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Cited by 499

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A Rapid and General Assay for Monitoring Endogenous Gene Modification

Guschin, Dmitry Y., Waite, Adam J., Katibah, George E., Miller, Jeffrey C., Holmes, Michael C., Rebar, Edward J.·2010·Methods in Molecular Biology
DOI 10.1007/978-1-60761-753-2_15
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Abstract

The development of zinc finger nucleases for targeted gene modification can benefit from rapid functional assays that directly quantify activity at the endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand break repair via non-homologous end joining. The assay is based on the ability of the Surveyor nuclease to selectively cleave distorted duplex DNA formed via cross-annealing of mutated and wild-type sequence.

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Citations

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Show all 5 references

Research works printed but not linked: 19

  1. Cai, C.Q., Doyon, Y., Ainley, W.M., et al. (2009) Targeted transgene integration in plant cells using designed zinc finger nucleases. Plant Mol Biol. 69, 699–709. · DOI
  2. Doyon, Y., McCammon, J.M., Miller, J.C., et al. (2008) Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat Biotechnol. 26, 702–708. · DOI · Paper page
  3. Meng, X., Noyes, M.B., Zhu, L.J., Lawson, N.D., and Wolfe, S.A. (2008) Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol. 26, 695–701. · DOI · Paper page
  4. Moehle, E.A., Rock, J.M., Lee, Y.L., et al. (2007) Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases. Proc Natl Acad Sci USA. 104, 3055–3060. · DOI · Paper page
  5. Urnov, F.D., Miller, J.C., Lee, Y.L., et al. (2005) Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature. 435, 646–651. · DOI · Paper page
  6. Maeder, M.L., Thibodeau-Beganny, S., Osiak, A., et al. (2008) Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 31, 294–301. · DOI · Paper page
  7. Bibikova, M., Beumer, K., Trautman, J.K., and Carroll, D. (2003) Enhancing gene targeting with designed zinc finger nucleases. Science. 300, 764. · DOI · Paper page
  8. Beumer, K., Bhattacharyya, G., Bibikova, M., Trautman, J.K., and Carroll, D. (2006) Efficient gene targeting in Drosophila with zinc-finger nucleases. Genetics. 172, 2391–2403. · DOI · Paper page
  9. Wright, D.A., Townsend, J.A., Winfrey, R.J., Jr., et al. (2005) High-frequency homologous recombination in plants mediated by zinc-finger nucleases. Plant J. 44, 693–705. · DOI · Paper page
  10. Morton, J., Davis, M.W., Jorgensen, E.M., and Carroll, D. (2006) Induction and repair of zinc-finger nuclease-targeted double-strand breaks in Caenorhabditis elegans somatic cells. Proc Natl Acad Sci USA. 103, 16370–16375. · DOI · Paper page
  11. Carroll, D., Morton, J.J., Beumer, K.J., and Segal, D.J. (2006) Design, construction and in vitro testing of zinc finger nucleases. Nat Protoc. 1, 1329–1341. · DOI
  12. Mandell, J.G. and Barbas, C.F., 3rd. (2006) Zinc Finger Tools: custom DNA-binding domains for transcription factors and nucleases. Nucleic Acids Res. 34, W516–W523. · DOI · Paper page
  13. Sander, J.D., Zaback, P., Joung, J.K., Voytas, D.F., and Dobbs, D. (2007) Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool. Nucleic Acids Res. 35, W599–W605. · DOI · Paper page
  14. Jasin, M. (1996) Genetic manipulation of genomes with rare-cutting endonucleases. Trends Genet. 12, 224–228. · DOI · Paper page
  15. Valerie, K. and Povirk, L.F. (2003) Regulation and mechanisms of mammalian double-strand break repair. Oncogene. 22, 5792–5812. · DOI · Paper page
  16. Perez, E., Jouvenot, Y., Miller, J.C., et al (2006) Towards gene knock out therapy for AIDS/HIV: targeted disruption of CCR5 using engineered zinc finger protein nucleases (ZFNs). American Society of Gene Therapy, Baltimore.
  17. Qiu, P., Shandilya, H., D‘Alessio, J.M., O‘Connor, K., Durocher, J., and Gerard, G.F. (2004) Mutation detection using Surveyor nuclease. Biotechniques. 36, 702–707. · DOI · Paper page
  18. Santiago, Y., Chan, E., Liu, P.Q., et al. (2008) Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases. Proc Natl Acad Sci USA. 105, 5809–5814. · DOI · Paper page
  19. Rozen, S. and Skaletsky, H. (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 132, 365–386.

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Metadata

Authors
Guschin, D. Y., Waite, A. J., Katibah, G. E., Miller, J. C., Holmes, M. C., & Rebar, E. J.
Venue
Methods in Molecular Biology
Publisher
Humana Press
Published
2010
Volume
649
Pages
247-256
ISSN
  • 1064-3745 (ISSN-L)
  • 1940-6029
DOI
10.1007/978-1-60761-753-2_15
OpenAlex
W137643597
Cited by
499
References
5
FWCI
20.96
Type
book chapter
Language
en
Open access
closed

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CoChat. (2026). Citation check: A Rapid and General Assay for Monitoring Endogenous Gene Modification. https://app.cochat.ai/papers/10.1007/978-1-60761-753-2_15
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Guschin, D. Y., Waite, A. J., Katibah, G. E., Miller, J. C., Holmes, M. C., & Rebar, E. J. (2010). A Rapid and General Assay for Monitoring Endogenous Gene Modification. Methods in Molecular Biology. https://doi.org/10.1007/978-1-60761-753-2_15

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