Physiological and molecular analysis of carbon source supplementation and pH stress-induced lipid accumulation in the marine diatom Phaeodactylum tricornutum
Abstract
A detailed physiological and molecular analysis of lipid accumulation under a suite of conditions including nitrogen limitation, alkaline pH stress, bicarbonate supplementation, and organic acid supplementation was performed on the marine diatom Phaeodactylum tricornutum. For all tested conditions, nitrogen limitation was a prerequisite for lipid accumulation and the other culturing strategies only enhanced accumulation highlighting the importance of compounded stresses on lipid metabolism. Volumetric lipid levels varied depending on condition; the observed rankings from highest to lowest were for inorganic carbon addition (15 mM bicarbonate), organic acid addition (15 carbon mM acetate), and alkaline pH stress (pH 9.0). For all lipid-accumulating cultures except acetate supplementation, a common series of physiological steps were observed. Upon extracellular nitrogen exhaustion, culture growth continued for approximately 1.5 cell doublings with decreases in specific protein and photosynthetic pigment content. As nitrogen limitation arrested cell growth, carbohydrate content decreased with a corresponding increase in lipid content. Addition of the organic carbon source acetate appeared to activate alternative metabolic pathways for lipid accumulation. Molecular level data on more than 50 central metabolism transcripts were measured using real-time PCR. Analysis of transcripts suggested the central metabolism pathways associated with bicarbonate transport, carbonic anhydrases, and C4 carbon fixations were important for lipid accumulation. Transcriptomic data also suggested that repurposing of phospholipids may play a role in lipid accumulation. This study provides a detailed physiological and molecular-level foundation for improved understanding of diatom nutrient cycling and contributes to a metabolic blueprint for controlling lipid accumulation in diatoms.
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Porphyridium cruentum produces high quantities of vitamins E and C, as well as β-carotene (vitamin A).
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From The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products (2018) · cites this paper as [204]
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- Authors
- Mus, F., Toussaint, J.-P., Cooksey, K. E., Fields, M. W., Gerlach, R., Peyton, B. M., & Carlson, R. P.
- Affiliations
- Montana State University (US)
- Corresponding author
- Mus, Florence
- Carlson, Ross P.
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2013-03-06
- Volume
- 97
- Issue
- 8
- Pages
- 3625-3642
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2142110641
- Cited by
- 132
- References
- 87
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 6 · 2023: 12 · 2022: 13 · 2021: 7 · 2020: 15 · 2019: 14 · 2018: 13 · 2017: 7 · 2016: 10 · 2015: 11 · 2014: 11 · 2013: 5
- FWCI
- 4.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Applied Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Montana State University ScholarWorks (Montana State University) (submittedVersion)
- Funders
- Air Force Office of Scientific Research
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Phaeodactylum tricornutum, lipid accumulation, nitrogen limitation, sodium bicarbonate supplementation, acetate supplementation, carbon source supplementation, central carbon metabolism, carbonic anhydrase, bicarbonate transport
- MeSH terms
- Alkalies, Carbon, Carboxylic Acids, Hydrogen-Ion Concentration, Nitrogen, Stress, Physiological, Diatoms, Gene Expression Profiling, Lipid Metabolism, Metabolic Networks and Pathways, Aquatic Organisms, Real-Time Polymerase Chain Reaction
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Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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