Cited by 278Algal biology and biofuel productionFatty Acid Research and HealthMicrobial Metabolic Engineering and Bioproduction

The DOI matches its Crossref record; 58 of the 60 references it cites were found in a registry or an index.

Biotechnological production and applications of the ω-3 polyunsaturated fatty acid docosahexaenoic acid

Open access

Sijtsma, L., de Swaaf, M. E.·2004·Applied Microbiology and Biotechnology

DOI 10.1007/s00253-003-1525-y

Open this paper in CoChat — free account

Abstract

Docosahexaenoic acid (DHA) is a polyunsaturated fatty acid composed of 22 carbon atoms and six double bonds. Because the first double bond, as counted from the methyl terminus, is at position three, DHA belongs to the so-called omega-3 group. In recent years, DHA has attracted much attention because of its beneficial effect on human health. At present, fish oil is the major source of DHA, but alternatively it may be produced by use of microorganisms. Marine microorganisms may contain large quantities of DHA and are considered a potential source of this important fatty acid. Some of these organisms can be grown heterotrophically on organic substrates without light. These processes can be well controlled and DHA with constant quality can be produced all year round. This paper reviews recent advances in the biotechnological production of DHA by marine microorganisms.

Integrity signals

Registry

Crossref · 10.1007/s00253-003-1525-y

Cites

58 of 58 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

HEALTH BENEFITS OF DOCOSAHEXAENOIC ACID (DHA)
Horrocks, L. A., & Yeo, Y. K. (1999) · Pharmacological Research · doi:10.1006/phrs.1999.0495 · Cited by 1,055
Photobioreactors: Production systems for phototrophic microorganisms
O., P. (2001) · Applied Microbiology and Biotechnology · doi:10.1007/s002530100702 · Cited by 987
The lipid handbook
Gunstone, F. D., Harwood, J. L., & Padley, F. B. (1994) · Cited by 972
Epidemiological studies in the Upernavik district, Greenland. Incidence of some chronic diseases 1950-1974.
Kromann, N., & Green, A. (1980) · PubMed · Cited by 854
Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes.
Metz, J. G., Roessler, P., Facciotti, D., Levering, C., Dittrich, F., Lassner, M., Valentine, R., Lardizabal, K., Domergue, F., Yamada, A., et al. (2001) · Science · doi:10.1126/science.1059593 · Cited by 760
Polyunsaturated fatty acids, part 1: Occurrence, biological activities and applications
Gill, I., & Valivety, R. H. (1997) · Trends in biotechnology · doi:10.1016/s0167-7799(97)01076-7 · Cited by 431
Fatty acids from 28 marine microalgae
Viso, A.-C., & Marty, J.-C. (1993) · Phytochemistry · doi:10.1016/s0031-9422(00)90839-2 · Cited by 400
Fatty Acid and Lipid Chemistry
Gunstone, F. D. (1996) · Springer eBooks · doi:10.1007/978-1-4615-4131-8 · Cited by 393
High cell density culture of microalgae in heterotrophic growth
Chen, F. (1996) · Trends in biotechnology · doi:10.1016/0167-7799(96)10060-3 · Cited by 354
Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21
Yokochi, T., Honda, D., Higashihara, T., & Nakahara, T. (1998) · Applied Microbiology and Biotechnology · doi:10.1007/s002530051139 · Cited by 352

