The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Effects of weight loss and exercise on insulin resistance, and intramyocellular triacylglycerol, diacylglycerol and ceramide
Open accessAbstract
Aims/hypothesisIntramyocellular lipids, including diacylglycerol (DAG) and ceramides, have been linked to insulin resistance. This randomised repeated-measures study examined the effects of diet-induced weight loss (DIWL) and aerobic exercise (EX) on insulin sensitivity and intramyocellular triacylglycerol (IMTG), DAG and ceramide.MethodsSixteen overweight to obese adults (BMI 30.6 ± 0.8; 67.2 ± 4.0 years of age) with either impaired fasting glucose, or impaired glucose tolerance completed one of two lifestyle interventions: DIWL (n = 8) or EX (n = 8). Insulin sensitivity was determined using hyperinsulinaemic–euglycaemic clamps. Intramyocellular lipids were measured in muscle biopsies using histochemistry and tandem mass spectrometry.ResultsInsulin sensitivity was improved with DIWL (20.6 ± 4.7%) and EX (19.2 ± 12.9%). Body weight and body fat were decreased by both interventions, with greater decreases in DIWL compared with EX. Muscle glycogen, IMTG content and oxidative capacity were all significantly (p < 0.05) decreased with DIWL and increased with EX. There were decreases in DAG with DIWL (−12.4 ± 14.6%) and EX (−40.9 ± 12.0%). Ceramide decreased with EX (−33.7 ± 11.2%), but not with DIWL. Dihydroceramide was decreased with both interventions. Sphingosine was decreased only with EX. Changes in total DAG, total ceramides and other sphingolipids did not correlate with changes in glucose disposal. Stearoyl-coenzyme A desaturase 1 (SCD1) content was decreased with DIWL (−19.5 ± 8.5%, p < 0.05), but increased with EX (19.6 ± 7.4%, p < 0.05). Diacylglycerol acyltransferase 1 (DGAT1) was unchanged with the interventions.Conclusions/interpretationDiet-induced weight loss and exercise training both improved insulin resistance and decreased DAG, while only exercise decreased ceramides, despite the interventions having different effects on IMTG. These alterations may be mediated through differential changes in skeletal muscle capacity for oxidation and triacylglycerol synthesis.Trial registration:ClinicalTrials.gov NCT00766298Funding:ADA Clinical Research Award (B. H. Goodpaster), NIH R01 AG20128 (B. H. Goodpaster), Obesity and Nutrition Research (1P30DK46204) and Clinical and Translational Research Centers (UL1 RR024153)
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Yu, C., Chen, Y., Cline, G. W., Zhang, D., Zong, H., Wang, Y., Bergeron, R., Kim, J. K., Cushman, S. W., Cooney, G. J., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m200958200 · Cited by 1,561
Goodpaster, B. H. (2001) · Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.86.12.5755 · Cited by 1,194
Self-citationBouchard, C., An, P., Rice, T., Skinner, J. S., Wilmore, J. H., Gagnon, J., Pérusse, L., Leon, A. S., & Rao, D. C. (1999) · Journal of Applied Physiology · doi:10.1152/jappl.1999.87.3.1003 · Cited by 875
Bioactive sphingolipids: metabolism and function
Bartke, N., & Hannun, Y. A. (2009) · Journal of Lipid Research · doi:10.1194/jlr.r800080-jlr200 · Cited by 683
Chavez, J. A., Knotts, T. A., Wang, L.-P., Li, G., Dobrowsky, R. T., Florant, G. L., & Summers, S. A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212307200 · Cited by 617
Effects of weight loss on regional fat distribution and insulin sensitivity in obesity.
Goodpaster, B. H., Kelley, D. E., Wing, R. R., Meier, A., & Thaete, F. L. (1999) · Diabetes · doi:10.2337/diabetes.48.4.839 · Cited by 608
Self-citationIntramuscular lipid content is increased in obesity and decreased by weight loss
Goodpaster, B. H., Theriault, R., Watkins, S. C., & Kelley, D. E. (2000) · Metabolism · doi:10.1016/s0026-0495(00)80010-4 · Cited by 526 · cited 3×
“Thus, although weight loss and exercise can both improve insulin sensitivity [7, 16] they may have distinctly different effects on IMCL [6, 9].”
