Nuclear transformation and functional gene expression in the oleaginous microalga Monoraphidium neglectum
Abstract
Photosynthetic microalgae hold great promise as non-food feedstocks for the sustainable production of a range of bio-products and genetic engineering is an increasingly important strategy to improve the natural cellular features. For the vast majority of microalgal strains, however, genetic engineering is not yet possible and the establishment of efficient genetic tools for a broad range of microalgal species is an important task to reach biotechnological success. Stable DNA transformation is one of the crucial steps for most genetic engineering approaches. In this context, we report the first successful and stable nuclear genetic transformation of the biotechnologically promising oleaginous microalga M. neglectum. Transformation was achieved by electroporation and the efficiency could progressively be improved by implementation of an additional cell pretreatment step, aiming at weakening the rigid natural cell wall. Furthermore, a first reporter transgene was established for M. neglectum. As a result of this work, genetic engineering strategies now become accessible which are required to successfully establish M. neglectum as a novel host for biotechnological applications.
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In Monoraphidium neglectum, nuclear genetic transformation targeting biotechnological application enhanced biotechnological potential.
“| 6 | Monoraphidium neglectum | 69.71 | Nuclear genetic transformation | Recommended for biotechnological application | Gene knockout confirmation, growth phenotype assessment | Endogenous promoters or heterologous expression constructs | Enhanced biotechnological potential | Bioremediation, pharmaceuticals | [94] |”
From A Holistic Approach to Circular Bioeconomy Through the Sustainable Utilization of Microalgal Biomass for Biofuel and Other Value-Added Products (2024) · cites this paper as [94]
Cites
Chisti, Y. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.02.001 · Cited by 9,524 · see citing works
Microalgae for biodiesel production and other applications: A review
Mata, T. M., Martins, A. A., & Caetano, N. S. (2010) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2009.07.020 · Cited by 5,685 · see citing works
Sheehan, J., Dunahay, T., Benemann, J., & Roessler, P. (1998) · doi:10.2172/15003040 · Cited by 1,753 · see citing works
(1989) · Elsevier eBooks · doi:10.1016/c2009-0-02778-0 · Cited by 1,166 · see citing works
Guide to Electroporation and Electrofusion
Chang, D. C., Saunders, J. A., Chassy, B. M., & Sowers, A. E. (1992) · Elsevier eBooks · doi:10.1016/c2009-0-21564-9 · Cited by 640 · see citing works
Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana
Radakovits, R., Jinkerson, R. E., Fuerstenberg, S. I., Tae, H., Settlage, R. E., Boore, J. L., & Posewitz, M. C. (2012) · Nature Communications · doi:10.1038/ncomms1688 · Cited by 520 · see citing works
Kremers, G., Goedhart, J., van Munster, E. B., & Gadella, T. W. J. (2006) · Biochemistry · doi:10.1021/bi0516273 · Cited by 501 · see citing works
High-Efficiency Transformation of Chlamydomonas reinhardtii by Electroporation
Shimogawara, K., Fujiwara, S., Grossman, A. R., & Usuda, H. (1998) · Genetics · doi:10.1093/genetics/148.4.1821 · Cited by 488 · see citing works
Vieler, A., Wu, G., Tsai, C.-H., Bullard, B., Cornish, A. J., Harvey, C., Reca, I.-B., Thornburg, C., Achawanantakun, R., Buehl, C. J., et al. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1003064 · Cited by 458 · see citing works
High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.
