Cited by 31Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionPlant tissue culture and regeneration

Nuclear transformation and functional gene expression in the oleaginous microalga Monoraphidium neglectum

Jaeger, Daniel, Hübner, Wolfgang, Huser, Thomas, Mussgnug, Jan H., Kruse, Olaf·2017·Journal of Biotechnology

Abstract

Photosynthetic microalgae hold great promise as non-food feedstocks for the sustainable production of a range of bio-products and genetic engineering is an increasingly important strategy to improve the natural cellular features. For the vast majority of microalgal strains, however, genetic engineering is not yet possible and the establishment of efficient genetic tools for a broad range of microalgal species is an important task to reach biotechnological success. Stable DNA transformation is one of the crucial steps for most genetic engineering approaches. In this context, we report the first successful and stable nuclear genetic transformation of the biotechnologically promising oleaginous microalga M. neglectum. Transformation was achieved by electroporation and the efficiency could progressively be improved by implementation of an additional cell pretreatment step, aiming at weakening the rigid natural cell wall. Furthermore, a first reporter transgene was established for M. neglectum. As a result of this work, genetic engineering strategies now become accessible which are required to successfully establish M. neglectum as a novel host for biotechnological applications.

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Crossref · 10.1016/j.jbiotec.2017.03.011

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Authors
Jaeger, D., Hübner, W., Huser, T., Mussgnug, J. H., & Kruse, O.
Affiliations
Bielefeld University (DE)
Corresponding author
Kruse, Olaf
Venue
Journal of Biotechnology
Publisher
Elsevier BV
Published
2017-05
Volume
249
Pages
10-15
ISSN
0168-1656 (ISSN-L)
OpenAlex
W2596988300
Cited by
31
References
58
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 2
FWCI
0.95
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
  • PUB – Publications at Bielefeld University (Bielefeld University) (submittedVersion)
  • Publikationen an der Universität Bielefeld (Universität Bielefeld) (submittedVersion)
Funders
German state North Rhine-Westphalia
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
Keywords
oleaginous microalgae, nuclear transformation, electroporation, reporter transgenes, photosynthetic microalgae, non-edible feedstock
MeSH terms
Chlorophyta, Biotechnology, Luminescent Proteins, Plasmids, Recombinant Proteins, Transformation, Genetic, Electroporation, Microalgae

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