BackgroundMethodSelf-citationTarnopolsky, M. A., Rennie, C. D., Robertshaw, H. A., Fedak-Tarnopolsky, S. N., Devries, M. C., & Hamadeh, M. J. (2007) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00472.2006 · Cited by 457
Schenk, S., & Horowitz, J. F. (2007) · Journal of Clinical Investigation · doi:10.1172/jci30566 · Cited by 407
Phillips, D. I. W., Caddy, S., Ilic, V., Fielding, B. A., Frayn, K. N., Borthwick, A. C., & Taylor, R. (1996) · Metabolism · doi:10.1016/s0026-0495(96)90260-7 · Cited by 390 · cited 1×
“Early evidence from cell culture [3], animal models [4] and human studies [5] strongly suggested that accumulation of intramyocellular triacylglycerol (IMTG) was responsible for insulin resistance of obesity and type 2 diabetes.”
BackgroundDubé, J. J., Amati, F., Stefanovic-Racic, M., Toledo, F. G. S., Sauers, S. E., & Goodpaster, B. H. (2008) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00769.2007 · Cited by 378
Self-citationObesity-related derangements in metabolic regulation
Muoio, D. M., & Newgard, C. B. (2006) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.75.103004.142512 · Cited by 345
Bruce, C. R., Thrush, A. B., Mertz, V. A., Bezaire, V., Chabowski, A., Heigenhauser, G. J. F., & Dyck, D. J. (2006) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00587.2005 · Cited by 328
Exercise training increases intramyocellular lipid and oxidative capacity in older adults
Pruchnic, R., Katsiaras, A., He, J., Kelley, D. E., Winters, C., & Goodpaster, B. H. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00459.2003 · Cited by 177
Self-citationHoy, A. J., Brandon, A. E., Turner, N., Watt, M. J., Bruce, C. R., Cooney, G. J., & Kraegen, E. W. (2009) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90945.2008 · Cited by 92
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Physical activity/exercise and diabetes: A position statement of the American Diabetes Association
Colberg, S. R., Sigal, R. J., Yardley, J. E., Riddell, M. C., Dunstan, D. W., Dempsey, P. C., Horton, E. S., Castorino, K., & Tate, D. F. (2016) · Diabetes Care · doi:10.2337/dc16-1728 · Cited by 2,991
Update on the effects of physical activity on insulin sensitivity in humans
Bird, S. R., & Hawley, J. A. (2017) · BMJ Open Sport & Exercise Medicine · doi:10.1136/bmjsem-2016-000143 · Cited by 646
Madeo, F., Carmona-Gutierrez, D., Hofer, S. J., & Kroemer, G. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.01.018 · Cited by 630
Skeletal Muscle Triglycerides, Diacylglycerols, and Ceramides in Insulin Resistance
Amati, F., Dubé, J. J., Alvarez-Carnero, E., Edreira, M. M., Chomentowski, P., Coen, P. M., Switzer, G. E., Bickel, P. E., Stefanovic-Racic, M., Toledo, F. G. S., et al. (2011) · Diabetes · doi:10.2337/db10-1221 · Cited by 395
Self-citationEctopic fat and insulin resistance: pathophysiology and effect of diet and lifestyle interventions.
Snel, M., Jonker, J. T., Schoones, J., Lamb, H., de Roos, A., Pijl, H., Smit, J. W. A., Meinders, A. E., & Jazet, I. M. (2012) · International Journal of Endocrinology · doi:10.1155/2012/983814 · Cited by 368
Metadata
- Authors
- Dubé, J. J., Amati, F., Toledo, F. G. S., Stefanovic-Racic, M., Rossi, A., Coen, P., & Goodpaster, B. H.
- Venue
- Diabetologia
- Published
- 2011-02-17
- Volume
- 54
- Issue
- 5
- Pages
- 1147-1156
- ISSN
- 0012-186X (ISSN-L)
- 1432-0428
- OpenAlex
- W2024872626
- Cited by
- 271
- References
- 15
- FWCI
- 12.71
- Type
- journal article
- Language
- en
- Open access
- bronze
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