Show all 60 references

Sorted by citations

Items 101–200 of 278

Recombinant production of omega-3 fatty acids in Escherichia coli using a gene cluster isolated from Shewanella baltica MAC1
Amiri-Jami, M., & Griffiths, M. W. (2010) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2010.04817.x · Cited by 44
Engineering of EPA/DHA omega-3 fatty acid production by Lactococcus lactis subsp. cremoris MG1363
Amiri-Jami, M., LaPointe, G., & Griffiths, M. W. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-5381-0 · Cited by 43
Aquaculture: An overview of chemical ecology of seaweeds (food species) in natural products
Gnanavel, V., Roopan, S. M., & Rajeshkumar, S. (2019) · Aquaculture · doi:10.1016/j.aquaculture.2019.04.004 · Cited by 43
Nutrition, Health, and Disease: Role of Selected Marine and Vegetal Nutraceuticals
Corzo, L., Fernández‐Novoa, L., Carrera, I., Martínez, O., Rodríguez, S., Alejo, R., & Cacabelos, R. (2020) · Nutrients · doi:10.3390/nu12030747 · Cited by 44
Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes
Fidio, N. D., Minonne, F., Antonetti, C., & Galletti, A. M. R. (2021) · Catalysts · doi:10.3390/catal11111291 · Cited by 42
Molecular identification of marine yeast and its spectroscopic analysis establishes unsaturated fatty acid accumulation
Gupta, A., Vongsvivut, J. P., Barrow, C. J., & Puri, M. (2012) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2012.05.013 · Cited by 42
Optimization of the production of docosahexaenoic fatty acid by the heterotrophic microalga Crypthecodinium cohnii utilizing a dark fermentation effluent
Chalima, A., Taxeidis, G., & Topakas, E. (2020) · Renewable Energy · doi:10.1016/j.renene.2020.01.041 · Cited by 42
Recent advances in marine biotechnology
Chakraborty, K. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-91240-2.00002-6 · Cited by 42
Botryococcus braunii and Nannochloropsis oculata extracts inhibit cholinesterases and protect human dopaminergic SH-SY5Y cells from H2O2-induced cytotoxicity
Custódio, L. M. B., Soares, F., Pereira, H., Rodrigues, M. J., Barreira, L. A., Rauter, A. P., Alberício, F., & Varela, J. C. S. (2014) · Journal of Applied Phycology · doi:10.1007/s10811-014-0369-4 · Cited by 43
Effect of culture conditions on growth, lipid content, and fatty acid composition of Aurantiochytrium mangrovei strain BL10
Chaung, K.-C., Chu, C.-Y., Su, Y.-M., & Chen, Y.-M. (2012) · AMB Express · doi:10.1186/2191-0855-2-42 · Cited by 41
Selecting Australian marine macroalgae based on the fatty acid composition and anti-inflammatory activity
McCauley, J. I., Meyer, B. J., Winberg, P. C., Ranson, M., & Skropeta, D. (2014) · Journal of Applied Phycology · doi:10.1007/s10811-014-0465-5 · Cited by 43
Life cycle assessment of industrial production of microalgal oil from heterotrophic fermentation
Lu, Y., Mu, D., Xue, Z., Xu, P., Li, Y., Xiang, W., Burnett, J., Bryant, K., & Zhou, W. (2021) · Algal Research · doi:10.1016/j.algal.2021.102404 · Cited by 39
Efficient production of triacylglycerols rich in docosahexaenoic acid (DHA) by osmo-heterotrophic marine protists
Liu, Y., Tang, J., Li, J., Daroch, M., & Cheng, J. J. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6032-9 · Cited by 39
An n-3 PUFA-rich microalgal oil diet protects to a similar extent as a fish oil-rich diet against AOM-induced colonic aberrant crypt foci in F344 rats
van Beelen, V. A., Spenkelink, B., Mooibroek, H., Sijtsma, L., Bosch, D., Rietjens, I. M. C. M., & Alink, G. M. (2008) · Food and Chemical Toxicology · doi:10.1016/j.fct.2008.11.014 · Cited by 39
Self-citation
Dietary Supplements with Antiplatelet Activity: A Solution for Everyone?
Olas, B. (2018) · Advances in Nutrition · doi:10.1093/advances/nmx014 · Cited by 38
Assessment of Fatty Acids Profile and Omega-3 Polyunsaturated Fatty Acid Production by the Oleaginous Marine Thraustochytrid Aurantiochytrium sp. T66 Cultivated on Volatile Fatty Acids
Patel, A., Rova, U., Christakopoulos, P., & Μάτσακας, Λ. (2020) · Biomolecules · doi:10.3390/biom10050694 · Cited by 40
Properties of the docosahexaenoic acid-producer Schizochytrium mangrovei Sk-02: effects of glucose, temperature and salinity and their interaction
