Assessing Inflammatory Disease at Mucosal Surfaces in Murine Genetic Models
Abstract
Inflammatory diseases of the mucosal surfaces are rising worldwide and particularly in the Western world that is witnessing unprecedented increases in the number and incidence of both asthma and inflammatory bowel disease. The laboratory mouse allows the application of the full panoply of modern genetic, immunological and biochemical tools to increase our understanding of how inflammation arises and how it might be controlled at mucosal surfaces. Here we provide a detailed description of how to systematically assess inflammatory disease in the lung and intestines of the laboratory mouse. We provide histopathology examples from SHIP mutant mice that are the only known genetic mutant to suffer from pulmonary consolidation, asthma, and Crohn's disease. The intent of this chapter is to facilitate increased surveillance of mucosal inflammation in studies where the laboratory mouse is utilized so that we can better understand the cell types, genes, and microorganisms that contribute to mucosal inflammatory disease and thereby develop more effective therapies and preventive strategies.
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In vivo animal models possess limitations: species-specific differences between animals and humans cause differences in drug efficacy and toxicity tests in physiology and cell biology.
“Species-specific differences between animals and humans cause differences in drug efficacy and toxicity tests in physiology and cell biology31–37.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [31]
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Tumor-on-a-chip: from bioinspired design to biomedical application
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- Authors
- Engelman, R. W., & Kerr, W. G.
- Affiliations
- University of South Florida (US)
- SUNY Upstate Medical University (US)
- Corresponding author
- Kerr, William G.
- ORCID
- Kerr, William G.
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2012
- Volume
- 900
- Pages
- 433-441
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W2401869278
- Cited by
- 7
- References
- 45
- Citations by year
- 2024: 1 · 2021: 1 · 2019: 2 · 2016: 2 · 2015: 1
- FWCI
- 0.84
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl107127-04)
- National Heart, Lung, and Blood Institute (R01-HL107127, R01 HL72523, R01HL085580, R01HL072523)
- Topics
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- mucosal inflammation, asthma, inflammatory bowel disease, Crohn's disease, lung consolidation, lung histopathology, intestinal histopathology, genetic disease models
- MeSH terms
- Inositol Polyphosphate 5-Phosphatases, Animals, Eosinophils, Intestinal Mucosa, Mice, Transgenic, Models, Genetic, Molecular Biology, Phosphoric Monoester Hydrolases, Inflammatory Bowel Diseases, Mice
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