Two-Dimensional Fluorescence Spectroscopy: A New Tool for On-Line Bioprocess Monitoring
Abstract
Two-dimensional fluorescence spectroscopy is presented as a new method for bioprocess monitoring. It covers a wide range of excitation and emission wavelengths and is a further development of the fluorescence measurements performed so far, which concentrated mainly on NAD(P)H culture fluorescence. Biogenic fluorophores such as proteins, coenzymes, and vitamins can simultaneously be detected qualitatively and quantitatively inside and outside the cells. This optical method is noninvasive, suitable for in vivo measurements. One whole spectrum (excitation, 250-550 nm; emission, 260-600 nm) with the described parameters is performed within 1 min, which allows an almost continuous monitoring of the bioprocess. The technique is ideal for on-line, in situ measurements via fiber optical systems. Results are presented for cultivations of Claviceps purpurea, Escherichia coli, Saccharomyces cerevisiae, and Sphingomonas yanoikuyae. Cell growth and the metabolism of the cells (changes from aerobic to anaerobic conditions and uncoupling of the oxidative phosphorylation) could be detected.
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- Authors
- Marose, S., Lindemann, C., & Scheper, T.
- Affiliations
- Leibniz University Hannover (DE)
- Corresponding author
- Scheper, T.
- Venue
- Biotechnology Progress
- Publisher
- Wiley
- Published
- 1998-02-06
- Volume
- 14
- Issue
- 1
- Pages
- 63-74
- ISSN
- 1520-6033 (ISSN-L)
- 8756-7938
- OpenAlex
- W2037435747
- Cited by
- 190
- References
- 0
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 3 · 2023: 9 · 2022: 9 · 2021: 5 · 2020: 1 · 2019: 6 · 2018: 6 · 2017: 8 · 2016: 9 · 2015: 4 · 2014: 6 · 2013: 5 · 2012: 11
- FWCI
- 5.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology Progress (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Environmental Science › Waste Management and Disposal › Water Quality Monitoring and Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- two-dimensional fluorescence spectroscopy, bioprocess monitoring, real-time process monitoring, NAD(P)H fluorescence, aerobic-anaerobic transition, mitochondrial uncoupling, Claviceps purpurea, Escherichia coli, Saccharomyces cerevisiae
- MeSH terms
- Biotechnology, Cells, Cultured, Claviceps, Culture Media, Escherichia coli, Flavobacterium, Fluorescent Dyes, Online Systems, Saccharomyces cerevisiae, Spectrometry, Fluorescence
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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