Isolation of Angiopoietin-1, a Ligand for the TIE2 Receptor, by Secretion-Trap Expression Cloning
Open accessAbstract
TIE2 is a receptor-like tyrosine kinase expressed almost exclusively in endothelial cells and early hemopoietic cells and required for the normal development of vascular structures during embryogenesis. We report the identification of a secreted ligand for TIE2, termed Angiopoietin-1, using a novel expression cloning technique that involves intracellular trapping and detection of the ligand in COS cells. The structure of Angiopoietin-1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Although Angiopoietin-1 binds and induces the tyrosine phosphorylation of TIE2, it does not directly promote the growth of cultured endothelial cells. However, its expression in close proximity with developing blood vessels implicates Angiopoietin-1 in endothelial developmental processes.
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Ang-1 is an agonist of the Tie-2 receptor, which is constitutively expressed in several tissues, including AT.
“Ang-1 is an agonist of the Tie-2 receptor, which is constitutively expressed in several tissues, including AT (83)”
From Adipose tissue remodeling and obesity (2011) · cites this paper as [83]
Both Ang-1 and Ang-2 can bind to the common endothelial cell tyrosine receptor, Tie-2.
“Both Ang-1 and Ang-2 can bind to the common endothelial cell tyrosine receptor, Tie-2 (83,84)”
From Adipose tissue remodeling and obesity (2011) · cites this paper as [83]
Ang-2 is an antagonist for the Tie-2 receptor and is exclusively expressed in sites of vascular remodeling.
“Ang-2, on the other hand, is an antagonist for the Tie-2 receptor and is exclu-sively expressed in sites of vascular remodeling (83,84,88)”
From Adipose tissue remodeling and obesity (2011) · cites this paper as [83]
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- Authors
- Davis, S., Aldrich, T. H., Jones, P. F., Acheson, A., Compton, D. L., Jain, V., Ryan, T. E., Bruno, J., Radziejewski, C., Maisonpierre, P. C., & Yancopoulos, G. D.
- Affiliations
- Regeneron (United States) (US)
- Corresponding author
- Yancopoulos, George D
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1996-12
- Volume
- 87
- Issue
- 7
- Pages
- 1161-1169
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1970614272
- Cited by
- 1,989
- References
- 39
- Citations by year
- 2026: 15 · 2025: 32 · 2024: 26 · 2023: 29 · 2022: 33 · 2021: 35 · 2020: 49 · 2019: 50 · 2018: 39 · 2017: 50 · 2016: 57 · 2015: 54 · 2014: 66 · 2013: 67 · 2012: 71
- FWCI
- 21.46
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Regeneron Pharmaceuticals
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Keywords
- angiopoietin-1, Tie2 receptor, receptor tyrosine kinase, endothelial cells, vascular development, embryonic development, tyrosine phosphorylation, hematopoietic cells
- MeSH terms
- Amino Acid Sequence, Animals, Cattle, Cell Division, Cloning, Molecular, Endothelium, Vascular, Glycoproteins, Heart, Humans, Ligands, Membrane Glycoproteins, Molecular Sequence Data, Peptides, Protein-Tyrosine Kinases, Proteins, Signal Transduction, Sequence Alignment, Sequence Homology, Amino Acid, In Situ Hybridization, Protein Structure, Secondary, Gene Expression Regulation, Developmental, Neovascularization, Physiologic, Phosphotyrosine, Angiopoietin-1, Receptor, TIE-2, Mice, Rats
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