Unagul, P., Assantachai, C., Phadungruengluij, S., Suphantharika, M., & Verduyn, C. (2005) · Botanica Marina · doi:10.1515/bot.2005.052 · Cited by 37
Fermentation-Derived Bioactive Components from Seaweeds: Functional Properties and Potential Applications
Chye, F. Y., Ooi, P. W., Ng, S. Y., & Sulaiman, M. R. (2017) · Journal of Aquatic Food Product Technology · doi:10.1080/10498850.2017.1412375 · Cited by 37
Antarctic microorganisms as source of the omega-3 polyunsaturated fatty acids
Bianchi, A. C., Olazábal, L., Torre, A., & Loperena, L. (2014) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-014-1607-2 · Cited by 36
Marine Functional Foods
Freitas, A. C., Pereira, L., Rodrigues, D., CARVALHO, A. P. F. D., Panteleitchouk, T. S. L., Gomes, A., & Duarte, A. C. (2015) · Springer eBooks · doi:10.1007/978-3-642-53971-8_42 · Cited by 36
PHYCOREMEDIATION AND THE POTENTIAL OF SUSTAINABLE ALGAL BIOFUEL PRODUCTION USING WASTEWATER
Fathi, A. A. (2013) · American Journal of Applied Sciences · doi:10.3844/ajassp.2013.189.194 · Cited by 36
Bioactive Compounds in Seafood: Implications for Health and Nutrition
Tufail, T., Ain, H. B. U., Ashraf, J., Mahmood, S., Noreen, S., Ijaz, A., Ikram, A., Arshad, M. T., & Abdullahi, M. A. (2025) · Food Science & Nutrition · doi:10.1002/fsn3.70181 · Cited by 39
A novel bioprocess engineering approach to recycle hydrophilic and hydrophobic waste under high salinity conditions for the production of nutraceutical compounds
Patel, A., Vellosillo, I. D., Rova, U., Μάτσακας, Λ., & Christakopoulos, P. (2021) · Chemical Engineering Journal · doi:10.1016/j.cej.2021.133955 · Cited by 34
Polyunsaturated fatty acid production by marine bacteria
Abdelrazak, A., Ward, A. C., & Glassey, J. (2013) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-013-0936-0 · Cited by 34
Current and potential uses of bioactive molecules from marine processing waste
Suleria, H. A. R., Masci, P. P., Gobe, G. C., & Osborne, S. A. (2015) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.7444 · Cited by 33
Efficient Extraction of a Docosahexaenoic Acid (DHA)-Rich Lipid Fraction from Thraustochytrium sp. Using Ionic Liquids
Zhang, Y., Ward, V. C. A., Dennis, D. A., Plechkova, N. V., Armenta, R. E., & Rehmann, L. (2018) · Materials · doi:10.3390/ma11101986 · Cited by 34
Different carbon and nitrogen sources regulated docosahexaenoic acid (DHA) production of Thraustochytriidae sp. PKU#SW8 through a fully functional polyunsaturated fatty acid (PUFA) synthase gene (pfaB)
Chen, X., He, Y., Ye, H., Xie, Y., Sen, B., Jiao, N., & Wang, G. (2020) · Bioresource Technology · doi:10.1016/j.biortech.2020.124273 · Cited by 34
Year-Round Cultivation of Tetraselmis sp. for Essential Lipid Production in a Semi-Open Raceway System
Lee, W.-K., Ryu, Y.-K., Choi, W.-Y., Kim, T., Park, A., Lee, Y.-J., Jeong, Y., Lee, C., & Kang, D.-H. (2021) · Marine Drugs · doi:10.3390/md19060314 · Cited by 33
Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276
Furlan, V. J. M., MAUS, V. W., Batista, I., & Bandarra, N. M. (2017) · Brazilian Journal of Microbiology · doi:10.1016/j.bjm.2017.01.001 · Cited by 32
Simultaneous photoautotrophic production of DHA and EPA by Tisochrysis lutea and Microchloropsis salina in co-culture
Thurn, A.-L., Stock, A., Gerwald, S., & Weuster‐Botz, D. (2022) · Bioresources and Bioprocessing · doi:10.1186/s40643-022-00612-5 · Cited by 31
Light enhanced the accumulation of total fatty acids (TFA) and docosahexaenoic acid (DHA) in a newly isolated heterotrophic microalga Crypthecodinium sp. SUN
Sun, D., Zhang, Z., Mao, X., Wu, T., Jiang, Y., Liu, J., & Chen, F. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.12.077 · Cited by 31
13C Metabolic Flux Analysis of Enhanced Lipid Accumulation Modulated by Ethanolamine in Crypthecodinium cohnii
Cui, J., Diao, J., Sun, T., Shi, M., Liu, L., Wang, F., Chen, L., & Zhang, W. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00956 · Cited by 31
Marine microorganisms: natural factories for polyunsaturated fatty acid production
Tian, L., Chi, G., Lin, S., Ling, X., & He, N. (2024) · Blue Biotechnology · doi:10.1186/s44315-024-00012-8 · Cited by 32
Modulation of the neurotransmitter systems through the anti-inflammatory and antidepressant-like effects of squalene from Aurantiochytrium sp.
