Anti-inflammatory Effects of Oleanolic Acid on LPS-Induced Inflammation In Vitro and In Vivo
Abstract
Oleanolic acid (OA) is a triterpenoid known for its anti-inflammatory and anti-cancer properties; however, the anti-inflammatory effects of OA on lipopolysaccharide (LPS)-mediated pro-inflammatory responses have not been studied. Here, we first investigated the possible anti-inflammatory effects of OA against pro-inflammatory responses in human umbilical vein endothelial cells (HUVECs) induced by LPS and the associated signaling pathways. We found that OA inhibited LPS-induced barrier disruption, expression of cell adhesion molecules (CAMs), and adhesion/transendothelial migration of monocytes to HUVECs. OA also suppressed acetic acid-induced hyperpermeability and carboxymethylcellulose-induced leukocyte migration in vivo. Further studies revealed that OA suppressed the production of tumor necrosis factor-α and activation of nuclear factor-κB by LPS. Collectively, these results suggest that OA has anti-inflammatory effects by inhibiting hyperpermeability, the expression of CAMs, and the adhesion and migration of leukocytes, thereby endorsing its usefulness as a therapeutic agent for vascular inflammatory diseases.
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These trials were carried out in lipopolysaccharide challenged endothelial cells or in animal models of different pathologies such as hypertension or obesity, involving inflammatory processes.
“These trials were carried out in lipopolysaccharide challenged endothelial cells [39] or in animal models of different pathologies such as hypertension [7] or obesity [8], involving inflammatory processes.”
From Olive pomace oil can improve blood lipid profile: a randomized, blind, crossover, controlled clinical trial in healthy and at-risk volunteers (2022) · cites this paper as [39]
Triterpenic acids (OA, MA and UA) have been reported to suppress the expression of E-selectin, ICAM-1 and VCAM-1 in different cultured human endothelial cells.
“Triterpenic acids (OA, MA and UA) have been reported to suppress the expression of E-selectin, ICAM-1 and VCAM-1 in different cultured human endothelial cells [39–41], although none of these parameters showed variations after the intake of OPO and HOSO (Table 5).”
From Olive pomace oil can improve blood lipid profile: a randomized, blind, crossover, controlled clinical trial in healthy and at-risk volunteers (2022) · cites this paper as [39]
Previous assays have shown that triterpenes characteristic of OPO (OA and MA) modulate endothelial cell inflammation and suppress the production of inflammatory mediators such as TNFα and nuclear transcription factor kappa B (NF-κB) in in vivo and in vitro assays.
“Previous assays have shown that triterpenes characteristic of OPO (OA and MA) modulate endothelial cell inflammation and suppress the production of inflammatory mediators such as TNFα and nuclear transcription factor kappa B (NF-κB) in in vivo and in vitro assays [7, 8, 34, 39].”
From Olive pomace oil can improve blood lipid profile: a randomized, blind, crossover, controlled clinical trial in healthy and at-risk volunteers (2022) · cites this paper as [39]
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- Authors
- Lee, W., Yang, E.-J., Ku, S.-K., Song, K.-S., & Bae, J.-S.
- Affiliations
- Kyungpook National University (KR)
- Daegu Haany University (KR)
- Corresponding author
- Bae, Jong-Sup
- Venue
- Inflammation
- Published
- 2012-08-09
- Volume
- 36
- Issue
- 1
- Pages
- 94-102
- ISSN
- 0360-3997 (ISSN-L)
- 1573-2576
- OpenAlex
- W2043896458
- Cited by
- 145
- References
- 41
- Citations by year
- 2026: 3 · 2025: 15 · 2024: 17 · 2023: 14 · 2022: 13 · 2021: 15 · 2020: 16 · 2019: 10 · 2018: 2 · 2017: 9 · 2016: 14 · 2015: 7 · 2014: 7 · 2013: 2
- FWCI
- 2.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Inflammation (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacological Effects of Natural Compounds
- Keywords
- oleanolic acid, triterpenoids, anti-inflammatory activity, lipopolysaccharide, endothelial barrier disruption, adhesion molecules, monocyte adhesion, leukocyte transendothelial migration, hyperpermeability, leukocyte migration, TNF-α, NF-κB activation, vascular inflammation, human umbilical vein endothelial cells
- MeSH terms
- Animals, Anti-Inflammatory Agents, Non-Steroidal, Carboxymethylcellulose Sodium, Cell Adhesion, Cell Line, Cell Movement, Cell Survival, Endothelium, Vascular, Enzyme Activation, Female, Humans, Inflammation, Lipopolysaccharides, Mice, Inbred ICR, Oleanolic Acid, Tumor Necrosis Factor-alpha, NF-kappa B, Intercellular Adhesion Molecule-1, Vascular Cell Adhesion Molecule-1, E-Selectin, Acetic Acid, Mice, Transendothelial and Transepithelial Migration, Human Umbilical Vein Endothelial Cells
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