Kilian, O., Benemann, C. S. E., Niyogi, K., & Vick, B. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1105861108 · Cited by 421 · see citing works
Fundamentals of electroporative delivery of drugs and genes
Neumann, E., Kakorin, S., & Tœnsing, K. (1999) · Bioelectrochemistry and Bioenergetics · doi:10.1016/s0302-4598(99)00008-2 · Cited by 402 · see citing works
Membrane electroporation theories: a review
Chen, C., Smye, S. W., Robinson, M. P., & Evans, J. A. (2006) · Medical & Biological Engineering & Computing · doi:10.1007/s11517-005-0020-2 · Cited by 382 · see citing works
Wu, S., & Letchworth, G. J. (2004) · BioTechniques · doi:10.2144/04361dd02 · Cited by 376 · see citing works
Sizova, I. A., Fuhrmann, M., & Hegemann, P. (2001) · Gene · doi:10.1016/s0378-1119(01)00616-3 · Cited by 372 · see citing works
Electropermeabilization of Mammalian Cells to Macromolecules: Control by Pulse Duration
Rols, M., & Teissié, J. (1998) · Biophysical Journal · doi:10.1016/s0006-3495(98)74060-3 · Cited by 348 · see citing works
DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins
Baek, K., Kim, D. H., Jeong, J., Sim, S. J., Melis, A., Kim, J., Jin, E., & Bae, S. (2016) · Scientific Reports · doi:10.1038/srep30620 · Cited by 342 · see citing works
Designer diatom episomes delivered by bacterial conjugation
Karas, B. J., Diner, R. E., Lefebvre, S. C., McQuaid, J. B., Phillips, A. P. R., Noddings, C. M., Brunson, J. K., Valas, R. E., Deerinck, T. J., Jablanovic, J., et al. (2015) · Nature Communications · doi:10.1038/ncomms7925 · Cited by 322 · see citing works
Xue, J., Niu, Y.-F., Huang, T., Yang, W.-D., Liu, J.-S., & Li, H.-Y. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2014.10.002 · Cited by 321 · see citing works
Berthold, P. R., Schmitt, R., & Mages, W. (2002) · Protist · doi:10.1078/14344610260450136 · Cited by 287 · see citing works
Escoffre, J., Portet, T., Wasungu, L., Teissié, J., Dean, D. S., & Rols, M. (2008) · Molecular Biotechnology · doi:10.1007/s12033-008-9121-0 · Cited by 257 · see citing works
Minoda, A., Sakagami, R., Yagisawa, F., Kuroiwa, T., & Tanaka, K. (2004) · Plant and Cell Physiology · doi:10.1093/pcp/pch087 · Cited by 243 · see citing works
Transgene Expression in Microalgae—From Tools to Applications
Doron, L., Segal, N., & Shapira, M. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.00505 · Cited by 235 · see citing works
Establishing Chlamydomonas reinhardtii as an industrial biotechnology host
Scaife, M. A., Nguyen, G. T. D. T., Rico, J. J. R., Lambert, D., Helliwell, K. E., & Smith, A. G. (2015) · The Plant Journal · doi:10.1111/tpj.12781 · Cited by 228 · see citing works
Molecular techniques to interrogate and edit the Chlamydomonas nuclear genome
Jinkerson, R. E., & Jonikas, M. C. (2015) · The Plant Journal · doi:10.1111/tpj.12801 · Cited by 162 · see citing works
Lauersen, K. J., Kruse, O., & Mussgnug, J. H. (2015) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6354-7 · Cited by 146 · see citing works
Self-citationGenomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.
Fan, J., Ning, K., Zeng, X., Luo, Y., Wang, D., Hu, J., Li, J., Xu, H., Huang, J., Wan, M., et al. (2015) · PLANT PHYSIOLOGY · doi:10.1104/pp.15.01174 · Cited by 144 · see citing works
High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation
Zhang, C., & Hu, H. (2013) · Marine Genomics · doi:10.1016/j.margen.2013.10.003 · Cited by 143 · see citing works
Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal
Yamano, T., Iguchi, H., & Fukuzawa, H. (2013) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2012.12.020 · Cited by 142 · see citing works
Sukhorukov, V. L., Mussauer, H., & Zimmermann, U. (1998) · The Journal of Membrane Biology · doi:10.1007/s002329900387 · Cited by 139 · see citing works
Selective and asymmetric molecular transport across electroporated cell membranes.
