Effects of anandamide in migraine: data from an animal model
Open accessAbstract
Systemic nitroglycerin (NTG) produces spontaneous-like migraine attacks in migraine sufferers and induces a condition of hyperalgesia in the rat 4 h after its administration. Endocannabinoid system seems to be involved in the modulation of NTG-induced hyperalgesia, and probably, in the pathophysiological mechanisms of migraine. In this study, the analgesic effect of anandamide (AEA) was evaluated by means of the formalin test, performed in baseline conditions and following NTG-induced hyperalgesia in male Sprague-Dawley rats. AEA was administered 30 min before the formalin injection. In addition, the effect of AEA (administered 30 min before NTG injection) was investigated on NTG-induced Fos expression and evaluated 4 h following NTG injection. AEA induced a significant decrease in the nociceptive behavior during both phases of the formalin test in the animals treated with vehicle, while it abolished NTG-induced hyperalgesia during the phase II. Pre-treatment with AEA significantly reduced the NTG-induced neuronal activation in nucleus trigeminalis caudalis, confirming the results obtained in our previous study, and in area postrema, while the same treatment induced an increase of Fos expression in paraventricular and supraoptic nuclei of the hypothalamus, parabrachial nucleus, and periaqueductal grey. The study confirms that a dysfunction of the endocannabinoid system may contribute to the development of migraine attacks and that a pharmacological modulation of CB receptors can be useful for the treatment of migraine pain.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A dysfunction of the endocannabinoid system may contribute to the development of migraine attacks, and modulation of this system can be useful for the treatment of pain associated with migraine.
“These studies suggest that a dysfunction of the endocannabinoid system may contribute to the development of migraine attacks and modulation of this system can be useful for the treatment of pain associated to migraine.”
From The endogenous cannabinoid system: a budding source of targets for treating inflammatory and neuropathic pain (2018) · cites this paper as Greco et al, 2011
AEA abolishes nitroglycerin-induced hyperalgesia in the phase II of the formalin test.
“AEA also abolishes nitroglycerin-induced hyperalgesia in the phase II of formalin test (Greco et al, 2011).”
From The endogenous cannabinoid system: a budding source of targets for treating inflammatory and neuropathic pain (2018) · cites this paper as Greco et al, 2011
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
The formalin test: an evaluation of the method
Tjølsen, A., Berge, O., Hunskaar, S., Rosland, J. H., & Hole, K. (1992) · Pain · doi:10.1016/0304-3959(92)90003-t · Cited by 2,434 · see citing works
Induction of c-fos-like protein in spinal cord neurons following sensory stimulation
Hunt, S. P., Pini, A., & Evan, G. I. (1987) · Nature · doi:10.1038/328632a0 · Cited by 1,986 · see citing works
Isaacson, P. G., Doǧan, A., Price, S. K., & Spencer, J. (1989) · The American Journal of Surgical Pathology · doi:10.1097/00000478-198912000-00004 · Cited by 1,913 · see citing works
Immunohistochemical distribution of cannabinoid CB1 receptors in the rat central nervous system
Tsou, K., Brown, S., Sañudo-Peña, M. C., Mackie, K., & Walker, J. M. (1998) · Neuroscience · doi:10.1016/s0306-4522(97)00436-3 · Cited by 1,586 · see citing works
Identification and Functional Characterization of Brainstem Cannabinoid CB<sub>2</sub>Receptors
Van Sickle, M. D., Duncan, M., Kingsley, P. J., Mouihate, A., Urbani, P., Mackie, K., Stella, N., Makriyannis, A., Piomelli, D., Davison, J. S., et al. (2005) · Science · doi:10.1126/science.1115740 · Cited by 1,470 · see citing works
Cannabinoid receptors and pain
Pertwee, R. G. (2001) · Progress in Neurobiology · doi:10.1016/s0301-0082(00)00031-9 · Cited by 741 · see citing works
Mailleux, P., & Vanderhaeghen, J. (1992) · Neuroscience · doi:10.1016/0306-4522(92)90409-u · Cited by 663 · see citing works
Uddman, R., Edvinsson, L. I., Ekman, R., Kingman, T. A., & McCulloch, J. (1985) · Neuroscience Letters · doi:10.1016/0304-3940(85)90296-4 · Cited by 409 · see citing works
The pharmacological activity of anandamide, a putative endogenous cannabinoid, in mice.
