Cited by 19Fungal and yeast genetics researchMicrobial Natural Products and BiosynthesisCancer, Lipids, and Metabolism

Cloning of the Rhizomucor miehei 3-hydroxy-3-methylglutaryl-coenzyme A reductase gene and its heterologous expression in Mucor circinelloides

Lukács, Gyöngyi, Papp, Tamás, Somogyvári, Ferenc, Csernetics, Árpád, Nyilasi, Ildikó, Vágvölgyi, Csaba·2009·Antonie van Leeuwenhoek

Abstract

In this study, the gene hmgR encoding the 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) was cloned and characterized in the zygomycete fungus Rhizomucor miehei. The hmgR gene comprises a total of 3,585 bp including the coding sequence of a 1,058 amino acids length putative protein and five introns (137, 83, 59, 60 and 69 bp in length) dispersed in the whole coding region. Southern hybridization analysis revealed that the gene is present only in one copy in the R. miehei genome. The isolated Rhizomucor gene was expressed in the related fungus, Mucor circinelloides. Transformants harbouring the Rhizomucor hmgR gene in an autoreplicative plasmid proved to be more tolerant to statins (e.g. lovastatin, simvastatin, and fluvastatin), the competitive inhibitors of the HMG-CoA reductase, than the original M. circinelloides strain. At the same time, heterologous expression of the Rhizomucor hmgR did not affect the carotenoid production of M. circinelloides.

Integrity signals

Registry

Crossref · 10.1007/s10482-008-9287-2

Cites

41 of 41 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Lukács, G., Papp, T., Somogyvári, F., Csernetics, Á., Nyilasi, I., & Vágvölgyi, C.
Affiliations
University of Szeged (HU)
Corresponding author
Papp, Tamás
Venue
Antonie van Leeuwenhoek
Published
2008-10-14
Volume
95
Issue
1
Pages
55-64
ISSN
  • 0003-6072 (ISSN-L)
  • 1572-9699
OpenAlex
W1982386942
Cited by
19
References
41
Citations by year
2023: 3 · 2022: 1 · 2019: 3 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 2
FWCI
0.68
Type
journal article
Language
en
Open access
closed
Locations
  • Antonie van Leeuwenhoek (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
Keywords
Rhizomucor miehei, Mucor circinelloides, HMG-CoA reductase, gene cloning, heterologous expression, lovastatin, simvastatin, fluvastatin, carotenoid production, introns
MeSH terms
Amino Acid Sequence, Cloning, Molecular, Fungal Proteins, Fungi, Molecular Sequence Data, Mucor, Phylogeny, Gene Expression, Sequence Alignment, Protein Structure, Tertiary, Rhizomucor, Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review