Sasaki, K., Othman, M. B., Ferdousi, F., Yoshida, M., Watanabe, M. M., Tominaga, K., & Isoda, H. (2019) · PLoS ONE · doi:10.1371/journal.pone.0218923 · Cited by 30
A comparative study on the effect of algal and fish oil on viability and cell proliferation of Caco-2 cells
van Beelen, V. A., Roeleveld, J., Mooibroek, H., Sijtsma, L., Bino, R. J., Bosch, D., Rietjens, I. M. C. M., & Alink, G. M. (2006) · Food and Chemical Toxicology · doi:10.1016/j.fct.2006.10.017 · Cited by 30
Self-citation
DHA protects against monosodium urate-induced inflammation through modulation of oxidative stress
Zhang, Y., Liu, L., Sun, D., He, Y., Jiang, Y., Cheng, K., & Chen, F. (2019) · Food & Function · doi:10.1039/c9fo00573k · Cited by 30
Microencapsulation of Docosahexaenoic Acid Algal Oil Using Spray Drying Technology
Shao, W., Pan, X., Liu, X., Teng, F., & Yuan, S. (2018) · Food Technology and Biotechnology · doi:10.17113/ftb.56.01.18.5452 · Cited by 29
Genetic Engineering of Crypthecodinium cohnii to Increase Growth and Lipid Accumulation
Diao, J., Song, X., Zhang, X., Chen, L., & Zhang, W. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00492 · Cited by 30
Blue biotechnology: a vision for future marine biorefineries
Prabha, S. P., Nagappan, S., Rathna, R., Viveka, R., & Nakkeeran, E. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-818996-2.00021-1 · Cited by 29
Fish Oil and Microalga Omega‐3 as Dietary Supplements: A Comparative Study on Cardiovascular Risk Factors in High‐Fat Fed Rats
Haimeur, A., Mimouni, V., Ulmann, L., Martineau, A., Messaouri, H., Pineau‐Vincent, F., Tremblin, G., & Meskini, N. (2016) · Lipids · doi:10.1007/s11745-016-4177-2 · Cited by 28
Docosahexaenoic acid production from the acidic hydrolysate of Jerusalem artichoke by an efficient sugar-utilizing Aurantiochytrium sp. YLH70
Yu, X., Liu, J., Sun, J., Zheng, J., Zhang, Y., & Wang, Z. (2016) · Industrial Crops and Products · doi:10.1016/j.indcrop.2016.01.013 · Cited by 28
The balance of n-6 and n-3 fatty acids in canine, feline, and equine nutrition: exploring sources and the significance of alpha-linolenic acid
Burron, S., Richards, T. L., Krebs, G., Trevizan, L., Rankovic, A., Hartwig, S., Pearson, W., David, W. L., & Shoveller, A. K. K. (2024) · Journal of Animal Science · doi:10.1093/jas/skae143 · Cited by 37
Fatty Acid Profiles and Nutritional Evaluation of Fresh Sweet-Waxy Corn from Three Regions of China
Li, Z., Hong, T., Zhao, Z., Gu, Y., Guo, Y., & Han, J. (2022) · Foods · doi:10.3390/foods11172636 · Cited by 27
Alternative Carbon Sources for Heterotrophic Production of Docosahexaenoic Acid by the Marine Alga Crypthecodinium cohnii
Sijtsma, L., Anderson, A. J., & Ratledge, C. (2010) · Elsevier eBooks · doi:10.1016/b978-1-893997-73-8.50011-6 · Cited by 27
Self-citation
The production of eicosapentaenoic acid by representatives of the genus Mortierella grown on brewers’ spent grain
Jacobs, A., Botha, A., & van Zyl, W. H. (2009) · Biologia · doi:10.2478/s11756-009-0152-1 · Cited by 27
Acid-catalyzed hot-water extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101
Choi, S.-A., Jung, J.-Y., Kim, K., Lee, J., Kwon, J.-H., Kim, S. W., Yang, J.-W., & Park, J. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.03.153 · Cited by 27
Functional ingredients produced by culture of Koliella antarctica
Fogliano, V., Andreoli, C., Martello, A., Caiazzo, M., Lobosco, O., Formisano, F., Carlino, P. A., Meca, G., Graziani, G., Rigano, V. D. M., et al. (2009) · Aquaculture · doi:10.1016/j.aquaculture.2009.11.008 · Cited by 27
Growth, fatty acid profile in major lipid classes and lipid fluidity of Aurantiochytrium mangrovei Sk-02 as a function of growth temperature
Chodchoey, K., & Verduyn, C. (2012) · Brazilian Journal of Microbiology · doi:10.1590/s1517-83822012000100020 · Cited by 27
Progress of metabolic engineering for the production of eicosapentaenoic acid
Jia, Y.-L., Geng, S., Du, F., Xu, Y., Wang, L., Sun, X., Wang, Q., & Li, Q. (2021) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2021.1971621 · Cited by 28
Growth and docosahexaenoic acid production performance of the heterotrophic marine microalgae Crypthecodinium cohnii in the wave‐mixed single‐use reactor CELL ‐tainer