Tekle, E., Astumian, R. D., & Chock, P. B. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.24.11512 · Cited by 135 · see citing works
Highly Efficient Transformation of the DiatomPhaeodactylum tricornutumby Multi-Pulse Electroporation
Miyahara, M., Aoi, M., Inoue‐Kashino, N., Kashino, Y., & Ifuku, K. (2013) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.120936 · Cited by 127 · see citing works
Bogen, C., Al-Dilaimi, A., Albersmeier, A., Wichmann, J., Grundmann, M., Rupp, O., Lauersen, K. J., Blifernez-Klassen, O., Kalinowski, J., Goesmann, A., et al. (2013) · BMC Genomics · doi:10.1186/1471-2164-14-926 · Cited by 108 · see citing works
Self-citationRNA i‐based targeted gene knockdown in the model oleaginous microalgae Nannochloropsis oceanica
Wei, L., Yi, X., Wang, Q., Yang, J., Hu, H., & Xu, J. (2016) · The Plant Journal · doi:10.1111/tpj.13411 · Cited by 108 · see citing works
Electrotransformation of Chlorella vulgaris
Chow, K.-C., & Tung, W. L. (1999) · Plant Cell Reports · doi:10.1007/s002990050660 · Cited by 103 · see citing works
Establishment of an efficient genetic transformation system in Scenedesmus obliquus
Guo, S.-L., Zhao, X., Tang, Y. Z., Wan, C., Alam, M. A., Ho, S., Bai, F., & Chang, J. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.10.020 · Cited by 100 · see citing works
Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli
Rao, R. N., Allen, N. E., Hobbs, J. N., Alborn, W. E., Kirst, H. A., & Paschal, J. W. (1983) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.24.5.689 · Cited by 99 · see citing works
Identification of Monoraphidium contortum as a promising species for liquid biofuel production
Bogen, C., Klassen, V., Wichmann, J., Russa, M. L., Doebbe, A., Grundmann, M., Uronen, P., Kruse, O., & Mussgnug, J. H. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.01.164 · Cited by 97 · see citing works
Self-citationChlamydomonas (Chlorophyceae) colony PCR
Cao, M., Fu, Y., Guo, Y., & Pan, J. (2009) · PROTOPLASMA · doi:10.1007/s00709-009-0036-9 · Cited by 97 · see citing works
Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells
Chen, Y., Wang, Y., Sun, Y., Zhang, L., & Li, W. (2001) · Current Genetics · doi:10.1007/s002940100205 · Cited by 95 · see citing works
Escoffre, J., Portet, T., Favard, C., Teissié, J., Dean, D. S., & Rols, M. (2010) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2010.10.009 · Cited by 93 · see citing works
Overview of Electroporation and Electrofusion
Chang, D. C., Saunders, J. A., Chassy, B. M., & Sowers, A. E. (1992) · Elsevier eBooks · doi:10.1016/b978-0-08-091727-6.50004-6 · Cited by 80 · see citing works
Genetic tools and techniques for Chlamydomonas reinhardtii
Mussgnug, J. H. (2015) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-015-6698-7 · Cited by 80 · see citing works
Self-citationLi, F., Gao, D., & Hu, H. (2014) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2014.905184 · Cited by 76 · see citing works
Genetic engineering of algal chloroplasts: progress and prospects
Purton, S., Szaub, J. B., Wannathong, T., Young, R., & Economou, C. K. (2013) · Russian Journal of Plant Physiology · doi:10.1134/s1021443713040146 · Cited by 74 · see citing works
Schmollinger, S., Strenkert, D., & Schroda, M. (2010) · Current Genetics · doi:10.1007/s00294-010-0304-4 · Cited by 74 · see citing works
Prediction and optimization of gene transfection and drug delivery by electroporation
Canatella, P., & Prausnitz, M. R. (2001) · Gene Therapy · doi:10.1038/sj.gt.3301547 · Cited by 63 · see citing works
Harris, E. H. (1989) · Elsevier eBooks · doi:10.1016/b978-0-12-326880-8.50007-9 · Cited by 63 · see citing works
Chang, D. C. (1992) · Elsevier eBooks · doi:10.1016/b978-0-08-091727-6.50005-8 · Cited by 53 · see citing works
Levitan, O., Dinamarca, J., Zelzion, E., Gorbunov, M. Y., & Falkowski, P. G. (2015) · The Plant Journal · doi:10.1111/tpj.13052 · Cited by 50 · see citing works
Jaeger, D., Pilger, C., Hachmeister, H., Oberländer, E., Wördenweber, R., Wichmann, J., Mussgnug, J. H., Huser, T., & Kruse, O. (2016) · Scientific Reports · doi:10.1038/srep35340 · Cited by 47 · see citing works
Self-citationDíaz‐Santos, E., Vilà, M., Vigara, J., & León, R. (2015) · Journal of Applied Phycology · doi:10.1007/s10811-015-0706-2 · Cited by 43 · see citing works
