Scenedesmus obliquus metabolomics: effect of photoperiods and cell growth phases

Vendruscolo, Raquel Guidetti; Fagundes, Mariane Bittencourt; Maroneze, Mariana Manzoni; do Nascimento, Tatiele Casagrande; de Menezes, Cristiano Ragagnin; Barin, Juliano Smanioto; Zepka, Leila Queiroz; Jacob-Lopes, Eduardo; Wagner, Roger

doi:10.1007/s00449-019-02076-y

Cited by 45Algal biology and biofuel productionAquatic Ecosystems and Phytoplankton DynamicsAquaculture Nutrition and Growth

Scenedesmus obliquus metabolomics: effect of photoperiods and cell growth phases

Vendruscolo, Raquel Guidetti, Fagundes, Mariane Bittencourt, Maroneze, Mariana Manzoni, do Nascimento, Tatiele Casagrande, de Menezes, Cristiano Ragagnin, Barin, Juliano Smanioto, Zepka, Leila Queiroz, Jacob-Lopes, Eduardo +1 more·2019·Bioprocess and Biosystems Engineering

DOI 10.1007/s00449-019-02076-y

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Abstract

Environmental factors directly affect the growth and composition of microalgal biomass. Therefore, the present work analyzed the metabolomics (amino acids, organic acids, and fatty acids) of the microalga Scenedesmus obliquus cultivated in 24:0 and 12:12 (light:dark) photoperiods and different phases of cell growth. Furthermore, the metabolites were related to protein, lipid, and chlorophyll contents at the end of cultivation. The highest biomass concentration (4020 mg L- 1) and protein (47.3%) were obtained in culture under constant illumination. The cultivation 12:12 (light:dark) photoperiod triggered higher production of lipids (23.0%) and chlorophylls (26.4 mg g- 1) by S. obliquus. Microalgal metabolites were greatly affected by photoperiod and by phase of cell growth. Thus, metabolite production could be related to both the environmental conditions under which cultivation occurred and to the different concentrations of products (proteins, lipids, and chlorophylls) present in the S. obliquus biomass.

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Scenedesmus obliquus grown under a 12:12 light/dark photoperiod had 20% more total chlorophylls (26.4 mg/g) compared to continuous lighting.
“Vendruscolo et al. (2019) demonstrated that Scenedesmus obliquus grown under a 12:12 light/dark photoperiod had 34% higher lipid content (23.0%) and 20% more total chlorophylls (26.4 mg/g) compared to continuous lighting, although continuous light favored protein accumulation (47.3%).”
From Microalgae biotechnology and its role in sustainable and healthy food design (2025) · cites this paper as Vendruscolo et al., 2019
Continuous light favored protein accumulation (47.3%) in Scenedesmus obliquus compared to a 12:12 light/dark photoperiod.
“Vendruscolo et al. (2019) demonstrated that Scenedesmus obliquus grown under a 12:12 light/dark photoperiod had 34% higher lipid content (23.0%) and 20% more total chlorophylls (26.4 mg/g) compared to continuous lighting, although continuous light favored protein accumulation (47.3%).”
From Microalgae biotechnology and its role in sustainable and healthy food design (2025) · cites this paper as Vendruscolo et al., 2019
Scenedesmus obliquus grown under a 12:12 light/dark photoperiod had 34% higher lipid content (23.0%) compared to continuous lighting.
“Vendruscolo et al. (2019) demonstrated that Scenedesmus obliquus grown under a 12:12 light/dark photoperiod had 34% higher lipid content (23.0%) and 20% more total chlorophylls (26.4 mg/g) compared to continuous lighting, although continuous light favored protein accumulation (47.3%).”
From Microalgae biotechnology and its role in sustainable and healthy food design (2025) · cites this paper as Vendruscolo et al., 2019

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Authors: Vendruscolo, R. G., Fagundes, M. B., Maroneze, M. M., do Nascimento, T. C., de Menezes, C. R., Barin, J. S., Zepka, L. Q., Jacob-Lopes, E., & Wagner, R.
Affiliations: Universidade Federal de Santa Maria (BR)
Corresponding author: Wagner, Roger
ORCID: Vendruscolo, Raquel Guidetti
Fagundes, Mariane Bittencourt
Maroneze, Mariana Manzoni
Barin, Juliano Smanioto
Zepka, Leila Queiroz
Wagner, Roger
Venue: Bioprocess and Biosystems Engineering
Publisher: Springer Science and Business Media LLC
Published: 2019-01-22
Volume: 42
Issue: 5
Pages: 727-739
ISSN: 1615-7591 (ISSN-L)
1615-7605
DOI: 10.1007/s00449-019-02076-y
OpenAlex: W2912253092
Cited by: 45
References: 46
Citations by year: 2026: 7 · 2025: 8 · 2024: 8 · 2023: 6 · 2022: 2 · 2021: 4 · 2020: 6 · 2019: 4
FWCI: 0.85
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Bioprocess and Biosystems Engineering (publishedVersion)
PubMed (publishedVersion)
Funders: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
Topics: Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
Keywords: Scenedesmus obliquus, photoperiod, cell growth phase, amino acids, organic acids, fatty acids, microalgal biomass, lipid production, chlorophyll content, protein content, microalgae cultivation
MeSH terms: Chlorophyll, Fatty Acids, Photoperiod, Biomass, Scenedesmus, Metabolomics, Microalgae

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Scenedesmus obliquus metabolomics: effect of photoperiods and cell growth phases

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