Hillig, F., Porscha, N., Junne, S. G., & Neubauer, P. B. (2014) · Engineering in Life Sciences · doi:10.1002/elsc.201400010 · Cited by 27
Biomass Composition, Lipid Characterization, and Metabolic Profile Analysis of the Fed-Batch Fermentation Process of Two Different Docosahexanoic Acid Producing Schizochytrium sp. Strains
Qu, L., Ren, L., Li, J., Sun, G., Sun, L., Ji, X., Nie, Z.-K., & Huang, H. (2013) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-013-0456-z · Cited by 26
Phycochemistry and bioactivity of cyanobacterial secondary metabolites
Srivastava, R., Prajapati, R., Kanda, T., Yadav, S., Singh, N., Yadav, S., Mishra, R., & Atri, N. (2022) · Molecular Biology Reports · doi:10.1007/s11033-022-07911-2 · Cited by 25
γ‐Linolenic acid production by Arthrospira platensis using different carbon sources
Golmakani, M., Rezaei, K., Mazidi, S., & Razavi, S. H. (2011) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201100264 · Cited by 25
Livers of terrestrial production animals as a source of long‐chain polyunsaturated fatty acids for humans: An alternative to fish?
Gladyshev, M. I., Makhutova, O. N., Gubanenko, G. A., Александровна, Р. Е., Kalachova, G. S., & Sushchik, N. N. (2015) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201400449 · Cited by 24
Optimization of Fatty Acid Determination in Selected Fish and Microalgal Oils
Armenta, R. E., Scott, S. D., Burja, A. M., Radianingtyas, H., & Barrow, C. J. (2009) · Chromatographia · doi:10.1365/s10337-009-1143-7 · Cited by 24
A New Labyrinthulid Isolate That Produces Only Docosahexaenoic Acid
Kumon, Y., Yokoyama, R., Haque, Z., Yokochi, T., Honda, D., & Nakahara, T. (2005) · Marine Biotechnology · doi:10.1007/s10126-005-5098-x · Cited by 22
Algae Biotechnology
Rasul, I., Azeem, F., Siddique, M. H., Muzammil, S., Rasul, A., Munawar, A., Afzal, M., Ali, M. A., & Nadeem, H. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-812360-7.00008-2 · Cited by 24
Experimental evolution of phytoplankton fatty acid thermal reaction norms
O’Donnell, D. R., Du, Z., & Litchman, E. (2019) · Evolutionary Applications · doi:10.1111/eva.12798 · Cited by 23
Biomedical engineering utilizing living photosynthetic cyanobacteria and microalgae: Current status and future prospects
Zhang, T., Liu, D., Zhang, Y., Chen, L., Zhang, W., & Sun, T. (2024) · Materials Today Bio · doi:10.1016/j.mtbio.2024.101154 · Cited by 25
Utilization of cane molasses for docosahexaenoic acid production by Schizochytrium sp. CCTCC M209059
Ren, L., Li, J., Hu, Y., Ji, X., & Huang, H. (2013) · Korean Journal of Chemical Engineering · doi:10.1007/s11814-013-0020-0 · Cited by 23
Ultrasound-assisted in-situ transesterification of wet Aurantiochytrium sp. KRS 101 using potassium carbonate
Sung, M., & Han, J. (2018) · Bioresource Technology · doi:10.1016/j.biortech.2018.03.099 · Cited by 23
Batch kinetics and modeling of DHA production by S. limacinum OUC88
Song, X., Zhang, X., Kuang, C., Zhu, L., & Zhao, X. (2009) · Food and Bioproducts Processing · doi:10.1016/j.fbp.2009.12.004 · Cited by 22
Dietary DHA Oil Supplementation Promotes Ovarian Development and Astaxanthin Deposition during the Ovarian Maturation of Chinese Mitten Crab Eriocheir sinensis
Jiang, X., Pan, K., Yang, Y., Shu‐Chien, A. C., & Wu, X. (2022) · Aquaculture Nutrition · doi:10.1155/2022/9997317 · Cited by 22
Novel squalene-producing thraustochytrids found in mangrove water
Otagiri, M., Khalid, A., Moriya, S., Osada, H., & Takahashi, S. (2017) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2017.1359485 · Cited by 21
Integrated algal and oil palm biorefinery as a model system for bioenergy co-generation with bioproducts and biopharmaceuticals
Abdullah, M. A., & Hussein, H. A. (2021) · Bioresources and Bioprocessing · doi:10.1186/s40643-021-00396-0 · Cited by 19
SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells
González‐Fernández, M. J., Fabrikov, D., Ramos‐Bueno, R. P., Guil‐Guerrero, J. L., & Ortea, I. (2019) · Nutrients · doi:10.3390/nu11122984 · Cited by 19
Sunflower press cake as a substrate for eicosapentaenoic acid production by representatives of the genus Mortierella