Sun, Y., Gao, X.-S., Li, Q., Zhang, Q., & Xu, Z. (2006) · Gene · doi:10.1016/j.gene.2006.03.018 · Cited by 43 · see citing works
Radakovits, R., Jinkerson, R. E., Fuerstenberg, S. I., Tae, H., Settlage, R. E., Boore, J. L., & Posewitz, M. C. (2013) · Nature Communications · doi:10.1038/ncomms3356 · Cited by 35 · see citing works
Genomic Transformation of the Picoeukaryote Ostreococcus tauri
Gerben Van Ooijen, A., Knox, K., Kis, K., Bouget, F., & Millar, A. J. (2012) · Journal of Visualized Experiments · doi:10.3791/4074 · Cited by 35 · see citing works
Zorin, B., Grundman, O., Khozin‐Goldberg, I., Leu, S., Shapira, M., Kaye, Y., Tourasse, N. J., Vallon, O., & Boussiba, S. (2014) · PLoS ONE · doi:10.1371/journal.pone.0105223 · Cited by 33 · see citing works
Stable nuclear transformation of the oleaginous microalga Neochloris oleoabundans by electroporation
Chungjatupornchai, W., Kitraksa, P., & Fa-aroonsawat, S. (2015) · Journal of Applied Phycology · doi:10.1007/s10811-015-0594-5 · Cited by 26 · see citing works
Thanh, T. T. V., Quynh, V. T., Omar, H., Abdullah, M. P., & Napis, S. B. (2012) · International Journal of Molecular Sciences · doi:10.3390/ijms13032676 · Cited by 11 · see citing works
Flagged first, then by citations
1 of the 58 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Kumar, G., Shekh, A., Jakhu, S., Sharma, Y., Kapoor, R., & Sharma, T. R. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00914 · Cited by 315 · see citing works
Baier, T., Wichmann, J., Kruse, O., & Lauersen, K. J. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky532 · Cited by 177 · see citing works
Self-citationAdvantages of Heterotrophic Microalgae as a Host for Phytochemicals Production
Jareonsin, S., & Pumas, C. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.628597 · Cited by 100 · see citing works
Ezhumalai, G., Arun, M., Manavalan, A., Rajkumar, R., & Heese, K. (2024) · Microbial Ecology · doi:10.1007/s00248-024-02376-1 · Cited by 99 · see citing works
Eukaryotic microalgae as hosts for light-driven heterologous isoprenoid production
Lauersen, K. J. (2018) · Planta · doi:10.1007/s00425-018-3048-x · Cited by 99 · see citing works
Landscape of microalgae omics and metabolic engineering research for strain improvement: An overview
Stavridou, E., Karapetsi, L., Nteve, G., Tsintzou, G. P., Chatzikonstantinou, M., Tsaousi, M., Martı́nez, Á. T., Flores, P., Merino, M., Dobrovic, L., et al. (2024) · Aquaculture · doi:10.1016/j.aquaculture.2024.740803 · Cited by 74 · see citing works
Gene Delivery Technologies with Applications in Microalgal Genetic Engineering
Gutiérrez, S., & Lauersen, K. J. (2021) · Biology · doi:10.3390/biology10040265 · Cited by 68 · see citing works
Jaeger, D., Winkler, A., Mussgnug, J. H., Kalinowski, J., Goesmann, A., & Kruse, O. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0882-1 · Cited by 65 · see citing works
Self-citationRanjbar, S., & Malcata, F. X. (2022) · Fuel · doi:10.1016/j.fuel.2022.124567 · Cited by 61 · see citing works
Methodological review of genetic engineering approaches for non-model algae
Mosey, M., Douchi, D., Knoshaug, E. P., & Laurens, L. M. L. (2021) · Algal Research · doi:10.1016/j.algal.2021.102221 · Cited by 51 · see citing works
Engineering microalgae: transition from empirical design to programmable cells
Lu, Y., Zhang, X., Gu, X., Lin, H., & Melis, A. (2021) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2021.1917507 · Cited by 52 · see citing works
Alishah Aratboni, H., Rafiei, N., Mehdizadeh Allaf, M., Abedini, S., Naseema Rasheed, R., Seif, A., Barati, B., Wang, S., & Morones-Ramírez, J. R. (2023) · Algal Research · doi:10.1016/j.algal.2023.103019 · Cited by 43 · see citing works
High efficiency transformation by electroporation of the freshwater alga Nannochloropsis limnetica
Chen, Y., & Hu, H. (2019) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-019-2695-9 · Cited by 31 · see citing works
Nuclear transformation of the versatile microalga Euglena gracilis
Khatiwada, B., Kautto, L., Sunna, A., Sun, A., & Nevalainen, H. K. M. (2018) · Algal Research · doi:10.1016/j.algal.2018.11.022 · Cited by 30 · see citing works
Electroporation for microalgal biofuels: a review
