Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells
Abstract
A highly efficient system was developed for the expression of foreign genes in Chlorella ellipsoidea cells. The effect of five promoters on the expression efficiency of beta-glucuronidase (GUS) gene was evaluated by transient expression of the UidA gene. Among these promoters, Ubiquitin-omega was found to be the most efficient and was selected to drive the expression of foreign genes in Chlorella cells. A gene encoding the mature rabbit neutrophil peptide-1 (NP-1) was introduced into the cells. Integration of the gene for NP-1 into the Chlorella genome was confirmed by PCR and Southern blot analysis. In, vitro anti-microbial testes demonstrated the expression of biologically active NP-1 by the transgenic Chlorella cells.
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The Chlorella ellipsoidea nuclear genome was engineered to produce rabbit neutrophil peptide-1, which proved to be effective against human pathogens in vitro.
“The Chlorella ellipsoidea nuclear genome was engineered to produce rabbit neutrophil peptide-1, which proved to be effective agent against human pathogens in vitro [79].”
From Biofuels from algae: Challenges and potential (2010) · cites this paper as [79]
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Swart, C. (2013) · SUNScholar (Stellenbosch University) · Cited by 1 · see citing works
Other Biopharmaceuticals Produced in Algae
Rosales‐Mendoza, S. (2016) · Springer eBooks · doi:10.1007/978-3-319-32232-2_7 · Cited by 1 · see citing works
31 Lipid Biotechnology and Biochemistry
Anankanbil, S., Suo, F., Jensen, P. R., & Guo, Z. (2017) · CRC Press eBooks · doi:10.1201/9781315151854-32 · Cited by 1 · see citing works
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The Development Of Microalgae As A Bioreactor System For The Production Of Recombinant Proteins
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Investigation on Electroporation: Mediated Transformation of Chlorella ellipsoidea
Zuo, Z., Qin, F., Zhang, Y., et al. (2013) · Lecture notes in electrical engineering · doi:10.1007/978-3-642-37916-1_28
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Unveiling microbial and microalgal chassis for therapeutic and diagnostic protein expression
Bhandari, L., Kulkarni, S., & Prakash, G. (2026) · Biotechnology Progress · doi:10.1002/btpr.70098
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- Authors
- Chen, Y., Wang, Y., Sun, Y., Zhang, L., & Li, W.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Li, Wenbin
- Venue
- Current Genetics
- Published
- 2001-07
- Volume
- 39
- Issue
- 5-6
- Pages
- 365-370
- ISSN
- 0172-8083 (ISSN-L)
- 1432-0983
- OpenAlex
- W2063036391
- Cited by
- 95
- References
- 0
- Citations by year
- 2026: 4 · 2024: 2 · 2023: 1 · 2021: 3 · 2020: 8 · 2019: 5 · 2017: 8 · 2016: 14 · 2015: 7 · 2014: 6 · 2013: 5 · 2012: 5
- FWCI
- 1.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Current Genetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- Chlorella ellipsoidea, ubiquitin promoter, GUS reporter, transgenic algae, antimicrobial activity, foreign gene integration
- MeSH terms
- Animals, Anti-Infective Agents, Chlorella, Cloning, Molecular, DNA, Gene Expression Regulation, Glucuronidase, Rabbits, Blotting, Southern, Polymerase Chain Reaction, alpha-Defensins
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