Smith, P. B., Compton, D. M., Welch, S. P., Razdan, R. K., Mechoulam, R., & Martin, B. R. (1994) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)22356-4 · Cited by 394 · see citing works
CB2 cannabinoid receptor-mediated peripheral antinociception
Malan, P. T., Ibrahim, M. M., Deng, H., Liu, Q., Mata, H. P., Vanderah, T., Porreca, F., & Makriyannis, A. (2001) · Pain · doi:10.1016/s0304-3959(01)00321-9 · Cited by 391 · see citing works
Jaggar, S., Hasnie, F. S., Sellaturay, S., & Rice, A. S. C. (1998) · Pain · doi:10.1016/s0304-3959(98)00041-4 · Cited by 351 · see citing works
Localization of the CB1 cannabinoid receptor in the rat brain. An immunohistochemical study☆
Moldrich, G., & Wenger, T. (2000) · Peptides · doi:10.1016/s0196-9781(00)00324-7 · Cited by 335 · see citing works
Endocannabinoids and the regulation of their levels in health and disease
Marzo, V. D., & Petrosino, S. (2007) · Current Opinion in Lipidology · doi:10.1097/mol.0b013e32803dbdec · Cited by 289 · see citing works
Antihyperalgesic effects of spinal cannabinoids
Richardson, J. D., Aanonsen, L., & Hargreaves, K. (1998) · European Journal of Pharmacology · doi:10.1016/s0014-2999(97)01621-x · Cited by 275 · see citing works
Spinal and supraspinal components of cannabinoid-induced antinociception.
Lichtman, A. H., & Martin, B. R. (1991) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)20242-7 · Cited by 218 · see citing works
An examination of the central sites of action of cannabinoid-induced antinociception in the rat
Martin, W. J., Patrick, S. L., Coffin, P. O., Tsou, K., & Walker, J. M. (1995) · Life Sciences · doi:10.1016/0024-3205(95)00195-c · Cited by 165 · see citing works
Akerman, S., Kaube, H., & Goadsby, P. J. (2004) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.103.059808 · Cited by 158 · see citing works
Sepers, M. D., Oland, L. D., Mackie, K., Davison, J. S., & Sharkey, K. A. (2003) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00113.2003 · Cited by 141 · see citing works
Biochemistry of the Endogenous Ligands of Cannabinoid Receptors
Marzo, V. D., & Deutsch, D. G. (1998) · Neurobiology of Disease · doi:10.1006/nbdi.1998.0214 · Cited by 127 · see citing works
Levels of nerve growth factor in cerebrospinal fluid of chronic daily headache patients
Sarchielli, P., Alberti, A., Floridi, A., & Gallai, V. (2001) · Neurology · doi:10.1212/wnl.57.1.132 · Cited by 126 · see citing works
Nitroglycerin induces hyperalgesia in rats—a time-course study
Tassorelli, C., Greco, R., Wang, D., Sandrini, M., Sandrini, G., & Nappi, G. (2003) · European Journal of Pharmacology · doi:10.1016/s0014-2999(03)01421-3 · Cited by 107 · see citing works
Self-citationNackley, A. G., Suplita, R. L., & Hohmann, A. G. (2003) · Neuroscience · doi:10.1016/s0306-4522(02)00870-9 · Cited by 103 · see citing works
Tassorelli, C., Greco, R., Morazzoni, P., Riva, A., Sandrini, G., & Nappi, G. (2005) · Cephalalgia · doi:10.1111/j.1468-2982.2005.00915.x · Cited by 91 · see citing works
Self-citationRole of calcitonin gene-related peptide and substance P in different models of pain.
