Monitoring the growth and stress responses of yeast cells by two-dimensional fluorescence spectroscopy: First results
Abstract
S. cerevisiae growth and responses to different treatments were monitored by two-dimensional fluorescence spectroscopy, which simultaneously detects the fluorescence of a number of cells' own fluorophores. Growth curves of cultures of free cells were measured by means of tryptophan fluorescence in nonfluorescent culture medium and a flow-through system at a suitable excitation/emission beam geometry. Fast responses of the cells to anaerobic-aerobic transition or addition of glucose, methanol or cyanide, which could not be measured in this system because of the time delay inherent in transporting the cells from the culture flask to the cuvette, were monitored with cells immobilized in alginate. The major fluorescence changes caused by these treatments belonged to NAD(P)H which is a good indicator of the redox state of the cells.
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Fluorescence spectroscopy was used to monitor biomass and substrate or product concentrations in E. coli and S. cerevisiae fermentations.
“Fluorescence spectroscopy was also used to monitor biomass and substrate or product concentrations in E. coli and S. cerevisiae fermentations (Marose et al., 1999; Podrazký et al., 2003).”
From Perspectives of fluorescence spectroscopy for online monitoring in microalgae industry (2022) · cites this paper as Podrazký et al., 2003
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- Authors
- Podrazký, O., Kuncová, G., Krasowska, A., & Sigler, K.
- Affiliations
- Czech Academy of Sciences (CZ)
- University of Wrocław (PL)
- Venue
- Folia Microbiologica
- Published
- 2003-03
- Volume
- 48
- Issue
- 2
- Pages
- 189-192
- ISSN
- 0015-5632 (ISSN-L)
- 1874-9356
- OpenAlex
- W1973224200
- Cited by
- 22
- References
- 9
- Citations by year
- 2026: 2 · 2023: 2 · 2022: 3 · 2021: 1 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 2 · 2013: 1
- FWCI
- 1.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Folia Microbiologica (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Keywords
- Saccharomyces cerevisiae, two-dimensional fluorescence spectroscopy, cell growth monitoring, stress response, tryptophan fluorescence, NAD(P)H, redox state, glucose supplementation, methanol, cyanide, immobilized cells, alginate, flow-through system
- MeSH terms
- Methanol, Alginates, Culture Media, Hexuronic Acids, NADP, Potassium Cyanide, Saccharomyces cerevisiae, Spectrometry, Fluorescence, Heat-Shock Response, Cells, Immobilized, Glucuronic Acid
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