Chen, Z., & Lee, W. G. (2019) · Sustainable Energy & Fuels · doi:10.1039/c9se00087a · Cited by 22 · see citing works
Current Status and Perspective on the Use of Viral-Based Vectors in Eukaryotic Microalgae
Bolaños−Martínez, O. C., Mahendran, G., Rosales‐Mendoza, S., & Vimolmangkang, S. (2022) · Marine Drugs · doi:10.3390/md20070434 · Cited by 21 · see citing works
Kato, Y., & Hasunuma, T. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-981-15-7360-6_10 · Cited by 20 · see citing works
Prasad, B., Lein, W., General, T., Lindenberger, C. P., Buchholz, R., & Vadakedath, N. (2018) · Photosynthesis Research · doi:10.1007/s11120-018-0587-8 · Cited by 19 · see citing works
Lee, J.-W., Lee, M., Ha, J.-S., Kim, D., Jin, E., Lee, H., & Oh, H. (2020) · Scientific Reports · doi:10.1038/s41598-020-70503-2 · Cited by 17 · see citing works
Enhanced Lipid Productivity in Low-Starch Mutant of Monoraphidium sp. for Biodiesel Production
Lin, Y., Zhang, Y., Yang, F., Li, C., Ling, H., Ping, W., & Ge, J. (2022) · BioEnergy Research · doi:10.1007/s12155-022-10443-z · Cited by 15 · see citing works
Venkanna, D., Südfeld, C., Baier, T., Homburg, S. V., Patel, A. V., Wobbe, L., & Kruse, O. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01347 · Cited by 14 · see citing works
Self-citationPrasad, B., Lein, W., Lindenberger, C. P., Buchholz, R., & Vadakedath, N. (2018) · Journal of Applied Phycology · doi:10.1007/s10811-018-1617-9 · Cited by 8 · see citing works
Lämmermann, N., Wulf, D., Chang, K. S., Wichmann, J., Jang, J.-H., Jin, E., Bräutigam, A., Wobbe, L., & Kruse, O. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.02.10.942334 · Cited by 8 · see citing works
Self-citationDevelopment and research progress of microalgae as a production platform for antimicrobial peptides
Wang, Q., Ma, Y., Sun, F., Wang, K., Ma, J., Zhu, B., Cao, K., Shao, Y., Cui, Y., Zhang, H., et al. (2025) · Journal of Applied Phycology · doi:10.1007/s10811-025-03472-6 · Cited by 7 · see citing works
Nesamma, A. A., Makaranga, A., Nag, B., Mariamenatu, A. H., Abrha, G. T., & Jutur, P. P. (2023) · Elsevier eBooks · doi:10.1016/b978-0-443-13927-7.00010-4 · Cited by 3 · see citing works
Algal genomics tools: technological updates and progress
Kumar, N., Kar, S., Srivastava, A., Banerjee, C., & Shukla, P. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-95332-0.00008-9 · Cited by 2 · see citing works
Metabolic engineering and molecular biotechnology of microalgae for fuel production
Fang, S. (2019) · Elsevier eBooks · doi:10.1016/b978-0-444-64192-2.00005-6
Microalgae as biofuel: current perspectives and technological progress
Gupta, S., & Ahirwar, S. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-95332-0.00011-9
Singarapu, R. K., Reddy, B. R. B., & Rajesh, M. P. (2023) · Journal of Applied and Natural Science · doi:10.31018/jans.v15i3.4858
Singh, D., Das, A., & Bauddh, K. (2026) · Interdisciplinary biotechnological advances · doi:10.1007/978-981-95-8431-4_16
Integrative framework for selecting gene delivery technologies in microalgae for synthetic biology
Cho, M., & Sung, Y. J. (2026) · Molecular Biology Reports · doi:10.1007/s11033-026-12553-9
- Authors
- Jaeger, D., Hübner, W., Huser, T., Mussgnug, J. H., & Kruse, O.
- Affiliations
- Bielefeld University (DE)
- Corresponding author
- Kruse, Olaf
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2017-05
- Volume
- 249
- Pages
- 10-15
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W2596988300
- Semantic Scholar
- a1761bdea2d34813effc60e0354b1362a88115ed
- Cited by
- 31
- References
- 58
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 2
- FWCI
- 0.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- PUB – Publications at Bielefeld University (Bielefeld University) (submittedVersion)
- Publikationen an der Universität Bielefeld (Universität Bielefeld) (submittedVersion)
- Funders
- German state North Rhine-Westphalia
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- oleaginous microalgae, nuclear transformation, electroporation, reporter transgenes, photosynthetic microalgae, non-edible feedstock
- MeSH terms
- Chlorophyta, Biotechnology, Luminescent Proteins, Plasmids, Recombinant Proteins, Transformation, Genetic, Electroporation, Microalgae
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