KNOX1 is expressed and epigenetically regulated during in vitro conditions in Agave spp
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BACKGROUND: The micropropagation is a powerful tool to scale up plants of economical and agronomical importance, enhancing crop productivity. However, a small but growing body of evidence suggests that epigenetic mechanisms, such as DNA methylation and histone modifications, can be affected under the in vitro conditions characteristic of micropropagation. Here, we tested whether the adaptation to different in vitro systems (Magenta boxes and Bioreactors) modified epigenetically different clones of Agave fourcroydes and A. angustifolia. Furthermore, we assessed whether these epigenetic changes affect the regulatory expression of KNOTTED1-like HOMEOBOX (KNOX) transcription factors. RESULTS: To gain a better understanding of epigenetic changes during in vitro and ex vitro conditions in Agave fourcroydes and A. angustifolia, we analyzed global DNA methylation, as well as different histone modification marks, in two different systems: semisolid in Magenta boxes (M) and temporary immersion in modular Bioreactors (B). No significant difference was found in DNA methylation in A. fourcroydes grown in either M or B. However, when A. fourcroydes was compared with A. angustifolia, there was a two-fold difference in DNA methylation between the species, independent of the in vitro system used. Furthermore, we detected an absence or a low amount of the repressive mark H3K9me2 in ex vitro conditions in plants that were cultured earlier either in M or B. Moreover, the expression of AtqKNOX1 and AtqKNOX2, on A. fourcroydes and A. angustifolia clones, is affected during in vitro conditions. Therefore, we used Chromatin ImmunoPrecipitation (ChIP) to know whether these genes were epigenetically regulated. In the case of AtqKNOX1, the H3K4me3 and H3K9me2 were affected during in vitro conditions in comparison with AtqKNOX2. CONCLUSIONS: Agave clones plants with higher DNA methylation during in vitro conditions were better adapted to ex vitro conditions. In addition, A. fourcroydes and A. angustifolia clones displayed differential expression of the KNOX1 gene during in vitro conditions, which is epigenetically regulated by the H3K4me3 and H3K9me2 marks. The finding of an epigenetic regulation in key developmental genes will make it important in future studies to identify factors that help to find climate-resistant micropropagated plants.
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In Agave fourcroydes and A. angustifolia, no significant differences in global DNA methylation were found between clones grown in Magenta boxes and bioreactors, but a two-fold difference was found between the two species independent of the in vitro system used.
“A global DNA methylation level analysis revealed no significant differences between the A. fourcroydes clones grown in either M or B in vitro systems, but, when the two agave species were compared, there was a two-fold difference between them, independent of the in vitro system used.”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as de-la-Pena et al. (2012) [126]
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“(2012) [126] analyzed whether the adaptation to different in vitro culture systems (Magenta boxes—M and Bioreactors—B) epigenetically modifies different clones of Agave fourcroydes and A.”
BackgroundAgavebiotechnology: an overview
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Self-citationTowards the Understanding of Important Coconut Endosperm Phenotypes: Is there an Epigenetic Control?
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An efficient immunodetection method for histone modifications in plants
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Self-citationEpigenetic changes and photosynthetic plasticity in response to environment
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Somatic Embryogenesis: Polycomb Complexes Control Cell-to-Embryo Transition
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- Authors
- De-la-Peña, C., Nic-Can, G., Ojeda, G., Herrera-Herrera, J. L., López-Torres, A., Wrobel, K., & Robert-Díaz, M. L.
- Affiliations
- Autonomous University of Yucatán (MX)
- Centro de Investigación Científica de Yucatán, A.C (MX)
- Universidad de Guanajuato (MX)
- Venue
- BMC Plant Biology
- Published
- 2012
- Volume
- 12
- Issue
- 1
- Pages
- 203
- ISSN
- 1471-2229 (ISSN-L)
- PMC
- PMC3541254
- OpenAlex
- W1967932067
- Cited by
- 43
- References
- 89
- Citations by year
- 2026: 3 · 2025: 2 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 6 · 2019: 6 · 2018: 4 · 2017: 1 · 2016: 2 · 2015: 7 · 2014: 4 · 2013: 2
- FWCI
- 1.67
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- BMC Plant Biology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Consejo Nacional de Ciencia y Tecnología (CB2012)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- Agave fourcroydes, Agave angustifolia, KNOX transcription factors, DNA methylation, histone modifications, H3K4me3, H3K9me2, epigenetic regulation, in vitro adaptation, ex vitro acclimatization, bioreactors
- MeSH terms
- Histones, Lysine, Plant Proteins, Immunoblotting, Homeodomain Proteins, Gene Expression Regulation, Plant, DNA, Plant, Genome, Plant, DNA Methylation, Agave, Epigenesis, Genetic, Chromatin Immunoprecipitation, Real-Time Polymerase Chain Reaction
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