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Cited by 85Bacterial biofilms and quorum sensingBacterial Genetics and BiotechnologyMicrobial bioremediation and biosurfactants

Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth with sodium dodecyl sulfate

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Klebensberger, Janosch, Rui, Oliver, Fritz, Eva, Schink, Bernhard, Philipp, Bodo·2006·Archives of Microbiology
DOI 10.1007/s00203-006-0111-y
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Abstract

Pseudomonas aeruginosa strain PAO1 grew with the detergent sodium dodecyl sulfate (SDS). The growth started with the formation of macroscopic cell aggregates which consisted of respiring cells embedded in an extracellular matrix composed of acidic polysaccharides and DNA. Damaged and uncultivable cells accumulated in these aggregates compared to those cells that remained suspended. We investigated the response of suspended cells to SDS under different conditions. At high energy supply, the cells responded with a decrease in optical density and in viable counts, release of protein and DNA, and formation of macroscopic aggregates. This response was not observed if the energy supply was reduced by inhibiting respiration with KCN, or if cells not induced for SDS degradation were exposed to SDS. Exposure to SDS caused cell lysis without aggregation if cells were completely deprived of energy, either by applying anoxic conditions, by addition of CCCP, or by addition of KCN to a mutant defective in cyanide-insensitive respiration. Aggregated cells showed a more than 100-fold higher survival rate after exposure to SDS plus CCCP than suspended cells. Our results demonstrate that cell aggregation is an energy-dependent response of P. aeruginosa to detergent stress which might serve as a survival strategy during growth with SDS.

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  • The active formation of cell aggregates is a stress response in P. aeruginosa and serves as a survival mechanism when exposed to certain detergents such as sodium dodecyl sulfate (SDS).

    “The active formation of cell aggregates is also a stress response in P. aeruginosa and serves as a survival mechanism when exposed to certain detergents such as sodium dodecyl sulfate (SDS) [106],[107].”

    From Bacterial autoaggregation (2018) · cites this paper as [106]

    The Discussion describes energy-dependent aggregation as an active response to SDS and reports that cells in aggregates were better protected than suspended cells during SDS-plus-CCCP exposure. It says aggregation *could be* a survival strategy during growth with SDS; the citing sentence presents that proposed function as established and extends it to detergents beyond the SDS studied here. (LLM, conf=0.86)

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Related

Authors
Klebensberger, J., Rui, O., Fritz, E., Schink, B., & Philipp, B.
Affiliations
University of Konstanz (DE)
Corresponding author
Philipp, Bodo
ORCID
Venue
Archives of Microbiology
Publisher
Springer Science and Business Media LLC
Published
2006-04-26
Volume
185
Issue
6
Pages
417-427
ISSN
  • 0003-9276 (ISSN-L)
  • 0302-8933
  • 1432-072X
DOI
10.1007/s00203-006-0111-y
OpenAlex
W2157669839
Cited by
85
References
49
Citations by year
2026: 3 · 2025: 2 · 2024: 2 · 2023: 6 · 2022: 6 · 2021: 9 · 2020: 3 · 2019: 3 · 2018: 6 · 2017: 4 · 2016: 3 · 2015: 7 · 2014: 3 · 2013: 3 · 2012: 7
FWCI
1.63
Type
journal article
Language
en
Open access
Licence
Locations
  • Archives of Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • KOPS (University of Konstanz) (submittedVersion, cc-by-nc-nd)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
Keywords
Pseudomonas aeruginosa PAO1, cell aggregation, sodium dodecyl sulfate, extracellular matrix, cell lysis, cyanide-resistant respiration, respiration inhibition, CCCP, potassium cyanide, anoxia, survival strategies
MeSH terms
Carbonyl Cyanide m-Chlorophenyl Hydrazone, Cell Division, Energy Metabolism, Pseudomonas aeruginosa, Sodium Dodecyl Sulfate, Time Factors, Microbial Viability

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