Greco, R., Tassorelli, C., Sandrini, G., Bella, P. D., Buscone, S., & Nappi, G. (2008) · PubMed · doi:10.1111/j.1468-2982.2007.01468.x · Cited by 85 · see citing works
Self-citationThe effects on the central nervous system of nitroglycerin—putative mechanisms and mediators
Tassorelli, C., Joseph, S., Buzzi, M. G., & Nappi, G. (1999) · Progress in Neurobiology · doi:10.1016/s0301-0082(98)00071-9 · Cited by 84 · see citing works
Self-citationPárdutz, Á., Multon, S., Malgrange, B., Párducz, Á., Vécsei, L., & Schoenen, J. E. (2002) · European Journal of Neuroscience · doi:10.1046/j.1460-9568.2002.02031.x · Cited by 83 · see citing works
Welch, S. P., Dunlow, L. D., Patrick, G., & Razdan, R. K. (1995) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)09617-x · Cited by 79 · see citing works
Greco, R., Gasperi, V, Sandrini, G., Bagetta, G., Nappi, G., Maccarrone, M., & Tassorelli, C. (2010) · Cephalalgia · doi:10.1111/j.1468-2982.2009.01924.x · Cited by 64 · see citing works
Self-citationA comparison of Δ9-THC and anandamide induced c-fos expression in the rat forebrain
McGregor, I. S., Arnold, J. C., Weber, M. F., Topple, A. N., & Hunt, G. E. (1998) · Brain Research · doi:10.1016/s0006-8993(98)00549-6 · Cited by 59 · see citing works
Tassorelli, C., Joseph, S. A., & Nappi, G. (1997) · Neuropharmacology · doi:10.1016/s0028-3908(97)00122-6 · Cited by 55 · see citing works
Self-citationTassorelli, C., Greco, R., Wang, D., Sandrini, G., & Nappi, G. (2006) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2006.01.023 · Cited by 46 · see citing works
Self-citationCosta, B., Vailati, S., & Colleoni, M. (1999) · Behavioural Pharmacology · doi:10.1097/00008877-199905000-00009 · Cited by 44 · see citing works
Patel, N. A., Moldow, R. L., Patel, J. A., Wu, G.-D., & Chang, S. L. (1998) · Brain Research · doi:10.1016/s0006-8993(98)00364-3 · Cited by 42 · see citing works
Buzzi, M. G., Tassorelli, C., & Nappi, G. (2003) · Cephalalgia · doi:10.1046/j.1468-2982.23.s1.1.x · Cited by 36 · see citing works
Self-citationThe (endo)cannabinoid system in multiple sclerosis and amyotrophic lateral sclerosis.
Centonze, D., Rossi, S., Finazzi-Agrò, A., Bernardi, G., & Maccarrone, M. (2007) · International review of neurobiology · doi:10.1016/s0074-7742(07)82009-5 · Cited by 29 · see citing works
Self-citationSarchielli, P., Alberti, A., Gallai, B., et al. (2002) · The Journal of Headache and Pain · doi:10.1007/s101940200030 · Cited by 18 · see citing works
Potential Neurogenic and Vascular Roles of Nitric Oxide in Migraine Headache and Aura
Myers, D. E. (1999) · Headache The Journal of Head and Face Pain · doi:10.1046/j.1526-4610.1999.3902118.x · Cited by 12 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Donvito, G., Nass, S. R., Wilkerson, J. L., Curry, Z. A., Schurman, L. D., Kinsey, S. G., & Lichtman, A. H. (2018) · Neuropsychopharmacology · doi:10.1038/npp.2017.204 · Cited by 301 · see citing works
Greco, R., Bandiera, T., Mangione, A., Demartini, C., Siani, F., Nappi, G., Sandrini, G., Guijarro, A., Armirotti, A., Piomelli, D., et al. (2015) · Cephalalgia · doi:10.1177/0333102414566862 · Cited by 63 · see citing works
Self-citationGreco, R., Mangione, A. S., Sandrini, G., Nappi, G., & Tassorelli, C. (2014) · The Journal of Headache and Pain · doi:10.1186/1129-2377-15-14 · Cited by 46 · see citing works
Self-citation
- Authors
- Greco, R., Mangione, A. S., Sandrini, G., Maccarrone, M., Nappi, G., & Tassorelli, C.
- Affiliations
- University of Pavia (IT)
- University of Teramo (IT)
- Corresponding author
- Greco, Rosaria
- Venue
- The Journal of Headache and Pain
- Published
- 2011-02-18
- Volume
- 12
- Issue
- 2
- Pages
- 177-183
- ISSN
- 1129-2369 (ISSN-L)
- 1129-2377
- PMC
- PMC3072518
- OpenAlex
- W2093129801
- Cited by
- 48
- References
- 38
- Citations by year
- 2026: 3 · 2025: 3 · 2023: 3 · 2022: 4 · 2021: 5 · 2020: 5 · 2019: 7 · 2018: 2 · 2017: 6 · 2016: 1 · 2015: 3 · 2014: 3 · 2013: 1 · 2012: 1
- FWCI
- 1.31
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- The Journal of Headache and Pain (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Archivio Istituzionale della Ricerca - Università degli Studi dell Aquila (submittedVersion)
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Migraine and Headache Studies
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Keywords
- anandamide, migraine, endocannabinoid system, analgesia, formalin test, Fos expression, nucleus tractus solitarius, area postrema, hypothalamus, parabrachial nucleus, periaqueductal gray
- MeSH terms
- Analgesics, Animals, Arachidonic Acids, Behavior, Animal, Disease Models, Animal, Drug Interactions, Nitroglycerin, Male, Migraine Disorders, Pain Measurement, Time Factors, Vasodilator Agents, Treatment Outcome, Rats, Sprague-Dawley, Rats, Polyunsaturated Alkamides, Cannabinoid Receptor Modulators, Endocannabinoids
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
