SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa
Open accessAbstract
Cell aggregation is a stress response and serves as a survival strategy for Pseudomonas aeruginosa strain PAO1 during growth with the toxic detergent Na-dodecylsulfate (SDS). This process involves the psl operon and is linked to c-di-GMP signalling. The induction of cell aggregation in response to SDS was studied. Transposon and site-directed mutagenesis revealed that the cupA-operon and the co-transcribed genes siaA (PA0172) and siaD (PA0169) were essential for SDS-induced aggregation. While siaA encodes a putative membrane protein with a HAMP and a PP2C-like phosphatase domain, siaD encodes a putative diguanylate cyclase involved in the biosynthesis of c-di-GMP. Complementation studies uncovered that the loss of SDS-induced aggregation in the formerly isolated spontaneous mutant strain N was caused by a non-functional siaA allele. DNA-microarray analysis of SDS-grown cells revealed consistent activation of eight genes, including cupA1, with known or presumptive important functions in cell aggregation in the parent strain compared with non-aggregating siaA and siaD mutants. A siaAD-dependent increase of cupA1 mRNA levels in SDS-grown cells was also shown by Northern blots. These results clearly demonstrate that SiaAD are essential for inducing cell aggregation as a specific response to SDS and suggest that they are responsible for perceiving and transducing SDS-related stress.
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SiaD is predicted to encode a putative cytoplasmic di-guanylate cyclase involved in the biosynthesis of cyclic di-guanosine monophosphate (c-di-GMP).
“SiaD is predicted to encode a putative cytoplasmic di-guanylate cyclase involved in the biosynthesis of cyclic di-guanosine monophosphate (c-di-GMP), thus suggesting that SDS-induced aggregation is regulated through a c-di-GMP-dependent signal transduction pathway [108].”
From Bacterial autoaggregation (2018) · cites this paper as [108]
SDS-induced aggregation in P. aeruginosa is regulated through a c-di-GMP-dependent signal transduction pathway.
“SiaD is predicted to encode a putative cytoplasmic di-guanylate cyclase involved in the biosynthesis of cyclic di-guanosine monophosphate (c-di-GMP), thus suggesting that SDS-induced aggregation is regulated through a c-di-GMP-dependent signal transduction pathway [108].”
From Bacterial autoaggregation (2018) · cites this paper as [108]
The genes siaA and siaD are essential for induction of autoaggregation as a specific response to SDS in P. aeruginosa.
“The genes siaA (SDS-induced-aggregation A) and siaD are essential for induction of autoaggregation as a specific response to SDS and have been suggested to be responsible for perceiving and transducing SDS-related stress [108].”
From Bacterial autoaggregation (2018) · cites this paper as [108]
SiaA encodes a putative membrane protein harboring a HAMP domain and a PP2C-like phosphatase domain.
“SiaA encodes a putative membrane protein harboring a HAMP (histidine kinases, adenylyl cyclases, methyl-accepting chemotaxis proteins and phosphatases) domain and a PP2C (Protein phosphatase 2C)-like phosphatase domain.”
From Bacterial autoaggregation (2018) · cites this paper as [108]
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Trunk, T., Bacterial Cell Surface Group, S. F. G. A. E. B., S. Khalil, H., & C. Leo, J. (2018) · AIMS Microbiology · doi:10.3934/microbiol.2018.1.140 · Cited by 444 · see citing works
“of autoaggregation as a specific response to SDS and have been suggested to be responsible for perceiving and transducing SDS-related stress [108].”
Background
- Authors
- Klebensberger, J., Birkenmaier, A., Geffers, R., Kjelleberg, S., & Philipp, B.
- Affiliations
- University of Konstanz (DE)
- Helmholtz Centre for Infection Research (DE)
- UNSW Sydney (AU)
- Corresponding author
- Philipp, Bodo
- Venue
- Environmental Microbiology
- Publisher
- Wiley
- Published
- 2009-12
- Volume
- 11
- Issue
- 12
- Pages
- 3073-3086
- ISSN
- 1462-2912 (ISSN-L)
- 1462-2920
- OpenAlex
- W2132171052
- Cited by
- 97
- References
- 60
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 5 · 2023: 3 · 2022: 6 · 2021: 9 · 2020: 13 · 2019: 6 · 2018: 6 · 2017: 4 · 2016: 10 · 2015: 7 · 2014: 7 · 2013: 7 · 2012: 3
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- green
- nbn-resolving.de
- Locations
- Environmental Microbiology (publishedVersion)
- PubMed (publishedVersion)
- KOPS (University of Konstanz) (submittedVersion)
- HZI (Helmholtz Centre for Infection Research) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SFB 454)
- Universität Konstanz
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Keywords
- Pseudomonas aeruginosa, cell aggregation, self-aggregation, sodium dodecyl sulfate, c-di-GMP signalling, diguanylate cyclase, HAMP domain, stress response, gene expression
- MeSH terms
- Detergents, Genes, Bacterial, Mutation, Pseudomonas aeruginosa, Sodium Dodecyl Sulfate, Stress, Physiological, Gene Expression Regulation, Bacterial
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