Jacobs, A., Botha, A., Reddy, J. K., & van Zyl, W. H. (2010) · BioResources · doi:10.15376/biores.5.2.1232-1243 · Cited by 19
Biomass and docosahexaenoic acid formation by Schizochytrium mangrovei Sk-02 at low salt concentrations
Unagul, P., Assantachai, C., Phadungruengluij, S., Pongsuteeragul, T., Suphantharika, M., & Verduyn, C. (2006) · Botanica Marina · doi:10.1515/bot.2006.023 · Cited by 18
Effects of molten-salt/ionic-liquid mixture on extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101
Choi, S.-A., Jung, J.-Y., Kim, K., Kwon, J.-H., Lee, J., Kim, S. W., Park, J., & Yang, J.-W. (2014) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-014-1197-2 · Cited by 17
Terrestrial Sources of Polyunsaturated Fatty Acids for Aquaculture
Gladyshev, M. I. (2021) · Journal of Ichthyology · doi:10.1134/s0032945221030036 · Cited by 18
Propiedades antiinflamatorias de los ácidos grasos poliinsaturados omega-3. Indicaciones en oftalmología
Pinazo-Duran, M. D., & Boscá, L. (2012) · Archivos de la Sociedad Española de Oftalmología · doi:10.1016/j.oftal.2012.04.003 · Cited by 16
Solid waste reduction of closed recirculated aquaculture systems by secondary culture of detritivorous organisms
Bischoff, A. A. (2007) · Helmholtz Centre for Ocean Research Kiel (GEOMAR) · Cited by 16
High contents of eicosapentaenoic acid and docosahexaenoic acid in the mixotrophic dinoflagellate Paragymnodinium shiwhaense and identification of putative omega-3 biosynthetic genes
Jang, S. H., Jeong, H. J., & Kwon, J. (2017) · Algal Research · doi:10.1016/j.algal.2017.06.020 · Cited by 15
Production of linoleic acid‐rich lipids in molasses‐based medium by oleaginous fungus Galactomyces geotrichum TS61
Altun, R., Esim, N., Aykutoğlu, G., Baltacı, M. Ö., Adıgüzel, A., & Taşkın, M. (2020) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.14518 · Cited by 16
Effects of light intensity, light quality, and illumination period on cell growth, TFA accumulation, and DHA production in Crypthecodinium sp. SUN
Sun, D., Zhang, Z., & Chen, F. (2018) · Journal of Applied Phycology · doi:10.1007/s10811-017-1379-9 · Cited by 14
Identification and quantification of triacylglycerols containing n-3 long-chain polyunsaturated fatty acids in bovine milk
Liu, Z., Moate, P. J., Ezerniks, V., Cocks, B. G., & Rochfort, S. J. (2015) · Journal of Dairy Science · doi:10.3168/jds.2015-9889 · Cited by 14
Docosahexaenoic acid production of the marine microalga Isochrysis galbana cultivated on renewable substrates from food processing waste under CO2 enrichment
Zheng, H., Ge, F., Song, K., ZiXiang, Y., Li, J., Yan, F., Wu, X., Zhang, Q., Liu, Y., & Ruan, R. R. (2022) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2022.157654 · Cited by 16
Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1
Xu, J., Zhu, Y., Li, H., Chen, L., Chen, W., Cui, M., Han, L., Wenyu, H. O. U., & Li, D. (2018) · Electronic Journal of Biotechnology · doi:10.1016/j.ejbt.2018.06.002 · Cited by 14
In Silico Methodologies to Improve Antioxidants’ Characterization from Marine Organisms
Lauritano, C., Montuori, E., Falco, G. D., & Carrella, S. (2023) · Antioxidants · doi:10.3390/antiox12030710 · Cited by 15
Kinetic and Stoichiometric Modeling-Based Analysis of Docosahexaenoic Acid (DHA) Production Potential by Crypthecodinium cohnii from Glycerol, Glucose and Ethanol
Berzins, K., Muiznieks, R., Baumanis, M. R., Strazdiņa, I., Shvirksts, K., Prikule, S., Galvanauskas, V., Pleißner, D., Pentjuss, A., Grūbe, M., et al. (2022) · Marine Drugs · doi:10.3390/md20020115 · Cited by 13
Biological Activities of Marine Products and Nutritional Importance
Pal, D., & Raj, K. (2020) · Advanced structured materials · doi:10.1007/978-3-030-54027-2_17 · Cited by 13
Marine oils from seafood waste
Shahidi, F. (2007) · Elsevier eBooks · doi:10.1533/9781845692087.2.258 · Cited by 13
Investigation of the physiological properties and synthesis of PUFAs from Thraustochytrids and its electrophoretic karyotypes
Anbu, P., Kim, D.-U., Jeh, E.-J., Jeong, Y., & Hur, B. (2007) · Biotechnology and Bioprocess Engineering · doi:10.1007/bf02931091 · Cited by 13
Microbial Oils as Nutraceuticals and Animal Feeds
Galán, B., Santos‐Merino, M., Nogales, J., de la Cruz, F., & García, J. L. (2020) · Springer eBooks · doi:10.1007/978-3-030-15147-8_34 · Cited by 12
