Cited by 88Animal Virus Infections StudiesViral gastroenteritis research and epidemiologyAnimal Disease Management and Epidemiology

Studies on the relationship between coronaviruses from the intestinal and respiratory tracts of calves

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Reynolds, D. J., Debney, T. G., Hall, G. A., Thomas, L. H., Parsons, K. R.·1985·Archives of Virology

Abstract

An immunofluorescence test on smears of nasal epithelial cells was used to detect coronavirus infection in the respiratory tract of calves. Thirteen gnotobiotic calves were infected with coronavirus isolates derived from faeces or respiratory material: virus was detected in faeces and nasal swabs from all animals. In 115 calves from a field survey, there was a significant association between coronavirus excretion from both respiratory and enteric routes in calves with diarrhoea. In a further 12 calves, at necropsy, the predilection sites for coronavirus growth were the distal small intestine, large intestine and the epithelia of the nasal cavity and trachea. Antigen was not found in lung tissue by immunofluorescence or immunoperoxidase staining. Infection with enteric coronavirus induced immunity to reinfection and to heterologous challenge with two coronavirus isolates derived from the respiratory tract. Nine coronaviruses were cultivated, cloned and antisera to three were prepared in pigs. There was complete virus neutralisation in tests with homologous sera and significant cross reactions with the eight other isolates which were of intestinal and respiratory origin. Thus, these bovine coronavirus isolates belonged to the same serotype despite the source of virus.

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Crossref · 10.1007/bf01317007

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Authors
Reynolds, D. J., Debney, T. G., Hall, G. A., Thomas, L. H., & Parsons, K. R.
Affiliations
University of Reading (GB)
Venue
Archives of Virology
Published
1985-03
Volume
85
Issue
1-2
Pages
71-83
ISSN
  • 0003-9012 (ISSN-L)
  • 0304-8608
  • 1432-8798
OpenAlex
W1976074519
Cited by
88
References
23
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 3 · 2012: 4
FWCI
3.20
Type
journal article
Language
en
Open access
Locations
  • Archives of Virology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Animal Disease Management and Epidemiology
Keywords
bovine coronavirus, calves, enteric coronavirus, viral excretion, diarrhea, viral tissue distribution, nasal epithelium, intestinal immunity, cross-neutralization, serotypes, virus isolation, gnotobiotic calves
MeSH terms
Animals, Antigens, Viral, Cattle, Cattle Diseases, Coronaviridae, Coronaviridae Infections, Feces, Fluorescent Antibody Technique, Immunity, Intestines, Neutralization Tests, Respiratory System, Virus Replication

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