the four conserved cysteines implicated in the intramolecular disulfide bonds within the FHD, but it does not contain two other cysteines that are found within the FHD of angiopoietins 1, 2, and 4. ANGPTL3 also does not contain the characteristic calcium binding motif found in the other angiopoietins. By radiation hybrid mapping and the use of surrounding genes, human ANGPTL3 maps to the 1p31 region.
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In the citing paper
“They found that this protein, which they named ANGPTL3, was expressed almost exclusively in the liver in both mice and humans (103).”
From Regulation of lipoprotein metabolism by ANGPTL3, ANGPTL4, and ANGPTL8 (2021) · cites this paper as [103]
In the citing paper
“found a 460-amino acid protein with the conserved secondary structure of the ANGPTL family (a signal peptide followed by an N-terminal coil-coil domain with a linker leading to a C-terminal fibrinogen-like domain).”
From Regulation of lipoprotein metabolism by ANGPTL3, ANGPTL4, and ANGPTL8 (2021) · cites this paper as [103]
In the citing paper
“ANGPTL3 is expressed in and secreted from the liver and circulates in the bloodstream (15, 40, 72, 103).”
From Regulation of lipoprotein metabolism by ANGPTL3, ANGPTL4, and ANGPTL8 (2021) · cites this paper as [103]
In the citing paper
“ANGPTL3 is exclusively expressed and secreted by the liver and has emerged as an important regulator of plasma triglyceride levels due to its inhibition of LPL in oxidative tissues [34] (Figure 3).”
From Hepatokines and metabolism: Deciphering communication from the liver (2021) · cites this paper as [34]
Citations
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Isolation of Angiopoietin-1, a Ligand for the TIE2 Receptor, by Secretion-Trap Expression Cloning
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Angiopoietins 3 and 4: Diverging gene counterparts in mice and humans
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Cited by
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Köster, A., Chao, Y. B., Mosior, M., Ford, A., Gonzalez-DeWhitt, P. A., Hale, J. E., Li, D., Qiu, Y., Fraser, C. C., Yang, D. D., et al. (2005) · Endocrinology · doi:10.1210/en.2005-0476 · Cited by 427
Romeo, S., Yin, W., Kozlitina, J., Pennacchio, L. A., Boerwinkle, E., Hobbs, H. H., & Cohen, J. C. (2008) · Journal of Clinical Investigation · doi:10.1172/jci37118 · Cited by 58
Angptl3 regulates lipid metabolism in mice
Koishi, R., Ando, Y., Ono, M., Shimamura, M., Yasumo, H., Fujiwara, T., Horikoshi, H., & Furukawa, H. (2002) · Nature Genetics · doi:10.1038/ng814 · Cited by 379
Yoshida, K., Shimizugawa, T., Ono, M., & Furukawa, H. (2002) · Journal of Lipid Research · doi:10.1194/jlr.c200010-jlr200 · Cited by 317
Angiopoietin-like proteins stimulate ex vivo expansion of hematopoietic stem cells
Zhang, C. C., Kaba, M., Ge, G., Xie, K. T., Tong, W., Hug, C., & Lodish, H. F. (2006) · Nature Medicine · doi:10.1038/nm1342 · Cited by 372
Metadata
- Authors
- Conklin, D., Gilbertson, D., Taft, D. W., Maurer, M. F., Whitmore, T. E., Smith, D. L., Walker, K. M., Chen, L. H., Wattler, S., Nehls, M., & Lewis, K. B.
- Corresponding author
- Conklin, Darrell
- Venue
- Genomics
- Publisher
- Elsevier BV
- Published
- 1999-12
- Volume
- 62
- Issue
- 3
- Pages
- 477-482
- ISSN
- 0888-7543 (ISSN-L)
- OpenAlex
- W2033200911
- Cited by
- 169
- References
- 24
- Citations by year
- 2026: 8 · 2025: 11 · 2024: 11 · 2023: 13 · 2022: 12 · 2021: 14 · 2020: 11 · 2019: 8 · 2018: 5 · 2017: 5 · 2016: 2 · 2015: 8 · 2014: 3 · 2013: 7 · 2012: 3
- FWCI
- 1.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Genomics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- ANGPTL3, angiopoietin-like proteins, liver-specific gene expression, angiopoietins, coiled-coil domain, signal peptide, gene cloning, mouse chromosome 4, radiation hybrid mapping, liver development, cysteine disulfide bonds
- MeSH terms
- Angiopoietin-like Proteins, Angiopoietin-Like Protein 3, Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Chromosomes, Human, Pair 1, Glycosylation, Growth Substances, Humans, Liver, Membrane Glycoproteins, Molecular Sequence Data, Organ Specificity, Proteins, RNA, Messenger, Blotting, Northern, Sequence Homology, Amino Acid, Sequence Analysis, DNA, Physical Chromosome Mapping, Amino Acid Motifs, Intercellular Signaling Peptides and Proteins, Angiopoietins, Angiopoietin-1, Angiopoietin-2, Mice
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