Eliminating the accumulation of reactive oxygen species through periodic hypoxic stress control for effective DHA production by Schizochytrium sp
Jia, L., Li, T., Yang, Z., He, T., Ding, J., Li, T., & Huang, A. (2023) · Chemical Engineering Science · doi:10.1016/j.ces.2023.119040 · Cited by 13
Recognizing the Health Benefits of Plant‐Sourced Omega‐3 Stearidonic Acid: Exploring Its Complementary Role to Preformed EPA/DHA
Baker, E. J., Cumberford, G., & Hanaway, P. J. (2025) · Lipids · doi:10.1002/lipd.12452 · Cited by 12
High yield of long-chain polyunsaturated fatty acids by labyrinthulids on soybean lecithin-dispersed agar medium
Kumon, Y., Yokochi, T., & Nakahara, T. (2005) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-005-1978-2 · Cited by 12
Microbial Oils as Nutraceuticals and Animal Feeds
Galán, B., Santos‐Merino, M., Nogales, J., de la Cruz, F., & García, J. L. (2018) · Springer eBooks · doi:10.1007/978-3-319-72473-7_34-1 · Cited by 11
Application of Seaweeds in the Food Industry
García-Sartal, C., Barciela‐Alonso, M. C., & Bermejo‐Barrera, P. (2011) · Wiley eBooks · doi:10.1002/9781119977087.ch34 · Cited by 13
Simultaneous Imaging and Characterization of Polyunsaturated Fatty Acids, Carotenoids, and Microcrystalline Guanine in Single Aurantiochytrium limacinum Cells with Linear and Nonlinear Raman Microspectroscopy
Sasaki, R., Toda, S., Sakamoto, T., Sakuradani, E., & Shigeto, S. (2023) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.3c00302 · Cited by 11
Network pharmacology‒based analysis of marine cyanobacteria derived bioactive compounds for application to Alzheimer’s disease
Xie, R., Chen, F., Ma, Y., Hu, W., Zheng, Q., Cao, J., & Wu, Y. (2023) · Frontiers in Pharmacology · doi:10.3389/fphar.2023.1249632 · Cited by 12
Evaluation of diverse biochemical stimulants to enhance growth, lipid and docosahexaenoic acid (DHA) production of Aurantiochytrium Sp. ATCC PRA-276
Hussain, S. A., Sarker, M. I. M., Yosief, H. O., & Yadav, M. P. (2021) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2021.102122 · Cited by 12
Designer and Functional Food Lipids in Dietary Regimes: Current Trends and Future Prospects
Jala, R. C. R., & Kumar, C. G. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-811446-9.00010-1 · Cited by 12
Application of Jerusalem artichoke and lipid-extracted algae hydrolysate for docosahexaenoic acid production by Aurantiochytrium sp. KRS101
Heo, S.-W., Oh, Y. T., Kim, Z., Chang, Y. K., & Lee, B. (2020) · Journal of Applied Phycology · doi:10.1007/s10811-020-02207-z · Cited by 11
Simple, effective protein extraction method and proteomics analysis from polyunsaturated fatty acids-producing micro-organisms
Ling, X., Guo, J., Zheng, C., Ye, C., Lu, Y., Pan, X., Chen, Z., & Ng, I. (2015) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-015-1467-7 · Cited by 11
Omega-3 LC-PUFA consumption is now recommended for women of childbearing age and during pregnancy to protect against preterm and early preterm birth: implementing this recommendation in a sustainable manner
Baker, E. J., Calder, P. C., Kermack, A., Brown, J. E., Mustapha, M., Kitson-Reynolds, E., & Garvey, J. (2024) · Frontiers in Nutrition · doi:10.3389/fnut.2024.1502866 · Cited by 10
Fatty acid composition and docosahexaenoic acid (DHA) content of the heterotrophic dinoflagellate Oxyrrhis marina fed on dried yeast: compared with algal prey
Yoon, E. Y., Park, J., Jeong, H. J., & Rho, J. (2017) · ALGAE · doi:10.4490/algae.2017.32.3.5 · Cited by 10
Bioactive Metabolites and Value-Added Products from Marine Macroalgae
Rengasamy, K. R. R., Kulkarni, M. G., Stirk, W. A., & Staden, J. V. (2013) · Springer eBooks · doi:10.1007/978-1-4614-9590-1_21 · Cited by 10
Microbial production of omega-3 polyunsaturated fatty acids
Verma, M. L., Kishor, K., Sharma, D., Kumar, S., & Sharma, K. D. (2019) · Elsevier eBooks · doi:10.1016/b978-0-444-64323-0.00010-2 · Cited by 11
Biological Carbon Recovery from Sugar Refinery Washing Water into Microalgal DHA: Medium Optimization and Stress Induction
Moon, M., Park, W.-K., Suh, W. I., Chang, Y. K., & Lee, B. (2019) · Scientific Reports · doi:10.1038/s41598-019-56406-x · Cited by 9

