Cited by 1,989Angiogenesis and VEGF in CancerDevelopmental Biology and Gene RegulationAxon Guidance and Neuronal Signaling

Isolation of Angiopoietin-1, a Ligand for the TIE2 Receptor, by Secretion-Trap Expression Cloning

Open access
Davis, Samuel, Aldrich, Thomas H, Jones, Pamela F, Acheson, Ann, Compton, Debra L, Jain, Vivek, Ryan, Terence E, Bruno, Joanne +3 more·1996·Cell

Abstract

TIE2 is a receptor-like tyrosine kinase expressed almost exclusively in endothelial cells and early hemopoietic cells and required for the normal development of vascular structures during embryogenesis. We report the identification of a secreted ligand for TIE2, termed Angiopoietin-1, using a novel expression cloning technique that involves intracellular trapping and detection of the ligand in COS cells. The structure of Angiopoietin-1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Although Angiopoietin-1 binds and induces the tyrosine phosphorylation of TIE2, it does not directly promote the growth of cultured endothelial cells. However, its expression in close proximity with developing blood vessels implicates Angiopoietin-1 in endothelial developmental processes.

Integrity signals

Registry

Crossref · 10.1016/s0092-8674(00)81812-7

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Ang-1 is an agonist of the Tie-2 receptor, which is constitutively expressed in several tissues, including AT.

    “Ang-1 is an agonist of the Tie-2 receptor, which is constitutively expressed in several tissues, including AT (83)”

    From Adipose tissue remodeling and obesity (2011) · cites this paper as [83]

  • Both Ang-1 and Ang-2 can bind to the common endothelial cell tyrosine receptor, Tie-2.

    “Both Ang-1 and Ang-2 can bind to the common endothelial cell tyrosine receptor, Tie-2 (83,84)”

    From Adipose tissue remodeling and obesity (2011) · cites this paper as [83]

  • Ang-2 is an antagonist for the Tie-2 receptor and is exclusively expressed in sites of vascular remodeling.

    “Ang-2, on the other hand, is an antagonist for the Tie-2 receptor and is exclu-sively expressed in sites of vascular remodeling (83,84,88)”

    From Adipose tissue remodeling and obesity (2011) · cites this paper as [83]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Davis, S., Aldrich, T. H., Jones, P. F., Acheson, A., Compton, D. L., Jain, V., Ryan, T. E., Bruno, J., Radziejewski, C., Maisonpierre, P. C., & Yancopoulos, G. D.
Affiliations
Regeneron (United States) (US)
Corresponding author
Yancopoulos, George D
Venue
Cell
Publisher
Elsevier BV
Published
1996-12
Volume
87
Issue
7
Pages
1161-1169
ISSN
0092-8674 (ISSN-L)
OpenAlex
W1970614272
Cited by
1,989
References
39
Citations by year
2026: 15 · 2025: 32 · 2024: 26 · 2023: 29 · 2022: 33 · 2021: 35 · 2020: 49 · 2019: 50 · 2018: 39 · 2017: 50 · 2016: 57 · 2015: 54 · 2014: 66 · 2013: 67 · 2012: 71
FWCI
21.46
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
Regeneron Pharmaceuticals
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
Keywords
angiopoietin-1, Tie2 receptor, receptor tyrosine kinase, endothelial cells, vascular development, embryonic development, tyrosine phosphorylation, hematopoietic cells
MeSH terms
Amino Acid Sequence, Animals, Cattle, Cell Division, Cloning, Molecular, Endothelium, Vascular, Glycoproteins, Heart, Humans, Ligands, Membrane Glycoproteins, Molecular Sequence Data, Peptides, Protein-Tyrosine Kinases, Proteins, Signal Transduction, Sequence Alignment, Sequence Homology, Amino Acid, In Situ Hybridization, Protein Structure, Secondary, Gene Expression Regulation, Developmental, Neovascularization, Physiologic, Phosphotyrosine, Angiopoietin-1, Receptor, TIE-2, Mice, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review