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Cited by 67Probiotics and Fermented FoodsReproductive tract infections researchWastewater Treatment and Nitrogen Removal

Evaluation of nitric oxide production by lactobacilli

Xu, J., Verstraete, W.·2001·Applied Microbiology and Biotechnology
DOI 10.1007/s002530100616
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Abstract

Six strains of Lactobacillus fermentum and Lactobacillus plantarum were investigated for nitric oxide (NO) production. First, the potential presence of NO synthase was examined. None of the strains of L. fermentum and L. plantarum examined produced NO from L-arginine under aerobic conditions. Interestingly, all L. fermentum strains expressed strong L-arginine deiminase activity. All L. fermentum strains produced NO in MRS broth, but the NO was found to be chemically derived from nitrite, which was produced by L. fermentum from nitrate present in the medium. Indeed all L. fermentum strains express nitrate reductase under anaerobic conditions. Moreover, one strain, L. fermentum LF1, had nitrate reductase activity under aerobic conditions. It was also found that L. fermentum strains JCM1173 and LF1 possessed ammonifying nitrite reductase. The latter strain also had denitrifying nitrite reductase activity at neutral pH under both anaerobic and aerobic conditions. The LF1 strain is thus capable of biochemically converting nitrate to NO. NO and nitrite produced from nitrate by lactobacilli may constitute a potential antimicrobial mechanism. studied in a rat acute liver injury model (Adawi et al. 1997). The results indicate that Lactobacillus plantarum DSM 9842 may possess NOS (Adawi et al. 1997). However, NO production from L-arginine has not been investigated in pure cultures of L. plantarum. According to the results of a 15N enrichment experiment, traces of (NO2-+NO3-)-N (total o…

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  • Several following studies have proposed different mechanisms of bacterial NO formation independent of respiratory denitrification, such as arginine-dependent bacterial NOS activity, DNRA, and non-enzymatic processes.

    “However, several following studies on bacterial NO formation have proposed different mechanisms independent of respiratory denitrification such as arginine dependent bacterial NOS enzymatic activity, DNRA and non-enzymatic processes [15,32–34].”

    From Nitrate reduction to nitrite, nitric oxide and ammonia by gut bacteria under physiological conditions (2015) · cites this paper as [32]

  • Respiratory denitrification can generate small but relevant amounts of NO as an intermediate product and has been implicated in bacterial NO production in the gut.

    “Respiratory denitrification can also generate small but relevant amounts of NO as an intermediate product and has been implicated in the bacterial NO production in the gut [32,33].”

    From Nitrate reduction to nitrite, nitric oxide and ammonia by gut bacteria under physiological conditions (2015) · cites this paper as [32]

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Authors
Xu, J., & Verstraete, W.
Affiliations
Ghent University (BE)
Corresponding author
Verstraete, W.
ORCID
Venue
Applied Microbiology and Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2001-08-01
Volume
56
Issue
3-4
Pages
504-507
ISSN
  • 0175-7598 (ISSN-L)
  • 1432-0614
DOI
10.1007/s002530100616
OpenAlex
W1996138611
Cited by
67
References
15
Citations by year
2025: 3 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 2 · 2017: 3 · 2016: 5 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 5
FWCI
3.05
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Applied Microbiology and Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
Keywords
nitric oxide production, Lactobacillus fermentum, Lactobacillus plantarum, nitric oxide synthase, nitrate reductase, nitrite reductase, denitrification, nitrite, antimicrobial mechanisms, lactic acid bacteria, 15N labeling, nitrification
MeSH terms
Arginine, Culture Media, Lactobacillus, Nitrates, Nitric Oxide, Nitrite Reductases, Oxidation-Reduction, Nitric Oxide Synthase

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