Authors: Sijtsma, L., & de Swaaf, M. E.
Affiliations: Wageningen University & Research (NL)
Corresponding author: Sijtsma, L.
ORCID: Sijtsma, L.
Venue: Applied Microbiology and Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2004-01-22
Volume: 64
Issue: 2
Pages: 146-153
ISSN: 0175-7598 (ISSN-L)
1432-0614
DOI: 10.1007/s00253-003-1525-y
OpenAlex: W2093047642
Cited by: 278
References: 60
Citations by year: 2026: 1 · 2025: 9 · 2024: 8 · 2023: 7 · 2022: 9 · 2021: 18 · 2020: 19 · 2019: 15 · 2018: 14 · 2017: 15 · 2016: 9 · 2015: 22 · 2014: 22 · 2013: 20 · 2012: 20
FWCI: 6.52
Type: journal article
Language: en
Open access: green
research.wur.nl
Licence: www.springer.com/tdm (tdm)
Locations: Applied Microbiology and Biotechnology (publishedVersion)
PubMed (publishedVersion)
Socio-Environmental Systems Modeling (acceptedVersion)
Data Archiving and Networked Services (DANS) (submittedVersion)
Topics: Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords: docosahexaenoic acid, omega-3 fatty acids, polyunsaturated fatty acids, marine microorganisms, heterotrophic cultivation, fish oil alternatives
MeSH terms: Animals, Biotechnology, Dinoflagellida, Docosahexaenoic Acids, Fungi, Bioreactors, Eukaryota

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Biotechnological production and applications of the ω-3 polyunsaturated fatty acid docosahexaenoic acid

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks